Anti mouse and anti rabbit igg horseradish peroxidase conjugated secondary antibodies

Neferine was isolated according to the previous method [6 (link)]. Temozolomide was obtained from Orion Corporation (New Jersey, USA). The primary antibodies of FAK, p-FAK, S6K, p-S6K, Beclin-1, LC3, Cleaved Caspase-3, and PARP were purchased from Cell Signaling (Danvers, MA, USA). The antibody for β-actin was obtained from Sigma (Kawasaki, Japan), the anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies were purchased from GE Healthcare (Chicago, IL, USA).

The primary antibodies for Bak, pFAK, Bad, Bax, Bcl2, and PARP were purchased from Cell Signaling (Danvers, MA, USA). Rac1 and Cdc42 antibodies were obtained from Millipore (Billerica, MA, USA). The FAK antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA), p53 and NF-kB antibodies were purchased from Neomarkers (Fremont, CA, USA), the vimentin and E-cadherin antibody were obtained from BD Biosciences (CA, USA), and β-catenin, Smad 2, and 3 antibodies were obtained from Genetex (Irvine, CA, USA). The antibody for β-actin was obtained from Sigma (USA), the anti-mouse and anti-rabbit IgG horseradish peroxidase-conjugated secondary antibodies were purchased from GE Healthcare (UK). PF-431396 was purchased from Sigma Aldrich. Synthetic antroquinonol [24 (link)] was obtained from Dr. Chin Po Chen (Department of Chemistry, NDHU, Hualien, Taiwan).