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Mir 9 5p mimic

Manufactured by RiboBio
Sourced in China

The MiR-9-5p mimic is a synthetic RNA molecule designed to mimic the function of the naturally occurring microRNA miR-9-5p. microRNAs are small non-coding RNA molecules that play important regulatory roles in gene expression.

Automatically generated - may contain errors

3 protocols using mir 9 5p mimic

1

Transfection of miR-9-5p in Mouse Microglia

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miR-9-5p mimic control (miR-Con), miR-9-5p mimic (miR-9), miR-9-5p inhibitor control (anti-miR-Con), and miR-9-5p inhibitor (anti-miR-9) were synthesized by Ribobio (Guangzhou, China). Primary mouse microglia and BV-2 cells were seeded at 1 × 105 cells per well in a 24-well plate in 1 ml of antibiotic-free standard growth medium and grown to 40–80% confluency. Then, cells were transfected with 50 nM miR-Con or miR-9 or with 100 nM anti-miR-Con or anti-miR-9. 1 μl RiboFECT™CP reagent (RiboBio, Guangzhou, China) was added to each well according to the manufacturer’s protocols. The medium containing RiboFECT™CP reagent was replaced after 24 h of transfection. Transfection efficiency was determined via western blotting.
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2

Luciferase Reporter Vectors for miRNA Regulation

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MiRNA scramble, miR-331-3p mimic, miR-331-3p inhibitor, miR-331-3p antagomir, miR-9-5p mimic, miR-9-5p inhibitor, miR-9-5p antagomir were obtained from RIBOBIO Corporation (Guangzhou, China). To construct luciferase reporter vectors, we cloned the approximately 400bp 3'UTR sequences (extended 200bp on both sides of the microRNA targeting sequences) into pmirGLO between NheI and XbaI. Mutated vectors were constructed into the same site changing the seed sequences of microRNA from G to A, C to T. The sequences were synthesized in GenePharma (Shanghai, China).
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3

Modulating circRNA 0090231 in HUVECs

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For circ_0090231 stable expression, HUVECs were infected with sh-circ_0090231, oe-circ_0090231, and their controls (sh-NC or vector), followed by selecting with puromycin. And lentiviral vectors were acquired from Genechem (Shanghai, China). Meanwhile, 30 nM of miR-9-5p mimic, miR-9-5p inhibitor, miR-NC mimic, miR-NC inhibitor, and 200 ng of pcDNA-TXNIP (TXNIP) and pcDNA from RiboBio (Guangzhou, China) were respectively transfected into HUVECs, according to the instruction guidelines of Lipofectamine 3000 (Invitrogen).
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