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66 protocols using 213 portable stadiometer

1

Anthropometric Measurements in Children

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Each child had their height (to the nearest 0.1 cm) and body weight (to the nearest 0.1Kg) measured without shoes and with minimal clothing, using a portable stadiometer (Seca 213 portable stadiometer, Hamburg, Germany) and a digital weighing scale (Seca 869 portable electronic digital weighing scale, Hamburg, Germany) respectively following a standardised procedure. Weight status was calculated as BMI (kilograms per meter squared) and children were categorised as thin/normal weight and overweight/obese using the WHO, 2007 age and gender specific BMI percentiles [57 ].
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2

Ghanaian Infant Growth Monitoring

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As previously described, trained fieldworkers measured infant anthropometrics including weight and length, once within 24 hours of birth23 (link) and at ages three, six, nine and twelve months of age9 (link). Length was measured to the nearest 0.1 centimeter (Ayrton Infantometer Model M-200, Ayrton Corp, MN, USA) and weight was measured to the nearest 0.1 kilogram (Tanita digital scale model BD-590, Tanita Corp, Il, USA). We created Ghanaian-specific birth weight for gestational week curve following methodology described by the World Health Organization24 (link). We considered an infant small for gestational age if the infant was born alive with a birth weight less than the 10th percentile for that specific week gestation.
At the age four lung phenotyping visit, we performed duplicate measures of weight (Seca 803 Clara Digital Floor Scale) to the nearest 0.1 kilogram and height (Seca 213 Portable Stadiometer) to the nearest 0.1 centimeter. We calculated HAZ, WAZ, WHZ at three, six, nine, and twelve months and four years of age using the 2006 WHO child growth standard reference for age and sex25 . Stunting, underweight or wasting was defined as an infant or child with an HAZ, WAZ, or WHZ, respectively, less than two standard deviations below the WHO child growth standard reference median for age and sex.
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3

Anthropometric Measurements Protocol

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Participants wore light clothing and no shoes for anthropometric data collection. Weight was recorded on an electronic digital platform scale (PW-200KGL, Thebarton, Adelaide, Australia) to the nearest 0.1 kg. Height was recorded in duplicate with a stadiometer (213 portable stadiometer; SECA, Hamburg, Germany) to the nearest 0.1 cm. Waist circumference was recorded in duplicate to the nearest 0.1 cm at the mid-point between the lowest rib and the iliac crest using a retractable metal tape (Lufkin W606PM; Cooper Industries, Sparks, NV, USA) according to the International Diabetes Federation Guidelines [44 (link)]. Instruments used for measurements were calibrated and methods validated internally (height and weight) or externally (waist circumference).
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4

Anthropometric Measurements and EEG Data

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World Health Organization (WHO) protocols (The WHO Child Growth Standards, n.d. ) were used to measure the child’s height using the Seca 213 Portable Stadiometer and weight using the SECA-384 electronic scale. Height and weight were used to generate height-for-age (HAZ) and weight-for-age (WAZ) z-scores using WHO growth standards. Head circumference was measured using SECA-201 measuring tape.
Continuous and categorical data were compared using Student’s t-tests and chi-square tests respectively on STATA version 124.
We have uploaded the EDF files, EEG metrics and associated metrics on Brainbase. We are unable to publicly share the.edf files since they are linked to identifiable information. However, this data can be shared with interested parties on a case-by-case basis upon receipt of a reasonable request and exchange of data sharing agreements.
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5

Anthropometric Measurements in Children

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Standing height was measured using a portable stadiometer (Seca 213 Portable Stadiometer, Germany). Body mass and composition (fat percentage, fat mass, fat free mass, bone free lean tissue mass (muscle mass) and total body water) were measured through the bioelectrical impedance analysis (BIA) method (Tanita Body Composition Analyzer (Model BC-420MA)).
Body mass index (BMI) was calculated by dividing the weight in kilograms by height in meters squared. The Z-scores for height for age (HAZ), weight for age (WAZ), BMI for age (BAZ), muscle percentage and fat percentage for age were computed using Indian growth references [21 (link),35 (link)].
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6

Anthropometric Measures in Health Screening

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Participant weight and height were collected at the baseline health screenings, which occurred in the morning. Participants were asked to fast overnight before their appointment. Weight was measured using a calibrated digital Tanita scale (Model: SC-331S). Participants removed shoes, outer clothing (e.g., sweatshirts), belts, and items from their pockets prior to stepping onto the scale. Weight was recorded in kilograms. Participant height, without shoes, was measured using a research grade stadiometer (Seca 213 Portable Stadiometer). We calculated baseline BMI following standardized procedures and categorized BMI scores into obese (BMI ≥ 30) and not obese (BMI < 30). This cut-off reflects literature that suggests higher weight/BMI is associated with poorer HRQoL and that reported HRQoL for overweight individuals does not differ from normal or under- weight adults (Sach et al., 2007 (link), Hassan et al., 2003 (link)).
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7

Maximal Aerobic Capacity and Body Composition Assessment

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A maximal exercise test on a bicycle ergometer (Lode Excalibur, Groningen, The Netherlands) was performed to establish maximal aerobic capacity (VO2max). After an initial workload of 100 Watt for 5 min, workload was subsequently increased by either 25 W/min or 40 W/2 min until the participant could not maintain the required pedaling frequency of at least 60 rpm. Participants were allowed to eat and drink before the test; nothing specific was prescribed. Oxygen consumption was measured with indirect calorimetry (Oxycon Carefusion, Hoechberg, Germany), and VO2 max was recorded [19 (link)]. Heart rate was monitored by using a heart rate monitor (Polar T31-coded, Oulu, Finland) and connected exercise tracker (Polar FT1). In addition, body length (Seca 213 portable stadiometer, Hamburg, Germany) and weight (Seca 761 scale) were measured. Thereafter, DEXA measurements were carried out using a Lunar Prodigy Advanced DEXA scanner (GE Health Care, Madison, WI, USA) [20 (link)]. A quality assurance test was performed to ensure system suitability and precision of the scanner. Whole body scans were performed according to the manufacturer’s protocol and identical scan protocols were used for all subjects.
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8

Comprehensive Anthropometric and Physical Function Assessment

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Body weight was measured using a Seca 899 digital scale to the nearest 0.1 kg, with participants wearing light clothing and no shoes. Height was measured using a Seca 213 portable stadiometer to the nearest 0.1 cm, with participants wearing no shoes/minimal headwear. Body Mass Index (BMI) was calculated using the standard equation, weight (kg)/height (m)2. Waist circumference was measured using a flexible retractable non-elastic tape and recorded to the nearest 0.01 cm.
The Short Physical Performance Battery (SPPB) was used to measure participants’ lower body extremity physical function [50 (link)–52 (link)]. These measures include gait speed, balance and time taken to perform five repeated chair rises [52 (link)]. Handgrip strength was measured using a Jamar hand dynamometer in the standing position [53 ]. The standing position was chosen as it has been shown to produce maximal grip strength as compared to other body positions [54 ]. During all measurements, the Jamar handgrip dynamometer was adjusted to the second handle as this has also been shown to produce consistent and reliable maximal handgrip strength results [55 (link)]. Each measurement was completed three times and the highest recording used for analysis.
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9

Anthropometric Measurements Protocol

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Body measurements included height (measured using a Seca 213 portable
stadiometer), weight, blood pressure, and WC. With the participants in light
clothing and without shoes, the WC was measured midway between the lower rib
margin and the iliac-crest at the end of gentle expiration in the standing
position using portable stadiometer. BMI was calculated by dividing weight (kg)
by height squared (m2) (Janghorbani & Amini, 2016 (link)).
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10

Comprehensive Dietary and Physical Activity Assessment

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Participants provided demographic information, including age and occupation, and were asked to complete a 3-day food diary and the Global Physical Activity Questionnaire (GPAQ), as described below. Quantities of foods and beverages were either estimated in household measures or weighed using kitchen scales, as per the preference of the participant. Instructions on measuring different foods and beverages were also provided to participants by the study dietitian and were included as part of the food diary. After completing the above, participants met with the study dietitian who ensured that the food diary was completed correctly, including extra details as required during the interview. Participant’s weight and height were measured at this time, in light clothing and without shoes, using an electronic digital platform scale (PW-200KGL, Thebarton, Adelaide, Australia) to the nearest 0.01 kg and a stadiometer (213 portable stadiometer; SECA, Hamburg, Germany) to the nearest 0.1 cm, respectively.
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