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Si nc

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The Si-NC is a lab equipment product designed for research and scientific applications. It serves as a core function to facilitate analysis and experimentation within a controlled laboratory environment.

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Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (21 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (15 mentions) Mir nc, by Sangon (9 mentions) Si nc, by Thermo Fisher Scientific (8 mentions) Anti mir nc, by Sangon (6 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (5 mentions) Mimic nc, by Sangon (5 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (5 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Inhibitor nc, by Sangon (4 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (4 mentions) Si malat1, by Sangon (3 mentions) Lipofectamine 2000, by Sangon (3 mentions) Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (3 mentions) Nc mimic, by Thermo Fisher Scientific (2 mentions) Pcdna3, by Sangon (2 mentions) Si meg3, by Sangon (2 mentions) Sh nc, by Sangon (2 mentions) Pcdna3.1 vector, by Sangon (2 mentions) Si dnmt3a, by Sangon (2 mentions)

Spelling variants of this product

Si-normal control (si-NC) (2 citations)

76 protocols using si nc

1

Modulating MALAT1 Expression in Cell Lines

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Cell transfection was performed before cultured cells were grouped into four groups: pcDNA3.1 (empty vector pcDNA3.1), pcDNA3.1-MALAT1 (to induce MALAT1 overexpression), si-MALAT1 (to induce MALAT1 knockdown), and si-NC (control for MALAT1 knockdown).
The following sequence of si-RNA oligonucleotides (si-MALAT1) was used to knockdown MALAT1 expression: 5ʹ-CACAGGGAAAGCGAGUGGUUGGUA-3ʹ. The sequence of the noncoding control siRNA (si-NC) was 5ʹ-UUCUCCGAACGUGUCACGU-3ʹ. si-NC and si-MALAT1 were synthesized by Shanghai Sangon Biotechnology Co., Ltd. (Shanghai, China). pcDNA3.1 and pcDNA3.1-MALAT1 were purchased from Shanghai GenePharma Co., Ltd. (Shanghai, China).Cell transfection was performed by introducing 100 nM of miR-503-5p mimic, 100 nM of miR-503-5p inhibitor, mimics negative control (NC), inhibitor negative control, si-MALAT1, si-NC, pcDNA3.1, or pcDNA3.1-MALAT1 into cells for incubation at 37°C in a humidified chamber with 5% CO2 for correspondently 24, 48, and 72 hrs. Lipofectamine® 2000 transfection reagent (Invitrogen; Thermo Fischer Scientific, Inc.) was used according to the manufacturer’s protocol. After transfection for 48 hrs, RT-qPCR was performed to assess the transfection efficiency of MALAT1 knockdown and overexpression.
Sun Q., Li Q, & Xie F. (2019). LncRNA-MALAT1 regulates proliferation and apoptosis of ovarian cancer cells by targeting miR-503-5p. OncoTargets and therapy, 12, 6297-6307.
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2

Generation and Evaluation of modified siRNA Targeting HBV Genes

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The sequences of siHBs and siHBx were obtained from a previous study [18] (link). msiRNAs were generated by substituting the 9-12th nucleotides of the passenger strand of siRNAs with unpaired uracil nucleotides (Fig. 1). msiRNAs targeting the HBV S and X genes with the same target sequences as siHBs and siHBx were designed and named msiHBs and msiHBx, respectively. siNC was purchased from Sangon Biotech (Shanghai, China) and used as a negative control. All siRNAs were synthesized by Sangon Biotech (Shanghai, China). The HBV replication-competent plasmid pHY106-wta (1.3 copies of HBV generated from pSM2 [19] (link)) was a kind gift from Prof. Mengji Lu at Essen University Hospital, Germany. The HBV replication-competent plasmid pHY106-X15 was constructed by cloning a clinically derived single copy of the HBV sequence (genotype C, GenBank accession No. KM213037) into the pHY106 backbone.

The sequences and structures of siRNAs and msiRNAs with unpaired uracil bulges. The uracil substitution at the 9–12th nucleotides of the passenger strand of siRNAs is indicated in red. siHBs, siRNA targeting the HBV S gene. siHBx, siRNA targeting the HBV X gene. msiHBs, msiRNA targeting the HBV S gene. msiHBx, msiRNA targeting the HBV X gene. siNC was purchased from Sangon Biotech (Shanghai, China) and used as a negative control

Lan T., Wei Z., He Y., Wan S., Liu L., Cheng B., Li R., Chen H., Liu G, & Meng Z. (2021). Immunostimulatory siRNA with a uridine bulge leads to potent inhibition of HBV and activation of innate immunity. Virology Journal, 18, 37.
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3

Knockdown of ErbB4 in vitro and in vivo

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In vitro, to knock down the expression of human ErbB4, siRNA for ErbB4 (si-ErbB4: 5'-ATGCCATTTGGACA-TGTAATTGT-3') was utilized and non-specific control (si-NC: 5'-CCGUUGAAAGGCCUACCCUCA-3') were purchased from Sangon Biotech. All these sequences were transfected onto cells that grew to 60% confluence with INTERFERin (Polyplus, France). After 36 h cultured at 37˚C, 5% CO 2 , cells were collected after transfection.
In vivo, to knock down the expression of mouse ErbB4, siRNA for ErbB4 (si-ErbB4: 5'-CGGGAACTAGCTG-TACGTTGTGC-3') was utilized and non-specific control (si-NC: 5'-GCUUGCGGACCAUCGAGT-3') were purchased from Sangon Biotech. All these sequences were transfected onto mice by in-vivo-JETRNA (Polyplus, France).
Tang R., Cui H., Dou R., Tao L., Tan L., Guo T, & Luo D. (2023). ErbB4 affects Th1/Th17 cell differentiation and promotes psoriasis progression. Cellular and molecular biology (Noisy-le-Grand, France), 69(12).
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4

Modulating PVT1 and miR-15a-5p in Prostate Cancer

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For PVT1 downregulation, small interference RNA (siRNA) against PVT1 (si-PVT1) and its negative control (si-NC) were assembled by Sangon Biotech (Shanghai, China). For stable PVT1 knockdown, lentiviral vector (lenti-short hairpin sh-PVT1) and its negative control (sh-NC) were obtained from Genechem (Shanghai, China). For miR-15a-5p inhibition or enrichment, miR-15a-5p inhibitor (5′-CACAAACCAUUAUGUGCUGCUA-3′) or miR-15a-5p mimic (sense: 5′-UAGCAGCACAUAAUGGUUUGUG-3′ and antisense: 5′-CAAACCAUUAUGUGCUGCUAUU-3′) and negative control (inhibitor NC (5′-CAGUACUUUUGUGUAGUACAA-3′) or miR-NC (sense: 5′-UUCUCCGAACGUGUCACGUTT-3′ and antisense: 5′-ACGUGACACGUUCGGAGAATT-3′)) were purchased from Ribobio (Guangzhou, China). For KIF23 knockdown, siRNA against KIF23 (si-KIF23) and si-NC were also constructed by Sangon Biotech. The 22RV1 and DU145 cells were subjected to transfection with above items using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Following experiments were conducted at 48 h post-transfection.
Wu H., Tian X, & Zhu C. (2020). Knockdown of lncRNA PVT1 inhibits prostate cancer progression in vitro and in vivo by the suppression of KIF23 through stimulating miR-15a-5p. Cancer Cell International, 20, 283.
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5

Modulation of miR-21 and VHL in Lymphoma

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The modified miR-21 mimics and the control (NC mimics); miR-21 inhibitor (anti-miR-21) and negative control (anti-NC); and siRNA targeting VHL (si-VHL) and corresponding control (si-NC) were all designed and synthesized by Sangon Biotech (Shanghai, China). 50 nM of the indicated oligonucleotide was transiently transfected into cells by Lipofectamine™ 2000 Transfection Reagent (Invitrogen, Breda, the Netherlands) according to the directions of manufacturers.
For curcumin treatment, SU-DHL-8 cells were treated with different concentrations (0, 5, 10, 20, 40, and 60 µmol/L) of curcumin (Sigma-Aldrich, St. Louis, MO, USA), or transfected cells were treated with 20 µmol/L of curcumin, followed by the detection of cell proliferation, migration, invasion, and apoptosis capacities.
Chen L., Zhan C.Z., Wang T., You H, & Yao R. (2019). Curcumin Inhibits the Proliferation, Migration, Invasion, and Apoptosis of Diffuse Large B-Cell Lymphoma Cell Line by Regulating MiR-21/VHL Axis. Yonsei Medical Journal, 61(1), 20-29.
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6

Transfection of si-lncRNA Dlx6os1, si-EZH2, and si-SOX6 in SV40 MES13 cells

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The transfection doses for si-lncRNA Dlx6os1, si-EZH2, and si-SOX6 and its negative control si-NC (synthesized by Sangon Biotech, Shanghai, China) were 2 μg/well for SV40 MES13 cells in 6-well plates. All transfections were performed using Liposome TM 3000 transfection reagent (Kusatsu Takayama, Japan). After transfection for 48 h, follow-up experiments were carried out with SV40 MES13 cells.
Chen Y.X., Zhu S.Y., Huang C., Xu C.Y., Fang X.D, & Tu W.P. (2022). LncRNA Dlx6os1 Accelerates Diabetic Nephropathy Progression by Epigenetically Repressing SOX6 via Recruiting EZH2. Kidney & blood pressure research, 47(3).
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7

Ezrin Silencing in Prostate Cancer Cells

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The human prostate cancer cell lines 22RV1 and PC-3 were cultured in RPMI‑1640 medium (Corning, USA) containing 10% fetal bovine serum (FBS; Life Technologies, USA) supplemented with 100 units/ml streptomycin and penicillin at 37°C in a 5% CO2 atmosphere. Cells were subcultured using 0.25% trypsin/EDTA solution (Invitrogen, USA) every 2 ~ 3 days.
22RV1 and PC-3 cells were added to 6-well plates and incubated for 24 h at 37°C at 5 × 105 cells/well. Then, cells were transfected with blank, negative control (NC), si-NC, Ezrin-overexpression and si-Ezrin plasmids (Sangon Biotech, China) using Lipofectamine 2000 (Promega, USA).
Chen Z., Wang J., Lu Y., Lai C., Qu L, & Zhuo Y. (2022). Ezrin expression in circulating tumor cells is a predictor of prostate cancer metastasis. Bioengineered, 13(2), 4076-4084.
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8

Silencing CCAT2 in Thyroid Cancer Cells

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Since TPC-1 and FTC-133 cells exhibited a higher CCAT2 expression compared with the other TC cells, they were used for subsequent studies. To silence CCAT2 expression in TC cells, a specific small interfering (si)RNA against CCAT2 (si-CCAT2) and a negative control (si-NC) were purchased from Sangon Biotechnology Co., Ltd. After culturing the cells in 24-well plates at 37°C for 24 h, the TC cells (1×105 cells/well) were transfected with si-CCAT2 (50 nmol) or si-NC (50 nmol) using Lipofectamine 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. Following transfection for ≥24 h, the CCAT2-silenced TC cells were used for further study. Knockdown efficiency of si-CCAT2 in TC cells was detected by RT-qPCR. The sequences used were as follows: si-CCAT2 sense, 5′-AAG UCC ACC UGA UCA CCU CGG-3′ and antisense, 5′-GAG GUG AUC AGG UGG ACU UUC-3′; and si-NC sense, 5′-AUA AGU CAC CUC CAC CUG CGG-3′ and antisense, 5′-GCU UGA GAA GGG UGA UCU GUC-3′.
Xin S, & Ye X. (2020). Knockdown of long non-coding RNA CCAT2 suppresses the progression of thyroid cancer by inhibiting the Wnt/β-catenin pathway. International Journal of Molecular Medicine, 46(6), 2047-2056.
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9

Targeting AMRc8 in Bladder Cancer

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The small interfering RNA against AMRc8 (si-AMRc8) and its negative control siRNA (si-NC) were obtained from Shanghai Sangon Co. Ltd (Shanghai, P.R. China). For in vitro transfection, si-AMRc8 or si-NC was transfected into bladder cancer cells using Lipofectamine 2000 transfection reagent (Invitrogen) according to the manufacturer’s instructions. siRNA knockdown efficiency was verified by Western blot detection of AMRc8 expression.
Liang X., Men Q.L., Li Y.W., Li H.C., Chong T, & Li Z.L. (2017). Silencing of Armadillo Repeat-Containing Protein 8 (ARMc8) Inhibits TGF-β-Induced EMT in Bladder Carcinoma UMUC3 Cells. Oncology Research, 25(1), 99-105.
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10

Transfection Protocols for FENDRR and miR-423-5p Studies in HUVECs

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HUVECs were purchased from Biobw Biotechnology Co. (Beijing, China) and cultured in DMEM medium plus 10% FBS and 1% mixed antibiotics. The humidity of incubation was maintained at 70–80% and 5% CO2 at 37°C.
The si-FENDRR and its negative control (si-NC) together with miR-423-5p mimics, miR-423-5p inhibitors, and their negative controls (mimic-NC and inhibitor-NC) were purchased from Sangon Biotech (Shanghai, China). All these sequences used in the transfection were provided in Table 1. The transfection experiments were put into practice using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).

Sequences Used for Transfection

NameSequences
si-FENDRR5’-GGAGGGAATTAGAAGCGTT-3’
si-NC5’-TTCTCCGAACGTGTCACGT-3’
miR-423-5p mimic5’-UGAGGGGCAGAGAGCGAGACUUU-3’
miR-423-5p inhibitor5’-ACTCCCCGTCTCTCGCTCTGAAA-3’
mimic-NC5’-UUCUCCGAACGUGUCACGUTT-3’
inhibitor-NC5’-UCACAACCUCCUAGAAAGAGUAGA-3’

Abbreviation: NC, negative control.

Zhao X., Wang C., Liu M., Meng F, & Liu K. (2022). LncRNA FENDRR Servers as a Possible Marker of Essential Hypertension and Regulates Human Umbilical Vein Endothelial Cells Dysfunction via miR-423-5p/Nox4 Axis. International Journal of General Medicine, 15, 2529-2540.
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