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1 843 protocols using spss statistics for windows version 25

1

Analyzing Malaria Trends and Weather Correlations

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All data were entered into Microsoft Excel. The analysis of the data was done using SPSS Statistics for Windows version 25.0 (SPSS Inc., Chicago, II, USA). The incidence of malaria for the different years was gotten as the total number of malaria cases for that year. The monthly, yearly, and seasonal averages of climatic variables (rainfall, temperature and humidity) were calculated with Microsoft Excel 2019. The charts and plots were produced using Qtiplot. The correlation between weather parameters; temperature, humidity and rainfall in Muyuka Health District (MHD) and Tiko Health District and the monthly, seasonally and yearly number of malaria cases were performed by Spearman’s rank correlation. Also, the simple seasonal model in SPSS Statistics for Windows version 25.0 (SPSS Inc., Chicago, II, USA), which is applied on series with no trend and a simple seasonal effect that is constant over time, was used to predict the number of malaria cases for a two-year period (2018 and 2019), which also showed the stationary R-squared and ljung box statistics.
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2

Statistical Analysis of Experimental Groups

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All statistical analyses were performed using IBM SPSS Statistics for Windows, Version 25.0 software (IBM Corp. Released 2017. IBM SPSS Statistics for Windows, Version 25.0. Armonk, NY: IBM Corp.). The level of significance was assessed at p < 0.05. The results of all groups were analyzed using a one-way analysis of variance followed by Tukey’s post hoc test.
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3

MALDI-FT-ICR-MSI and LESA-MSI Protocol

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For MALDI-FT-ICR-MSI analysis, SCiLS 2019 cPro (Bruker Daltonik, Bremen, GmbH & CO. KG) was used for imaging processing and visualization. For MALDI-FT-ICR, LESA-MSI, and LC/MS2, statistical analysis was performed using IBM SPSS Statistics for Windows (Version 25) (SPSS, IBM Analytics, New York, USA). Normality of the data distribution was assessed using the Kolmogorov–Smirnov test and through inspection of histograms and Q-Q plots. Normally distributed data followed parametric testing (analysis of variance (ANOVA)) and results were expressed as mean ± SEM whereas data that did not assume normal distribution was analyzed using non-parametric alternatives (Kruskal–Wallis test with post hoc Mann–Whitney U) and were expressed as median and interquartile range. Statistical significance was determined from a p value of < 0.05. Bland-Alman plots were generated using IBM SPSS Statistics for Windows (Version 25) (SPSS, IBM Analytics, New York, USA) to determine the agreement between MALDI-FT-ICR-MSI and LESA-MSI analysis methods.
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4

Hormone Levels in Comparative Analyses

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Data are presented as median (range). In calculations, hormone concentrations below LOD were assigned the value LOD/2. The Mann-Whitney U test was used for comparisons between groups. The Wilcoxon matched-pairs signed-rank test was used for 2 dependent samples. Correlation analyses were performed using Spearman (rho) nonparametric rank correlation and R2. A P value < 0.05 was considered significant. The software IBM SPSS Statistics for Windows, version 25.0 (IBM Corp, Armonk, NY, USA) was used for statistical analyses. Figures were drawn using SPSS or Origin version 9.0 (OriginLab Corporation, Northampton, MA, USA).
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5

Exploring CTS Awareness in Junior Doctors

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A ten-question survey was designed and delivered to FY2 doctors who graduated in 2017
and completed their two-year postgraduate foundation training in 2019. The details
of the survey are shown in Table 1. The
survey focused on: a) student exposure to CTS within their undergraduate curriculum,
b) student exposure to CTS outside their undergraduate curriculum, c) exposure to
CTS as a doctor within their postgraduate foundation training, d) foundation doctor
exposure to CTS outside of postgraduate foundation training, and e) their
self-reported interests in CTS.
The survey was delivered online to junior doctors in 2019. The study was conducted
using the SurveyMonkey® platform and was distributed through junior doctor
social media groups and university societies. Medical schools with no graduates in
2017 and 2018 were excluded from our study. IBM Corp. Released 2017, IBM SPSS
Statistics for Windows, Version 25.0, Armonk, NY: IBM Corp. and Microsoft Excel
365® were used for data collection and Student’s t-test statistical analysis.
A P-value < 0.05 is deemed statistically significant.
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6

Evaluating Imaging-Pathology Correlation in Cancer

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The ICC was used to evaluate the agreement between different measurement techniques by one method. Cancer lesions on MRI and histology were matched, and correlated for size using the Pearson correlation. The plots of MRI–histology size differences against tumour size on histology were used to assess size discrepancies and to calculate the optimal tumour size and treatment margins for FT. The impact of intralesional heterogeneity on mpMRI performance was assessed using linear regression of MRI size with pathology size and HG cancer burden, and their interaction as independent predictors. Categorical data were compared between groups using the chi‐squared test and continuous data were tested using the two‐tailed Student’s t‐test. Statistical significance was defined as P < 0.05. The statistical software used was IBM SPSS Statistics for Windows version 25.0 (IBM Corp., Armonk, NY, USA) and MedCalc version 18.2 (MedCalc Software, Ostend, Belgium).
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7

Statistical Analysis of Experimental Data

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Statistical significance between groups was determined using Mann–Whitney U‐test of Student’s t‐test. Statistical analysis on growth curves was assessed using two‐way repeated measures ANOVA. Z‐scores of MRI and Mercox parameters were determined by normalization of each experimental mouse to the average and standard deviation of control mice. Pearson’s correlation coefficients were calculated to determine P‐values in gene expression data. Bar graphs and dot plots with error bars represent mean and ±SD, respectively. Box plots represent minimum, first quartile, median, third quartile, and maximum. Statistical analyses were performed using graphpad prism 6 (San Diego, CA, USA) and ibm spss statistics for Windows, version 25.0 (IBM Corp, Armonk, NY, USA). Tests were performed two‐tailed, and a P‐value < 0.05 was considered statistically significant.
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8

Archery Performance and Psychophysiological Response

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Statistical analysis was performed with IBM®SPSS® statistics for Windows, version 25.0 (IBM Corp, Armonk, NY, USA). Data are reported as mean ± standard deviation. Differences on archery performance (total score) were detected with a paired-samples t-test. The normality of data was assessed by the Kolmogorov–Smirnov test. All data were normally distributed, and comparisons on subjective feelings between trials and over time (baseline vs. end of archery competition) were made with a two-way Repeated Measures ANOVA (time point × trial). Heart rate response at rest and after arrow throw was also analyzed with two-way Repeated Measures ANOVA (time point × trial). Bonferroni post-hoc analysis was performed where necessary. Effect sizes were calculated using partial eta squared (η2) interpreted as 0.01 for small, 0.06 for moderate and 0.14 for large. Statistical significance was set at p < 0.05. Statistical power analysis was performed using the G*Power 3.1 power analysis software. Post hoc power analysis revealed that the sample size of the present study was adequate to provide statistical power of both heart rate and other main parameters of the study such as fatigue, concentration and thirst) with >90% power and with a significance level, α = 0.05.
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9

Assessing Postural Effects on Waveform Severity

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By first assessing the parameters in the rested position and comparing this with
the subsequent positions, each subject was effectively their own control. IBM
SPSS Statistics for Windows, version 25.0 (IBM Corp., Armonk, NY, USA) software
package was used for data analysis.
The waveform severity score provided ordinal data and was analysed by applying
the related-samples Friedman’s two-way ANOVA test with Bonferroni
correction.
In order to determine if the presence of symptoms significantly differed between
each position, the non-parametric, binary Cochran’s Q-test was performed.
The Kolmogorov–Smirnov test showed that most of the PSV data met the normal
distribution assumptions (p > 0.05). A one-way,
repeated-measures, within-subject ANOVA test, with Bonferroni correction, could
then be performed. Mauchly’s test of sphericity was used to determine the
Greenhouse-Geisser epsilon estimate of 0.548 which allowed PSV pairwise
comparisons to be made from the ANOVA test.
The SRT dataset was checked for normality with the Kolmogorov Smirnov test. The
significance level for all but one position was p < 0.05 meaning the overall
SRT dataset was not normally distributed. The nonparametric, Friedman’s two-way
ANOVA was therefore performed.
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10

Chronic Total Occlusion PCI Outcomes

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Study participants were categorized according to the baseline eGFR. The baseline characteristics, angiographic characteristics and procedural details, intraprocedural and in-hospital complications were shown in Tables 13. Continuous variables are presented as the mean ± standard deviation or the median and interquartile range, whereas categorical variables are presented as percentages. Continuous variables were compared with the ANOVA test (normal distribution) or Kruskal–Wallis H test (abnormal distribution). Categorical variables were compared with the chi-square test or Kruskal–Wallis H test (NYHA functional class). The changes of symptoms and QOL (EQ-5D) were showed in Tables 5, 7. The comparison between two groups was used the student t-test while the comparison among four groups was used ANOVA test. Univariable and multivariable binary logistic regression analysis were performed to identify the risk factors of in-hospital complications and 1-year symptoms and QOL improvement in patients with low eGFR (<90 mL/min/1.73 m2) after successful CTO-PCI (Tables 4, 6, 8). A two-sided probability (p) value of <0.05 was considered significant. All calculations were performed with IBM-SPSS Statistics for Windows, version 25.0 (IBM Corporation, Armonk, NY, USA) and STATA 14 software (https://www.stata.com/stata14/).
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