Tadalafil

Manufactured by Selleck Chemicals

Sourced in United States

Tadalafil is a white to off-white crystalline solid that is practically insoluble in water. It is a phosphodiesterase type 5 (PDE5) inhibitor. Tadalafil is used as a reference standard in analytical procedures.

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Lab products found in correlation

5 protocols using tadalafil

Tadalafil Inhibits PDE5 In Vitro and In Vivo

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Tadalafil (Selleckchem, TX, USA), a phosphodiesterase-5 (PDE5) inhibitor, was used in vitro at a concentration of 100 μM and was administered by intraperitoneal (i.p.) injection (2 mg/kg/24hr) in vivo.[22 (link)] N-omega-hydroxy-L-arginine (Sigma-Aldrich, MO, USA) and N(G)-monomethyl-L-arginine (Sigma-Aldrich, MO, USA) were added to cell cultures (10 μM in vitro.
The methods of cell isolation, generation of CIK cells, isolation of MDSCs, flow cytometry analysis, cytotoxicity assays, cytokine array and quantitative real-time PCR are described in the Supporting information.

Yu S.J., Ma C., Heinrich B., Brown Z.J., Sandhu M., Zhang Q., Fu Q., Agdashian D., Rosato U., Korangy F, & Greten T.F. (2018). Targeting the crosstalk between cytokine-induced killer cells and myeloid-derived suppressor cells in hepatocellular carcinoma. Journal of hepatology, 70(3), 449-457.

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Evaluation of Nitric Oxide Signaling

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The NO donors S-Nitroso-N-Acetyl-D,L-Penicillamine (SNAP) and (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (DETA NONOate) were obtained from Cayman Chemical (Ann Arbor, MI), and sodium nitroprusside (SNP) was obtained from Sigma Aldrich (St. Louis, MO). The sGC stimulators BAY 41-2272 (BAY) and YC-1, and the sGC inhibitor 1H-[1, 2, 4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) were from Cayman. The PDE5 inhibitors Tadalafil (Cialis) and Sildenafil (Viagra) were from Selleck Chemical (Houston, TX). The PKG inhibitor KT5823 was from Cayman.

Tuttle T.R., Mierzwa M.L., Wells S.I., Fox S.R, & Ben-Jonathan N. (2015). The cyclic GMP/protein kinase G pathway as a therapeutic target in head and neck squamous cell carcinoma. Cancer letters, 370(2), 279-285.

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Wnt/β-Catenin Signaling Pathway Analysis

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Tadalafil and vardenafil were obtained from Selleckchem (Houston, TX, USA).
KT5823, 8-Br(Bromo)-cGMP, Alizarin Red S, and SB216763 were from Sigma (St Louis,
MO, USA). Recombinant murine Wnt3a protein was purchased from R&D System
(Minneapolis, MN, USA). Antibodies against PKG1, PKG2, Lamin B, Dkk1, IgG,
β-actin, and protein A/G PLUS-Agarose were from Santa Cruz
Biotechnology (Santa Cruz, CA, USA). Antibodies against p-β-cat
(Ser33/37/Thr41), p-β-cat (Ser45), β-cat,
p-GSK3β (Ser9) and GSK3β were from Cell Signaling
(Danvers, MA, USA). Antibodies against AP and RunX2 were purchased from Abcam
(Cambridge, UK), and the antibody against Lef1 was from Proteintech (Chicago, IL,
USA). The IRDye 680 and 800 second antibodies were from LI-COR Bioscience
(Lincoln, Nebraska) and the Alexa 555-conjugated secondary antibodies were from
Life Technologies (Grand Island, NY, USA). Cyclic GMP Direct Immunoassay kits were
obtained from Abcam and TRAP staining kits were from Sigma.

Gong Y., Xu C.Y., Wang J.R., Hu X.H., Hong D., Ji X., Shi W., Chen H.X., Wang H.B, & Wu X.M. (2014). Inhibition of phosphodiesterase 5 reduces bone mass by suppression of canonical Wnt signaling. Cell Death & Disease, 5(11), e1544-.

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Zebrafish Inhibitor Treatment Protocols

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Sildenafil citrate (Pfizer), vardenafil HCl trihydrate (SelleckChem, Houston, TX, USA), and tadalafil (SelleckChem) were dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich Corp., St. Louis, MO, USA) at 100 μM for stock concentration. Working concentrations were diluted further in DMSO prior to addition to fish water such that final DMSO concentration was 0.05% for all experiments except the sildenafil dilution series, which used 0.1%. Larval fish were treated with inhibitor for 3 days. Adult fish were treated with inhibitor for 6 days with a change of water and fresh inhibitor after 3 days.

Campbell L.J, & Jensen A.M. (2017). Phosphodiesterase Inhibitors Sildenafil and Vardenafil Reduce Zebrafish Rod Photoreceptor Outer Segment Shedding. Investigative Ophthalmology & Visual Science, 58(13), 5604-5615.

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Midazolam Metabolism and CYP3A Inhibition

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Midazolam was purchased from Wako Pure Chemical Industries (Osaka, Japan). The metabolite of Midazolam, 19-hydroxyMidazolam, was purchased from Sigma-Aldrich (St. Louis, MO). As an internal standard, 13 C-19-hydroxyMidazolam was obtained from Corning (Corning, NY). The following marketed drugs were used for the evaluation of the potential to inhibit or induce CYP3A: atomoxetine hydrochloride (Tokyo Chemical Industry, Tokyo, Japan), atorvastatin (LKT Laboratories, St. Paul, MN), azithromycin dehydrate (LKT Laboratories), casopitant mesylate (Santa Cruz Biotechnology, Dallas, TX), cimetidine (Sigma-Aldrich), deferasirox (Toronto Research Chemicals, North York, Canada), ethinyl estradiol (Sigma-Aldrich), everolimus (Selleck Chemicals, Houston, TX), felodipine (Sigma-Aldrich), fluoxetine (Sigma-Aldrich), fluvoxamine (Sigma-Aldrich), pazopanib (ChemieTek, Indianapolis, IN), ranitidine hydrochloride (Sigma-Aldrich), roxithromycin (Sigma-Aldrich), simvastatin (Wako Pure Chemical), suvorexant (AdooQ-BioScience, Irvine, CA), and tadalafil (Selleck Chemicals). Pooled human microsomes (mixed sex, 20 mg protein/ml) and pooled cryopreserved human hepatocytes were purchased from Sekisui XenoTech, LLC (Kansas City, KS). NADPH Regeneration System Solution A and NADPH Regeneration System Solution B were purchased from Corning.

Yamada M., Inoue S.I., Sugiyama D., Nishiya Y., Ishizuka T., Watanabe A., Watanabe K., Yamashita S, & Watanabe N. (2020). Critical Impact of Drug-Drug Interactions via Intestinal CYP3A in the Risk Assessment of Weak Perpetrators Using Physiologically Based Pharmacokinetic Models. Drug metabolism and disposition: the biological fate of chemicals, 48(4).

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