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Klh gp61

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KLH-gp61 is a protein that can be used as a carrier molecule in immunological research and applications. It is derived from the hemolymph of the giant keyhole limpet (Megathura crenulata). The core function of KLH-gp61 is to serve as an antigen or hapten carrier to stimulate an immune response.

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Lab products found in correlation

Nunc maxisorp plate, by Thermo Fisher Scientific (2 mentions) Tmb substrate kit, by Thermo Fisher Scientific (2 mentions) Hrp conjugated goat anti mouse igg, by Thermo Fisher Scientific (2 mentions)

2 protocols using klh gp61

1

ELISA for Anti-gp61 Antibody Detection

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Nunc MaxiSorp plates (Thermo Fisher Scientific) were coated overnight at 4 °C with 1 μg/ml KLH-gp61 (Genscript) in PBS. Plates were blocked with PBS + 0.05% Tween-20 + 0.5% BSA for 90 min at 25 °C. After washing, mouse serum was added in a serial dilution in PBS + 0.05% Tween-20 + 0.5% BSA (PBST-B) and incubated for 90 min. After washing, horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Thermofisher Scientific) was added at 1:5,000 in PBST-B for 90 min at 25 °C. Colorimetric detection was performed using a TMB substrate kit (Thermofisher Scientific). Color development was stopped after approximately 5–10 min with 2 N H2SO4, and absorbance was measured at 450 nm.
Choi J., Diao H., Faliti C.E., Truong J., Rossi M., Bélanger S., Yu B., Goldrath A.W., Pipkin M.E, & Crotty S. (2020). Bcl-6 is the nexus transcription factor of T follicular helper cells (TFH) via repressor-of-repressor circuits. Nature immunology, 21(7), 777-789.
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2

ELISA for Anti-gp61 Antibody Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nunc MaxiSorp plates (Thermo Fisher Scientific) were coated overnight at 4 °C with 1 μg/ml KLH-gp61 (Genscript) in PBS. Plates were blocked with PBS + 0.05% Tween-20 + 0.5% BSA for 90 min at 25 °C. After washing, mouse serum was added in a serial dilution in PBS + 0.05% Tween-20 + 0.5% BSA (PBST-B) and incubated for 90 min. After washing, horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Thermofisher Scientific) was added at 1:5,000 in PBST-B for 90 min at 25 °C. Colorimetric detection was performed using a TMB substrate kit (Thermofisher Scientific). Color development was stopped after approximately 5–10 min with 2 N H2SO4, and absorbance was measured at 450 nm.
Choi J., Diao H., Faliti C.E., Truong J., Rossi M., Bélanger S., Yu B., Goldrath A.W., Pipkin M.E, & Crotty S. (2020). Bcl-6 is the nexus transcription factor of T follicular helper cells (TFH) via repressor-of-repressor circuits. Nature immunology, 21(7), 777-789.
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