Samples for TEM analysis were obtained by drop-casting a few microliters of solution onto standard TEM carbon-coated Cu-grids, and by allowing the solvent to fully evaporate. Samples were imaged by using a JEOL JEM 1011 TEM microscope (JEOL, Inc., Peabody, MA, USA) operating at 100 kV.
Jem 1011 tem microscope
Manufactured by JEOL
Sourced in United States
The JEM-1011 is a transmission electron microscope (TEM) manufactured by JEOL. It is a versatile and high-performance instrument designed for a wide range of applications in materials science, life sciences, and nanotechnology research. The JEM-1011 TEM provides high-resolution imaging capabilities, allowing for detailed observation and analysis of samples at the nanoscale level.
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Lab products found in correlation
7 protocols using jem 1011 tem microscope
1
TEM Sample Preparation and Imaging
2
Nanoparticle Morphology Characterization
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The external morphology of AgNPs, IM-PCL NPs, and HC-NPs was examined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Prior to SEM analysis, the samples were coated with a 10 nm gold layer. SEM analyses were taken with a Carl Zeiss Merlin SEM supplied with a Gemini II column and a field emission gun (FEG). In addition, AgNPs were analyzed using a JEOL Jem 1011 TEM microscope (Japan).
3
TEM Analysis of CaCO3 Particle Size and Morphology
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CaCO3 particle size and morphology were analyzed by the transmission electron microscopy (TEM) technique. CaCO3 samples were imaged by a JEOL JEM 1011 TEM microscope (JEOL USA, Inc., Peabody, MA, USA), operated at an acceleration voltage of 100 kV. CaCO3 samples were prepared by depositing a droplet of ethanol-dispersed particles onto a standard C-coated Cu grid. The average size of particle samples, as imaged by TEM, was determined by the ImageJ software (ImageJ, version 1.52t, Free Software for Image Data Analysis, imagej.nih.gov/ij/index.html , accessed on 22 March 2022). The number of analyzed particles exceeded 250 in each sample. Each point plotted on the graphs represents the average of 3 samples obtained under the same conditions.
4
Transmission Electron Microscopy of EVs
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Concentrated EVs were adsorbed to carbon-coated formvar grids for 15 min at RT. After a PBS wash, the grids were fixed in a 1% glutaraldehyde in PBS fixing buffer for 30 min at RT, followed by counterstaining with uranyl-oxalate. Grids were embedded in a mixture of 1.8% methylcellulose and 0.4% uranyl acetate at 4 °C and imaged on a Jeol JEM-1011 TEM microscope (Jeol).
5
Electron Microscopy of Extracellular Vesicles
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EVs were adsorbed to carbon-coated formvar grids for 15 min at room temperature. Unbound EVs were removed by a PBS wash. Grids were then fixed in a 2% paraformaldehyde, 0.2% glutaraldehyde in PBS fixing buffer for 30 min at room temperature, followed by counterstaining with uranyl-oxalate. Grids were then embedded in a mixture of 1.8% methyl cellulose and 0.4% uranyl acetate at 4 °C. Grids were imaged on a Jeol JEM-1011 TEM microscope (Jeol).
6
Transmission Electron Microscopy of EVs
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Concentrated EVs were adsorbed to carbon-coated formvar grids for 15 min at RT. After a PBS wash, the grids were fixed in a 1% glutaraldehyde in PBS fixing buffer for 30 min at RT, followed by counterstaining with uranyl-oxalate. Grids were embedded in a mixture of 1.8% methyl cellulose and 0.4% uranyl acetate at 4 °C and imaged on a Jeol JEM-1011 TEM microscope (Jeol).
7
Quantifying Nanoparticle Distribution in Heart Tissue
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Paraffin sections 6 μm thick were cut and stained with hematoxylin and eosin (H&E). Light microscopy and ultrastructural changes in the heart were assessed by experienced pathologists and electron microscopists (LCG, AGM, RRR), blind to the study group. TEM was performed in 31 age-matched control (n: 4) and (n: 27) human cases and 8 SW MMC and in control dogs.
Sections were stained with uranyl acetate and lead citrate and examined with a JEOL JEM-1011 TEM microscope, in order to identify the presence and locations of solid (non-volatile), primary NPs. EM evaluations were made from photomicrographs with final magnifications at 13x, 300x, 25,000x, 50,000x and 80,000x. An average of 1062±287 NPs was counted in targeted organelles and cells, comprising mitochondria, myofibers, cardiomyocyte nuclei, endothelium, and red blood cells (RBC), in both control and exposed cohorts. NP numbers were counted in 25 micrographs for each cohort, at a magnification of 83, 300 x, with an area of 34.37 µm 2 in each image.
Sections were stained with uranyl acetate and lead citrate and examined with a JEOL JEM-1011 TEM microscope, in order to identify the presence and locations of solid (non-volatile), primary NPs. EM evaluations were made from photomicrographs with final magnifications at 13x, 300x, 25,000x, 50,000x and 80,000x. An average of 1062±287 NPs was counted in targeted organelles and cells, comprising mitochondria, myofibers, cardiomyocyte nuclei, endothelium, and red blood cells (RBC), in both control and exposed cohorts. NP numbers were counted in 25 micrographs for each cohort, at a magnification of 83, 300 x, with an area of 34.37 µm 2 in each image.
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