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Automaticwizard2gamma counter

Manufactured by PerkinElmer
Sourced in United States

The AutomaticWizard2 gamma counter is a laboratory instrument used for the detection and measurement of gamma radiation emitted by radioactive samples. It provides accurate and reliable quantification of gamma-emitting isotopes in various sample types.

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2 protocols using automaticwizard2gamma counter

1

Spectroscopic and Radioactivity Measurements

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All instruments were calibrated and
maintained according to the standard quality control practices and
procedures. UV–vis measurements were taken on a Shimadzu BioSpec-nano
microvolume UV–vis spectrophotometer (Shimadzu Scientific Instruments;
Kyoto, Japan). Radioactivity measurements were taken using a CRC-15R
dose calibrator (Capintec, Inc.; Ramsey, NJ, USA) and an automatic
Wizard2gamma counter (PerkinElmer; Waltham, MA, USA). Surface
plasmon resonance was performed using a Nicoya OpenSPR-XT instrument
(Nicoya Lifesciences; Kitchener, ON, Canada).
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2

Glucose Uptake Assay in NPC Cells

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Glucose uptake in NPC cells was determined using the 18F-fluorodeoxyglucose (18F-FDG) uptake assay as follows: The cells were cultured in 12-well plates (2 × 105/well) with 500 µL of DMEM containing 4 µCi/mL 18F-FDG at 37 °C for 1 hour. Subsequently, the pellets were washed with ice-cold phosphate buffered saline and lysed using 500 µL 0.1 M NaOH. The radioactivity of the total cell lysates was assayed using a PerkinElmer Automatic Wizard2 Gamma Counter.
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