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Qiacube ht plasticware

Manufactured by Qiagen
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The QIAcube HT Plasticware is a set of consumable laboratory equipment designed for use with the QIAcube HT automated sample processing system. The plasticware enables efficient and standardized sample handling and processing within the QIAcube HT platform.

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Lab products found in correlation

Qiaamp 96 dna qiacube ht kit, by Qiagen (7 mentions) Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (4 mentions) Cador pathogen 96 qiacube ht kit, by Qiagen (3 mentions) Nanodrop onec ultramicro ultraviolet spectrophotometer, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Shrimp alkaline phosphatase, by Thermo Fisher Scientific (1 mentions) Hi di formamide, by Thermo Fisher Scientific (1 mentions) Genescan 120 liz size standard, by Thermo Fisher Scientific (1 mentions) Genemapper software version 4, by Thermo Fisher Scientific (1 mentions) Snapshot ready reaction mix, by Thermo Fisher Scientific (1 mentions) Abi 7900ht fast, by Thermo Fisher Scientific (1 mentions) Sds 2, by Thermo Fisher Scientific (1 mentions)

10 protocols using qiacube ht plasticware

1

Interdental Brush DNA Isolation

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Total DNA was isolated from the interdental brushes using the QIAcube HT Plasticware and Cador Pathogen 96 QIAcube HT Kit (Qiagen, Hilden, Germany), according to the manufacturer’s guidelines. The elution volume used in this study was 150 μL. DNA quality and quantities were measured using an ultraviolet spectrophotometer at 260 and 280 nm. The DNA sample was considered pure if the A260/A280 ratio was in the range of 1.8–2 and the A260/A280 ratio was in the range of 2–2.2.
Inquimbert C., Bourgeois D., Bravo M., Viennot S., Tramini P., Llodra J.C., Molinari N., Dussart C., Giraudeau N, & Carrouel F. (2019). The Oral Bacterial Microbiome of Interdental Surfaces in Adolescents According to Carious Risk. Microorganisms, 7(9), 319.
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2

Plasma DNA Extraction and Purification

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Approximately 5 mL venous blood sample was drawn from each participant into tubes containing EDTA and centrifuged immediately at 3000× g for 5 min to separate plasma and serum. DNA was extracted from the plasma by the QIAcube HT Plasticware and QIAamp 96 DNA QIAcube HT Kit (Qiagen, Dusseldorf, Germany) following the manufacturer’s protocol and then stored at −80 °C until use. The A260/A280 of the purified DNA, tested by Nanodrop OneC Ultramicro ultraviolet spectrophotometer (Thermo Scientific, Waltham, MA, USA), was between 1.8 and 2.0, indicating that there was no external contamination.
Zhang H., Xu M., Zhao Q., Sun K., Gong W., Zhang Q., Zhu B, & An Y. (2016). Association between Polymorphism of Exportin-5 and Susceptibility to Lead Poisoning in a Chinese Population. International Journal of Environmental Research and Public Health, 14(1), 36.
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3

Interdental Brush DNA Isolation Using QIAcube

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Total DNA was isolated from the interdental brushes using the QIAcube® HT Plasticware and Cador® Pathogen 96 QIAcube® HT Kit (Qiagen, Hilden, Germany) according to the manufacturer’s guidelines. The elution volume used in this study was 150 μL. DNA quality and quantities were measured using an ultraviolet spectrophotometer. The DNA sample was considered pure if the A260/A280 ratio was in the range of 1.8–2 and the A260/A230 ratio was in the range of 2–2.2.
Bourgeois D., David A., Inquimbert C., Tramini P., Molinari N, & Carrouel F. (2017). Quantification of carious pathogens in the interdental microbiota of young caries-free adults. PLoS ONE, 12(10), e0185804.
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4

DNA and RNA Extraction from Blood and Tissue Samples

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A volume of 5-mL peripheral venous blood was collected from each participant for deoxyribonucleic acid (DNA) extraction by QIAcube HT Plasticware and QIAamp 96 DNA QIAcube HT Kit (Qiagen, Dusseldorf, Germany), following the manufacturer's protocol. The total RNA were extracted from 64 pairs of patients’ tumor and adjacent normal tissues from 1140 TC patients by TRIzol reagent (Invitrogen, Carlsbad, CA). All DNAs and RNAs were measured by Nanodrop-2000 spectrophotometer (Thermo, Waltham, MA) for their quality and quantity. For storage, DNAs were stored at −20 °C, while RNAs were stored at −80 °C for further processing.
Wen J., Gao Q., Wang N., Zhang W., Cao K., Zhang Q., Chen S, & Shi L. (2017). Association of microRNA-related gene XPO5 rs11077 polymorphism with susceptibility to thyroid cancer. Medicine, 96(14), e6351.
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5

DNA Extraction and Genotyping Protocol

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From each participant, 5 mL peripheral blood sample was collected and then fixed in EDTA for DNA isolation and genotyping. Genomic DNA from blood samples was extracted by QIAcube HT Plasticware and QIAamp 96 DNA QIAcube HT Kit (Qiagen, Dusseldorf, Germany) following manufacturer's protocol, and then stored at −80 °C.
Genotyping of SNPs was performed by SNaPshot assays. 2 μL PCR product was purified with 0.3 μL shrimp alkaline phosphatase (Thermo Fisher Scientific, MA) following manufacturer's protocol. After purification, production of SNaPshot extension reaction was mixed with 2 μL SNaPshot ready reaction mix (Applied Biosystems, CA) for further amplification. Hi-Di form amide and GeneScan-120 LIZ size standard (Applied Biosystems) were mixed with purified mini-sequencing products. Raw genomic data were collected using 3730 Genetic Analyzer Data Collection Software version 3.0 and analyzed with GeneMapper Software Version 4.1 (Applied Biosystems).
Zhang B., Zeng X., Xu Y., Zhang Y., Huang N., Gu Y., Shen X, & Liu X. (2017). The association between annexin A5 (ANXA5) gene polymorphism and left ventricular hypertrophy (LVH) in Chinese endogenous hypertension patients. Medicine, 96(44), e8305.
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6

Interdental Brush DNA Extraction

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Total DNA was isolated from the interdental brushes using the QIAcube® HT Plasticware and Cador® Pathogen 96 QIAcube® HT Kit (Qiagen, Hilden, Germany), according to manufacturer’s guidelines. The elution volume used in this study was 150 μL. DNA quality and quantities were measured using an ultraviolet spectrophotometer at 260 and 280 nm.
Carrouel F., Viennot S., Santamaria J., Veber P, & Bourgeois D. (2016). Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young Adults. Frontiers in Microbiology, 7, 840.
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7

Automated High-Throughput DNA Extraction from Bacterial Pellets

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The QIAcubeHT was prepared for the run with appropriate reagents from the QIAamp 96 DNA QIAcube HT kit (Qiagen part number 51331) and QIAcube HT Plasticware (Qiagen part number 950067) according to the instructions in the Setup Wizard in the QIAcube HT Software. The S-block containing sample lysates was placed on the QIAcube HT deck, and DNA extraction was performed using the Qiagen protocol “Gram-Bacterialpellets QCHT.” The program was modified so that two vacuum steps were performed during the protocol after the lysate was added to the QIAamp 96 filter plate to ensure all liquid passed through. Additionally, the QIAcube HT was programed to wait for user confirmation after both the first and second vacuum steps, so that the performing technician could visually confirm that all liquid passed through the QIAamp 96 filter plate before allowing the instrument to proceed to the next step. During the final step of the “Gram- Bacterialpellets QCHT” protocol, DNA was eluted from the QIAamp 96 filter plate with 70 µL 10 mM Tris-HCl pH 8.0.
Dickinson M.C., Wirth S.E., Baker D., Kidney A.M., Mitchell K.K., Nazarian E.J., Shudt M., Thompson L.M., Gubbala Venkata S.L., Musser K.A, & Mingle L. (2024). Implementation of a high-throughput whole genome sequencing approach with the goal of maximizing efficiency and cost effectiveness to improve public health. Microbiology Spectrum, 12(4), e03885-23.
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8

DNA Extraction and Genotyping of lncRNA MALAT1

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5 ml peripheral venous blood was collected from each participant. QIAcube HT Plasticware and QIAamp 96 DNA QIAcube HT Kit (Qiagen, Dusseldorf, Germany) were used for DNA extraction, following the manufacturer's protocol. The quality of DNA samples was evaluated based on the corresponding analysis on Nanodrop-2000 spectrophotometer (Thermo, Waltham, MA, USA). Genotyping of lnc MALAT1 polymorphisms was performed in TaqMan SNP Genotyping Assay using ABI Fast 7900HT real-time PCR system (Applied Biosystems, Foster City, CA, USA). The primer were synthesized and applied by Biolight Tec. (Nanjing, Jiangsu, China), and all primer and probe sequences were listed in the Supplementary table s1. SDS 2.4 software (Applied Biosystems) was used for allelic discrimination. Six samples were placed in each plate as the quality control. Additional10% samples were randomly chosen to repeat the genotyping, and the results were 100% consistent.
Wen J., Chen L., Tian H., Li J., Zhang M., Cao Q., Zhang W., Chen S, & Shi L. (2019). Effect of MALAT1 Polymorphisms on Papillary Thyroid Cancer in a Chinese Population. Journal of Cancer, 10(23), 5714-5721.
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9

Comprehensive Pathogen Detection Protocol

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Total DNA was isolated by Institut Clinident SAS (Aix en Provence, France) using the QIAcube® HT Plasticware and QIAamp® 96 DNA QIAcube® HT Kit (Qiagen, Hilden, Germany) ‡ according to manufacturer's guidelines. The elution volume used in this study was 150 µl.
qRT-PCR analysis for Entamoeba histolytica, Epstein Barr virus, Cytomegalovirus and Herpes virus type 1 and 2 was carried out by Espace Lab, Geneva, Swizerland.
Canullo L., Masucci L., Quaranta G., Patini R., Caponio V.C.A., Pesce P., Ravidà A., Penarrocha-Oltra D, & Penarrocha-Diago M. (2021). Culturomic and quantitative real-time-polymerase chain reaction analyses for early contamination of abutments with different surfaces: A randomized clinical trial. Clinical implant dentistry and related research, 23(4).
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10

Genomic DNA Extraction from Peripheral Blood

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A total of 5 ml peripheral blood was collected in EDTA and taken for DNA isolation and genotyping. Genomic DNA was extracted from the blood samples of subjects by using the QIAcube HT Plasticware and QIAamp 96 DNA QIAcube HT Kit ® (Qiagen, Dusseldorf, Germany) following the manufacturer's protocol and then stored at -80° until use.
Tang X., Gao Y., Yu L., Lu Y., Zhou G., Cheng L., Sun K., Zhu B., Xu M, & Liu J. (2017). Correlations between lncRNA-SOX2OT polymorphism and susceptibility to breast cancer in a Chinese population. Biomarkers in medicine, 11(3).
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