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2 protocols using nunclon 96 well flat bottom black microwell plates

1

Synthesis of Antibacterial Silica Nanoparticles

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All reagents were of analytical or HPLC grade and were used as received. Aminopropyltriethoxysilane (APTES), Tetraethoxysilane (TEOS), Ammonium hydroxide solution (28%–30%), hydrogen peroxide (H2O2, 30%), Gentamicin (GM) and amoxicillin (AM) and tetracycline (TC) were obtained from Sigma Aldrich. Nunclon 96-well flat-bottom black microwell plates were purchased from Thermo scientific, DK. 0.1% milk (Arla) was bought from a local supplier. The water used in the experiment was obtained from a Millipore (MilliQ) purification system.
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2

Functionalized Polymer Hydrogel Synthesis

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Methacrylic acid (MAA), ethylene glycol dimethacrylate (EGDMA), trimethylolpropane trimethacrylate (TRIM), pentaerythritol-tetrakis-(3-mercaptopropionate) (PETMP), phosphate buffer solution (PBS) (pH 7.4), sodium hydroxide, glutaraldehyde (GA), histamine, histidine, melamine, (3-aminopropyl)triethoxysilane (APTES). Carbodiimide (EDC), N-hydroxysuccinimide (NHS) 98%, ethanolamine hydrochloride, hydrochloride acid (HCl), 11-mercaptoundecanoic acid (MUDA) 95%, hydrogen peroxide, acetonitrile (ACN), ethanol, acetone, phthaldialdehyde, 60 mL SPE tubes and 20 μm pore frits and glass beads (75 μm), were purchased from Sigma-Aldrich (DK). 2,4,6-Trinitrobenzene sulfonic acid (TNBSA) was purchased from Thermofisher Scientific (DK). N,N-Diethyldithiocarbamic acid benzyl ester (DABE) was obtained from TCI Europe (Belgium). Q-Max® Celluloseacetat (CA) syringe filters 0.45 μm were from FRISENETTE (DK). Gold chip was purchased from Bionavis, Sweden. Nunclon 96-well flat-bottom black microwell plates were purchased from Thermo scientific, DK. 0.1% milk (Arla) was bought from a local supermarket, Netto. Deionized water was obtained from a Millipore (MilliQ) purification system. All chemicals were analytical or HPLC grade and were used without further purification.
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