Sodium sulfide na2s

Manufactured by Merck Group

Sourced in United States, Macao, Spain

Sodium sulfide (Na2S) is an inorganic compound that is a crystalline solid at room temperature. It is a source of the sulfide ion (S2-) and is used in various industrial and laboratory applications.

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7 protocols using sodium sulfide na2s

Synthesis of Colloidal Nanoparticles

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Graphite power (<20 μm, synthetic), potassium permanganate (KMnO₄, ≥99%), potassium persulfate (K₂S₂O₈, ≥99%), potassium dihydrogen phosphate (K₂HPO₄, ≥99.999%), phosphorus pentoxide (P₂O₅, ≥98%), Sulfuric acid (H₂SO₄, 95-97%), hydrazine hydrate solution (50-60%), ammonia solution (25%), toluene (99.9%), Cadmium oxide (CdO, 99.5%), selenium (Se,>99.5%), oleic acid (OA,>99%), octadecane (ODE, ≥95%), trioctylphospine (TOP,>90%), Sodium sulfide (Na₂S) and Sulfur (S, ≥99.5%) were all from Sigma-Aldrich and used as received. Milli-Q water (18.2 MΩ cm) was used throughout.

Zhu N., Zheng K., Karki K.J., Abdellah M., Zhu Q., Carlson S., Haase D., Žídek K., Ulstrup J., Canton S.E., Pullerits T, & Chi Q. (2015). Sandwiched confinement of quantum dots in graphene matrix for efficient electron transfer and photocurrent production. Scientific Reports, 5, 9860.

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Multifunctional Nanoparticle Theranostics

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Indocyanine green (ICG), poly(D,L-lactide-co-glycolide) (PLGA, MW, 5000–15000; lactide, glycolide (50:50)), hematoxylin and eosin, Sodium sulfide (Na₂S), and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were purchased from Sigma-Aldrich (USA). Bovine hemoglobin (Hb) was purchased from J&K Scientific Ltd. (China). Soybean lecithin, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-maleimide (polyethylene glycol 2000) (DSPE-PEG) were obtained from Avanti (USA). Coomassie Blue, Calcein-AM and Propidium Iodide (PI) Cell Apoptosis Kit were obtained from Invitrogen (USA). Fetal bovine serum, dulbecco’s modified eagle medium (DMEM) and penicillin-streptomycin were purchased from Gibco Life Technologies (USA). 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H- tetrazolium (MTS) was bought from Promega (USA). Amicon ultra-4 centrifugal filter with a molecular weight cutoff of 100 kDa was bought from Millipore (USA).

Luo Z., Zheng M., Zhao P., Chen Z., Siu F., Gong P., Gao G., Sheng Z., Zheng C., Ma Y, & Cai L. (2016). Self-Monitoring Artificial Red Cells with Sufficient Oxygen Supply for Enhanced Photodynamic Therapy. Scientific Reports, 6, 23393.

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Synthesis of Zinc Sulfide Nanoparticles

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Zinc
nitrate hexahydrate
(Zn(NO₃)₂·6H₂O, Sigma-Aldrich),
Poly(ethylenimine) (PEI) solution (Sigma-Aldrich, MW ∼750,000
in H₂O), nitric acid (HNO₃, Sigma-Aldrich),
ammonium hydroxide solution (NH₄OH, Sigma-Aldrich), sodium
sulfide (Na₂S, Sigma-Aldrich), an urea (CH₄N₂O, Sigma-Aldrich) were purchased and used in the experiments.

Tuc Altaf C., Colak T.O., Rostas A.M., Popa A., Toloman D., Suciu M., Demirci Sankir N, & Sankir M. (2023). Impact on the Photocatalytic Dye Degradation of Morphology and Annealing-Induced Defects in Zinc Oxide Nanostructures. ACS Omega, 8(17), 14952-14964.

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Synthesis and Characterization of Ge-132 Compound

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Poly-trans-[(2-carboxyethyl)germasesquioxane] (Ge-132), was synthesized in Asai Germanium Research Institute Co., Ltd. (Kawasaki, Japan). Cyclophosphamide (CPA) and sodium sulfide (Na₂S) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Zinc chloride (ZnCl₂), sodium hydrogen sulfide (NaSH), TTA-A2 and cerulein were purchased from Kishida Chemicals Co., Ltd. (Osaka, Japan), Strem Chemicals, Inc. (Newburyport, MA, USA), Alomone Labs. (Jerusalem, Israel) and Bachem (Bubendorf, Switzerland), respectively. THGP, NaSH and Na₂S were dissolved in ultra-pure water for the MS/MS and whole-cell patch clamp experiments, and in phosphate buffer (20 mM, pH 7.4) or Tris-HCl buffer (1 M, pH 7.4) prepared with D₂O instead of H₂O for ¹H NMR analysis. For administration to mice, THGP, NaSH, Na₂S, CPA and cerulein were dissolved in saline, and TTA-A2 was in 0.5% methyl cellulose containing 1.3% DMSO and 10% Tween-80.

Sekiguchi F., Koike N., Shimada Y., Sugimoto K., Masuda H., Nakamura T., Yamaguchi H., Tanabe G., Marumoto S., Kasanami Y., Tsubota M., Ohkubo T., Yoshida S, & Kawabata A. (2022). A hydrolysate of poly-trans-[(2-carboxyethyl)germasesquioxane] (Ge-132) suppresses Cav3.2-dependent pain by sequestering exogenous and endogenous sulfide. Redox Biology, 59, 102579.

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Cardiac Apoptosis Assay Using Sodium Sulfide

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Sodium sulfide (Na₂S) was purchased from Sigma-Aldrich (St. Louis, MO). Primary antibodies against Cofilin-2, Bcl-2, Bax and galectin-3 were purchased from Santa Cruz Biotechnologies (Dallas, TX). Anti GAPDH was purchased from Cell Signaling Technology (Danvers, MA). Anti-Apoptosis Speck-like protein (ASC) and anti-Cardiac actin were purchased from Sigma-Aldrich. Secondary antibody Anti-rabbit Alexa Fluor 594 and 488–conjugated, 4′,6-diamidino-2-phenylindole (DAPI) and Slow Antifade were purchased from Invitrogen (Waltham, MA). Caspase-1 fluorogenic substrate Ac-Tyr-Val-Ala-Asp-7-amino-4-methyl coumarin (Ac-YVAD-AMC) was purchased from Enzo Life Sciences (Farmingdale, NY). Radio-immunoprecipitation assay (RIPA) buffer was purchased from Cell Signaling Technology. Masson’s trichrome staining kit was purchased from Sigma-Aldrich.

Nguyen K., Chau V.Q., Mauro A.G., Durrant D., Toldo S., Abbate A., Das A, & Salloum F.N. (2020). Hydrogen Sulfide Therapy Suppresses Cofilin-2 and Attenuates Ischemic Heart Failure in a Mouse Model of Myocardial Infarction. Journal of cardiovascular pharmacology and therapeutics, 25(5), 472-483.

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Reagents and Antibodies for Biomedical Research

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Disposable plastic was purchased from BD (BD Bioscience, Madrid, Spain). Biopsy punches were purchased from Kay Medical Europe Gmbh, Solingen, Germany. Vacuette® serum collection tubes were from Greiner Bio-One (Medline International Iberia S.L.U, Madrid, Spain). Sodium sulfide (Na2S), sodium salicylate, ascorbic acid, NaOH, paraformaldehyde, chloroform, 2-propanol and glycogen were purchased from Sigma-Aldrich (Sigma-Aldrich Química S.A, Madrid Spain). Antibodies for cystathionine γ lyase (CTH, ab54573) and cystathionine β synthase (CBS, Ab54883) were from Abcam® (Abcam® plc, Cambridge, UK). That of mercaptopyruvate sulfur transferase (3-MPST, sc-376168) was from Santa Cruz Biotechnology (Heidelberg, Germany). Reagents for tissue histology were from Merck, Panreac or Sigma-Aldrich except for DePex (Gurr®, VWR International Eurolab S.L. Barcelona, Spain). Reagents for immunohistochemistry were from Dako (Dako, Barcelona, Spain). Reagent for molecular biology were from Merck (absolute ethanol), Fermentas, Fisher

Burguera E.F., Vela-Anero Á., Gato-Calvo L., Vaamonde-García C., Meijide-Faílde R, & Blanco F.J. (2020). Hydrogen sulfide biosynthesis is impaired in the osteoarthritic joint. International journal of biometeorology, 64(6).

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Preparation and Analysis of Volatile Sulfur Compounds

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2.1 Reagents, standards and solvents DMS was obtained from Merck (Darmstadt, Germany). DMDS, EMS, DES, CS2, DEDS, MeSAc and EtSAc were supplied by Sigma-Aldrich (Steinheim, Germany). Sodium sulfide Na2S, sodium methanethiolate CH3SNa and sodium ethanethiolate CH3CH2SNa (also from Sigma-Aldrich) were dissolved in water at pH 9.6 for preparing H2S, MeSH and EtSH. These solutions were daily prepared and were kept in the anoxic chamber. Sodium metabisulfite (Na2S2O5) was purchased from Panreac (Barcelona, Spain). All the solutions and samples were prepared in the interior of a glove box P[BOX] (Jacomex) and they were stored out the glove box at -20°C, following recommendations of previous works [12, 14] .
Brine contained 350 g/L of NaCl (from Panreac, ARG quality, Barcelona, Spain) in Milli-Q water (Millipore, Billerica, MA, USA). Synthetic wine was a pure water solution containing 12% v/v ethanol (gradient grade for liquid chromatography, Merck), 5 g/L of tartaric acid and pH adjusted at 3.4 with an aqueous solution of NaOH (0.1 M).
Metal salts were CuSO4•5H2O, Fe(NH4)2(SO4)2•6H20 and ZnCl2 from Panreac, ARG quality. were used during the optimization of the analysis of Br-VSCs.

Ontañón I., Vela E., Hernández-Orte P, & Ferreira V. (2019). Gas chromatographic-sulfur chemiluminescent detector procedures for the simultaneous determination of free forms of volatile sulfur compounds including sulfur dioxide and for the determination of their metal-complexed forms. Journal of chromatography. A, 1596.

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