Six to eight weeks old BALB/c mice were purchased from Shanghai SLAC laboratory animal Co. Ltd. (Shanghai, China). NLRP3−/− mice had been described before (28 (link), 29 (link)). Caspase-1−/− mice were obtained from Jackson Laboratory. Both NLRP3−/− and caspase-1−/− mice had been crossed with BALB/c mice for 10 generations for the current project. To establish the murine model of AIH, ConA was injected by tail vein at a dose of 20 mg/kg body weight. RhIL-1Ra was given intraperitoneally at 20 mg/kg every 6 for 24 h before ConA injection, and the treatment with rhIL-1Ra was ongoing until sacrifice. NAC was intraperitoneally administrated 2 h before ConA injection at a dosage of 0.6 mg/kg. Mice were sacrificed at indicated time points for sampling of serum and liver. Blood obtained from orbital sinus was collected and stand for 1 h. The serum was separated by centrifuging at 3,000 rpm for 10 min at 4°C and used for the detection of liver function and cytokine levels.
Caspase 1 mice
Manufactured by Jackson ImmunoResearch
Caspase-1−/− mice are genetically engineered mice that have a targeted deletion of the caspase-1 gene. Caspase-1 is an enzyme involved in the activation of inflammatory cytokines. These mice can be used to study the role of caspase-1 in various biological processes.
Automatically generated - may contain errors
5 protocols using caspase 1 mice
1
Murine Model of Autoimmune Hepatitis
2
Genetically Modified Mice for Necroptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rip3−/− mice were kindly provided by Prof. Xiaodong Wang's group, and Caspase-1−/− mice were purchased from Jackson Laboratory. C57BL/6 mice were purchased from B&K Universal Group Limited (Shanghai, China), and Rip3−/−Caspase-1−/− mice were obtained by crossing Rip3−/− and Caspase-1−/− mice. Mice were kept under specific pathogen-free conditions at the Animal Center, Shanghai Public Health Clinical Center. All animal-related procedures were performed according to the Institutional Animal Care and Use Committee (IACUS).
3
Genetically Modified Mice for Inflammasome Research
4
NLRP3 and Caspase-1 Knockout Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NLRP3−/− and caspase-1−/− mice on a C57BL/6 background were purchased from Jackson Laboratory (Bar Harbor, ME). WT C57BL/6 mice were purchased from Charles River Japan Inc. (Atsugi, Japan) and used as the control strain for these two knockout mouse strains. Specific pathogen-free (Supplemental Table 3 ) 5-week-old male animals were used. All the mice were housed in polycarbonate cages with paper chip bedding. The cages were located in an air-conditioned biohazard room with a 12-h light-dark cycle.
All experiments were carried out under the control of animal research committee in accordance with The Guidelines on Animal Experiments in Osaka City University Graduate School of Medicine, Japanese Government Animal Protection and Management Law (No. 105), and Japanese Government Notification on Feeding and Safekeeping of Animals (No. 6). All experimental procedures were approved by the Animal Care Committee of Osaka City University Graduate School of Medicine (Approval number 14031). We repeated the entire set of mice experiments at least twice on different days.
All experiments were carried out under the control of animal research committee in accordance with The Guidelines on Animal Experiments in Osaka City University Graduate School of Medicine, Japanese Government Animal Protection and Management Law (No. 105), and Japanese Government Notification on Feeding and Safekeeping of Animals (No. 6). All experimental procedures were approved by the Animal Care Committee of Osaka City University Graduate School of Medicine (Approval number 14031). We repeated the entire set of mice experiments at least twice on different days.
5
Mouse Models for Immune Research
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!