CP specimens were collected at different time points. To ensure collection of sufficient amount of normal CP specimens, each normal CP specimen includes tissues pooled from 2 wild type animals for P7, P14 and P22. Information on animals used is available in Supplementary Table 9 . The investigator was blinded to group allocation. Tissues were fixed in 4% PFA, 1% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.4) at 4°C overnight. After washing with cacodylate buffer supplemented with 10% sucrose, tissues were post-fixed with 1% osmium tetroxide (OsO4), followed by incubation with 1% uranyl acetate in 30% ethanol. Tissue samples were dehydrated, then transferred to propylene oxide, and embedded in Eponate-12 epoxy resin (Ted Pella, Redding, CA). Tissue samples were sectioned (85 nm thickness) with a Leica UC-6 ultramicrotome (Wein, Austria). Sections were counterstained with uranyl acetate and lead citrate, and observed under the JEOL 1400 transmission electron microscope (JEOL Ltd, Tokyo, Japan). Images were taken with a Gatan UltraScan 1000 CCD digital camera (Gatan, Inc., Pleasanton, CA).
Ultrascan 1000 ccd digital camera
Manufactured by Ametek
The UltraScan 1000 CCD digital camera is a compact, high-resolution camera designed for laboratory applications. It features a large-format CCD sensor that captures detailed images with a resolution of up to 4096 x 4096 pixels. The camera's core function is to provide researchers and scientists with a versatile imaging tool for a wide range of scientific and analytical applications.
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2 protocols using ultrascan 1000 ccd digital camera
1
Ultrastructural Analysis of Choroid Plexus
2
Ultrastructural Analysis of Choroid Plexus
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CP specimens were collected at different time points. To ensure collection of sufficient amount of normal CP specimens, each normal CP specimen includes tissues pooled from 2 wild type animals for P7, P14 and P22. Information on animals used is available in Supplementary Table 9 . The investigator was blinded to group allocation. Tissues were fixed in 4% PFA, 1% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.4) at 4°C overnight. After washing with cacodylate buffer supplemented with 10% sucrose, tissues were post-fixed with 1% osmium tetroxide (OsO4), followed by incubation with 1% uranyl acetate in 30% ethanol. Tissue samples were dehydrated, then transferred to propylene oxide, and embedded in Eponate-12 epoxy resin (Ted Pella, Redding, CA). Tissue samples were sectioned (85 nm thickness) with a Leica UC-6 ultramicrotome (Wein, Austria). Sections were counterstained with uranyl acetate and lead citrate, and observed under the JEOL 1400 transmission electron microscope (JEOL Ltd, Tokyo, Japan). Images were taken with a Gatan UltraScan 1000 CCD digital camera (Gatan, Inc., Pleasanton, CA).
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