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Ix71 imaging system

Manufactured by Olympus
Sourced in United States

The IX71 imaging system is a versatile microscope platform designed for a wide range of imaging applications. It features a modular design and supports a variety of objectives, illumination sources, and imaging modes to meet the needs of researchers and scientists.

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5 protocols using ix71 imaging system

1

Wound Healing Assay for HUVECs

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Different pretreated HUVECs were seeded at a density of 2.0 × 105 cells/mL in a six‐well plate, and cells were allowed to adhere on the plate overnight. Then wounds were made using a 1 mL pipette tip, and the wells were washed with PBS to remove debris. Cells were cultured in fresh media containing 2% FBS. Photographs were taken using an Olympus IX71 Imaging System (Olympus Corporation) at 0 and 12 hours respectively. The experiments were carried out three times.
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2

Imaging Fluorescent Protein Transfection

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sf9 cells were transfected with different plasmid as described in the previous section and were imaged at 48 h post-transfection with an Olympus IX71 imaging system using an RFP light cube or bright field.
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3

Adipocyte Differentiation of C3H10T1/2 Cells

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The mesenchymal stem cell C3H10T1/2 was purchased from Procell Life Science & Technology Co. Ltd. (Wuhan, China). This cell shows fibroblast morphology and is functionally like mesenchymal stem cells (Pinney and Emerson, 1989 (link)). The C3H10T1/2 cells were maintained in minimum essential medium (MEM, HyClone, China) with 10% fetal bovine serum (FBS, Cell-max, Australia; F8318) in a 5% CO2 environment at 37°C. The medium containing 10% FBS, 125 nM indomethacin, 0.5 mM 3-isobutyl-1-methylxanthine, 1 μM rosiglitazone, 850 nM insulin, 1 nM triiodothyronine, and 1 μM dexamethasone was used for induction of adipocyte directional differentiation. Preadipocytes were cultured in the induced medium for 48 h to induce directional differentiation. These cells were then shifted to the medium with 1 μM rosiglitazone, 1 nM triiodothyronine, 850 nM insulin, and 10% FBS after 48 h and were harvested until the sixth day (Patrick et al., 2011 (link); Zhang et al., 2013 (link)). Subsequently, as observed using an Olympus IX71 imaging system (Olympus, Japan), at 200 X magnifications, the cells became round and exhibited lipid droplets. After cell differentiation, accumulated fats were stained using oil red (Dingguo Changsheng, China; A12989), and optical density was measured at 490 nm with a microplate reader.
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4

Immunocytochemistry Analysis of Rat Cardiomyocytes

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Immunocytochemistry was performed on in vitro cultured rat H9c2 cardiomyocytes. In brief, cardiomyocytes were washed in PBS and fixed with 4% paraformaldehyde solution for 40 min. Cells were permeabilized with 0.025% Triton-X-100 (v/v) solution in PBS for 40 min and blocked in 1% sterile BSA solution for 60 min at RT. After blocking, cells were washed and incubated in 1:100 diluted primary antibody anti-ANP (cat# GTX109255, GeneTex, United States) in PBS at 4°C for overnight. On the next day, cells were washed in PBS and incubated with 1:200 dilution of anti-rabbit Alexa®Fluor 488 conjugated secondary antibody (cat# A11008, Life Technologies, United States). Cardiomyocyte nuclei were counterstained with 1 μg/ml DAPI in PBS (cat #A1001, AppliChem, United States) and Alexa®Fluor 594 Phalloidin (cat# A12381, Life Technologies, United States) was used to counterstain the F-actin filaments. Fluorescence images were captured by Olympus IX71 Imaging Systems (Olympus, United States) and quantified with ImageJ software (NIH, United States).
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5

Quantifying F-Actin in H9c2 Cardiomyocytes

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Rat H9c2 cardiomyocytes were fixed with 4% paraformaldehyde for 40 min at RT, followed by permeabilization with 0.5% Triton-X-100 (v/v) in PBS for 40 min. After fixation, cells were incubated with Alexa Fluor®594 Phalloidin, 200 μM (cat# A12381, Life Technologies, United States) in PBS for 40 min and protected from direct light, and then washed with PBST. Images were captured by Olympus IX71 Imaging Systems (Olympus, United States) and the F-actin red intensity was quantified by ImageJ software (NIH, United States).
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