The following C. difficile strains were obtained from the National Collection of Type Cultures: NCTC 13307, NCTC 11205, NCTC 11209 and NCTC 11204. Non-toxigenic C. difficile strain ATCC 43593 was obtained from the American Type Culture Collection. Other organisms used were from an in-house Lumora culture collection. C. difficile strains were cultured anaerobically on blood agar (Oxoid), Brazier’s agar (Oxoid) or in brain heart infusion (Difco). Other organisms were cultured in tryptone soya agar (Oxoid) or buffered peptone water broth (Oxoid).
Buffered peptone water broth
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Buffered peptone water broth is a general-purpose culture medium used for the enrichment and recovery of a wide variety of microorganisms. It provides a buffered, nutritious environment that supports the growth and survival of various bacterial species.
Automatically generated - may contain errors
Lab products found in correlation
Blood agar, by Thermo Fisher Scientific (1 mentions)
Tryptone soya agar, by Thermo Fisher Scientific (1 mentions)
Brazier s agar, by Thermo Fisher Scientific (1 mentions)
Nitrocefin, by Merck Group (1 mentions)
β galactosidase, by Merck Group (1 mentions)
Rapid alkaline phosphatase, by Merck Group (1 mentions)
Mueller hinton broth (mhb), by Thermo Fisher Scientific (1 mentions)
β lactamase, by Merck Group (1 mentions)
2 protocols using buffered peptone water broth
1
Diverse C. difficile Strains Cultivation
2
Antimicrobial Activity Evaluation
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Bacterial strains of E. coli (NCTC 12241, 11560, 13351) and S. aureus (NCTC 12981, 12973) were obtained from Pro-Lab Diagnostics (UK). Nitrocefin was obtained from Merck Millipore (UK). Chlorophenol red-β-D-galactopyranoside (CPRG), 5-bromo-6-chloro-3-indolyl phosphate p-toluidine salt (BCIP, magenta phosphate) or magenta phosphate, β-galactosidase, β-lactamase and rAPid alkaline phosphatase were all acquired from Sigma-Aldrich (UK). HMRZ-86 was purchased from Bio-Rad Laboratories (UK). Filter paper (Whatman grade 4) was bought from GE Healthcare Life Sciences (UK). Mueller-Hinton broth and Buffered Peptone Water broth were purchased from Oxoid Ltd, UK. Pasteurised cow's milk was purchased from local stores (Hull, UK).
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