Anti rabbit hrp dab cell and tissue staining kit

Manufactured by R&D Systems

Sourced in Germany, United States

The Anti-Rabbit HRP-DAB Cell and Tissue Staining Kit is a laboratory tool used for the detection and visualization of target proteins in cell and tissue samples. The kit utilizes a horseradish peroxidase (HRP) conjugated secondary antibody and a 3,3'-Diaminobenzidine (DAB) chromogen to produce a brown stain at the location of the target protein. This kit is designed for use with rabbit primary antibodies.

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Lab products found in correlation

Smad2 3, by Santa Cruz Biotechnology (1 mentions) Anti goat hrp dab cell and tissue staining kit, by R&D Systems (1 mentions) Nel741001kt, by PerkinElmer (1 mentions) Bmpr 2, by Santa Cruz Biotechnology (1 mentions) Smad1 5 8, by Santa Cruz Biotechnology (1 mentions) P smad2 3, by Santa Cruz Biotechnology (1 mentions) Ab13847, by Abcam (1 mentions) Ab75183, by Abcam (1 mentions) F3777, by Merck Group (1 mentions) Alexa fluor 546 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Tcs sp5 confocal microscope, by Leica (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) Phospho erk1 2, by Cell Signaling Technology (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti‐rabbit HRP‐DAB Cell & Tissue Staining Kit HRP-DAB Cell and Tissue Staining Kit HRP‐DAB Cell & Tissue Staining Kit Anti-rabbit HRP-DAB staining kit

4 protocols using anti rabbit hrp dab cell and tissue staining kit

Immunohistochemical Analysis of Ovarian Development

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Ovaries derived from gilts at age of 30–400 days were fixed in 10% (w/v) paraformaldehyde with 0.02 MPBS (pH 7.2) at 4°C for about 2 h. Next, they were cut into vertical slices of about 0.5 cm thickness and fixed in fresh 10% (w/v) paraformaldehyde for a cumulative period of 24 h. These slices were mounted in paraffin, and serially cut into 5 μm-thick sections by Rotary Microtome (MICROM, Germany), and stained with HE. Ovarian HE sections were observed and photographed under a fluorescent microscope (Zeiss, Germany).
Immunohistochemistry detection was performed by using the anti-Rabbit HRP-DAB Cell and tissue staining kit (R&D, CTS005) and anti-Goat HRP-DAB Cell and tissue staining kit (R&D, CTS008). Immunohistofluorescence examination was performed by using TSA plus Fluorescein (PerkinElmer, NEL741001KT) and Cyanine3.5 (PerkinElmer, NEL763001KT) kit. Antibodies of BMP15 (Eterlife, EL806306-100), GDF9 (Eterlife, EL901942-100), FSHR (Eterlife, EL912710), luteinizing hormone receptor (LHR) (Eterlife, EL904141), Caspase3 (Abcam, ab13847), 3βHSD (Abcam, ab154385), p-Smad1/5 (CST, 9516), Ki67 (Abcam, ab15580) and LC3B (Arigo, ARG55799) were diluted 1:100 with PBS. Other antibodies including ALK6 (Santa Cruz, sc5679), BMPR2 (Santa Cruz, sc5683), Smad2/3 (Santa Cruz, sc8332), Smad1/5/8 (Santa Cruz, sc6031R), and p-Smad2/3 (Santa Cruz, sc11769) were diluted 1:50 in PBS.

Qin Y., Tang T., Li W., Liu Z., Yang X., Shi X., Sun G., Liu X., Wang M., Liang X., Cong P., Mo D., Liu X., Chen Y, & He Z. (2019). Bone Morphogenetic Protein 15 Knockdown Inhibits Porcine Ovarian Follicular Development and Ovulation. Frontiers in Cell and Developmental Biology, 7, 286.

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Assessing Pulmonary Fibrosis in Bleomycin-Treated Mice

Cited 2 times

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Mice were sacrificed at various time points after bleomycin treatment under anesthesia. The trachea was cannulated, and the left lungs were inflated with 0.5 mL of 10% neutral buffered formalin. Mouse lung tissue was fixed, embedded in paraffin, sectioned to 5 μm slices for Masson’s trichrome13 (link)49 (link) and BMPER staining. Anti-BMPER (ab75183, 1:200, Abcam) and anti-rabbit HRP-DAB cell and tissue staining kit were used according to the manufacturer’s instructions (R&D systems, Minneapolis, MN, USA). The semiquantitative Ashcroft score was used to score pulmonary fibrosis50 (link). In short, upon 200× magnification, each successive field was given a score ranging from 0 (normal) to 8 (total fibrous obliteration of the field). All scores from 5 sections were averaged41 (link).
Paraffin-embedded sections of normal and IPF lungs were used for immunofluorescence staining. Antibodies specific for BMPER (ab75183, 1:200, Abcam) and α-SMA (F3777, 1:250, Sigma, St. Louis, MO, USA) were used for staining. Alexa Fluor-546-conjugated secondary antibody was from Life Technologies. The nucleus were labeled with DAPI (Vector lab, Burlingame, CA, USA), photographed with a Leica TCS SP5 confocal microscope, and analyzed with Leica confocal software.

Huan C., Yang T., Liang J., Xie T., Cheng L., Liu N., Kurkciyan A., Monterrosa Mena J., Wang C., Dai H., Noble P.W, & Jiang D. (2015). Methylation-mediated BMPER expression in fibroblast activation in vitro and lung fibrosis in mice in vivo. Scientific Reports, 5, 14910.

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Paraffin Embedding and Immunohistochemistry

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Tissues were fixed in 10% NBF overnight and subsequently transferred into tissue cassettes and placed in 70% ethanol. The tissues were then paraffin embedded. Slides containing 4 μm sections were deparaffinized and hydrated by incubating them in two changes of xylene for five min each, followed by 2 changes of 100% ethanol for 3 min each, 70% ethanol for 2 min, 50% ethanol for 2 min, and distilled water for 5 min. Antigen retrieval was performed by incubating the slides in 10 mM Citric acid solution (pH 6.0) in an 80°C oven overnight. The slides were subsequently washed in PBS and permeabilized in 10% methanol containing 0.4% H₂O₂ for 30 min. After permeabilization, slides containing murine tissues were washed and stained with a rabbit anti human CD3 antibody (Abcam, Cambridge, MA; ab5690). All slides were subsequently stained using an anti-rabbit HRP-DAB cell and tissue staining kit according to the manufacturer's instructions (R&D systems, Minneapolis, MN).

Habiel D.M., Krepostman N., Lilly M., Cavassani K., Coelho A.L., Shibata T., Elenitoba-Johnson K, & Hogaboam C.M. (2016). Senescent stromal cell-induced divergence and therapeutic resistance in T cell acute lymphoblastic leukemia/lymphoma. Oncotarget, 7(50), 83514-83529.

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Immunohistochemical Analysis of Skin and Embryos

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Skin tissues and embryos were fixed overnight in 10% (w/v) paraformaldehyde with 0.02 MPBS (pH 7.2) at 4°C, processed, and mounted in paraffin, they were then serially cut into 5 μm-thick sections with a Rotary Microtome (MICROM). The histological sections were stained with hematoxylin and eosin (H&E) and then observed and photographed with a fluorescent microscope (Zeiss). For immunohistochemistry experiments, sections were treated with 3% H₂O₂ to quench endogenous peroxidase activity and then treated with 5% bovine serum albumin to block nonspecific protein binding sites. Sections were incubated with a primary antibody overnight at 4°C and then stained with an anti-Rabbit HRP-DAB Cell and tissue staining kit (R&D, CTS005). Detection was followed by TSA plus Fliorescein (Perkinelemer, NEL741001KT). All antibodies including KIT (Abcam Cat# ab47587, RRID : AB_868771), Phospho-KIT (Try719) (Cell Signaling Technology Cat# 3391, RRID : AB_2131153), Green Fluorescent Protein (Millipore Cat# AB3080P, RRID : AB_2630379), DCT (Abcam Cat# ab74073, RRID : AB_1524517), Erk1/2 (Cell Signaling Technology Cat# 4695, RRID : AB_390779), Akt (Cell Signaling Technology Cat# 9272, RRID : AB_329827) Phospho-Akt (Cell Signaling Technology Cat# 4060, RRID : AB_2315049), and Phospho-Erk1/2 (Cell Signaling Technology Cat# 8544, RRID : AB_11127856) were diluting 1:200 with PBS.

Sun G., Liang X., Qin K., Qin Y., Shi X., Cong P., Mo D., Liu X., Chen Y, & He Z. (2020). Functional Analysis of KIT Gene Structural Mutations Causing the Porcine Dominant White Phenotype Using Genome Edited Mouse Models. Frontiers in Genetics, 11, 138.

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