Enzyme-linked immunosorbent assay (ELISA) kits were used to estimate serum cystatin C (R&D Systems, USA), neutrophil gelatinase-associated lipocalin (NGAL), NADPH oxidase, nuclear factor erythroid 2-related factor 2 (Nrf2) (Cusabio, China), hemeoxygenase-1(HO-1) (Enzo Life Sciences, Egypt), heat shock protein 90 (HSP 90) (Bioassay™, USA), nuclear factor kappa-B (NF-κB) (Eiaab, China) and tumor necrosis factor-α (TNF-α) (Raybio, USA). Reduced glutathione (GSH) and blood urea nitrogen (BUN) were estimated using glutathione reduced and blood urea nitrogen colorimetric kits, respectively (Biodiagnostic, Egypt). Total antioxidant capacity (TAC) was assessed by antioxidant assay kit (Zenbio, USA).
Antioxidant assay kit
Manufactured by ZenBio
Sourced in United States
The Antioxidant assay kit is a laboratory tool used to measure the antioxidant capacity of a sample. It provides a quantitative assessment of the ability of a substance to neutralize free radicals or reactive oxygen species.
Automatically generated - may contain errors
2 protocols using antioxidant assay kit
1
Serum Biomarkers of Oxidative Stress
2
ABTS Antioxidant Assay for GCF TAOC
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An ABTS (2,2'Azinobis3ethylbenzothiazoline 6sulfonic acid) antioxidant assay kit (ZenBio Inc., Durham, USA) was used to determine the GCF TAOC levels at the selected sites. The collected GCF samples were added to the wells of microtitercoated plates with a pipette. The plates were sealed and incubated for 2 h at room temperature (18-25°C). The reagents were added as per the manufacturer's instructions. Color development was monitored every 5 min. Posi tive wells turned blue in color. The determination of optical density in each well was performed using a microplate reader (Molecular Devices, San Jose, USA) at an absorbance of 405 nm.
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