Diabetes was induced in overnight fasted rats by i.p. injection of freshly prepared STZ (60 mg/kg, dissolved in 0.1 M cold citrate buffer; pH 4.5). The STZ-treated animals were allowed to drink 20% glucose solution for 24 h to overcome initial drug-induced hypoglycemic mortality. Three days after STZ injection, blood samples were collected through the tail vein and blood glucose levels were measured using a glucometer (OneTouch Horizon, LifeScan, Johnson & Johnson, CA, USA). Animals with blood glucose above 250 mg/dL were used for the study.
Onetouch horizon
Manufactured by LifeScan
Sourced in United States
The OneTouch Horizon is a lab equipment product designed for blood glucose monitoring. It provides a reliable and accurate way to measure blood glucose levels. The device is intended for professional use in healthcare settings.
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Lab products found in correlation
4 protocols using onetouch horizon
1
Diabetes Induction in Rats Using STZ
2
Oral Glucose Tolerance Test in Mice
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Oral glucose tolerance test (5 g/kg BW) was performed on normoglycemic mice (n = 9), followed by measurement of blood glucose level (mg/dl) using OneTouch Horizon (Lifescan, Johnson and Johnson, USA). Polysorbate 80 (25 ml/kg BW, UNIVAR, Australia) and distilled water were used as negative controls; Solosa (1.67 μg/kg BW, Glimepiride solosa, Aventis, Italy) dissolved in distilled H2O was used as positive control; and crude aqueous leaf extract (200 mg/kg BW, 100 mg/kg BW, 50 mg/kg BW and 25 mg/kg BW) dissolved in distilled water, DCM leaf extract (200 mg/kg BW, 100 mg/kg BW, 50 mg/kg BW and 25 mg/kg BW) dissolved in Polysorbate 80 (25 mg/kg BW) and 1 (200 mg/kg BW, 100 mg/kg BW, 50 mg/kg BW, and 25 mg/kg BW) dissolved in polysorbate 80 (25 ml/kg BW) as test samples were orally administered to male albino mice. Blood glucose was measured within a 3 h period at 30 min intervals. Blood glucose reduction was computed and was used in the statistical analysis.
3
Weekly Body Weight and Fasting Glucose
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In all animal experiments, body weights were measured once a week. At the end of the experimental period, animals were fasted for 16 h, blood samples were collected from the tail vein, and the blood glucose levels were measured using One Touch Horizon (LifeScan, USA).
4
Streptozotocin-Induced Diabetic Rat Model
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Diabetes was induced in overnight fasted rats by a single i.p. injection of freshly prepared streptozotocin (STZ) (60 mg/kg, dissolved in 0.1 M cold citrate buffer, pH 4.5) (10) . STZ was purchased from Sigma-Aldrich (St. Louis, MO, USA). The STZ-treated animals were allowed to drink 5% glucose solution instead of drinking water for the first 24 h after STZ challenge to overcome initial drug-induced hypoglycemic mortality. Three days after STZ injection, blood samples were collected and blood glucose levels were measured using a glucometer (OneTouch Horizon, LifeScan, Johnson & Johnson, CA, USA). Animals with blood glucose level above 250 mg/dl were used. After the maintenance for 4 weeks, the blood and urine samples from these rats were again tested for hyperglycemia and proteinuria. The urine protein was estimated by Biuret's method using a commercial kit (Diamond Diagnostic, Egypt). Rats with hyperglycemia (≥250 mg/dl) and proteinuria (≥8.0 mg/dl) at the end of 4 weeks post STZ injection were selected for further study.
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