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Lentiviral high titer packaging mix with plvsin series

Manufactured by Takara Bio
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The Lentiviral High Titer Packaging Mix with pLVSIN series is a set of plasmids designed for the production of high-titer lentiviral particles. The mix contains the necessary components for packaging lentiviral vectors, including the structural, enzymatic, and envelope proteins required for virus assembly and transduction.

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Plvsin cmv pur, by Takara Bio (1 mentions)

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Lentiviral High Titer Packaging Mix (25 citations) Lentiviral High-Titer Packaging Mix with pLVSIN (2 citations)

2 protocols using lentiviral high titer packaging mix with plvsin series

1

Stable Expression of Mouse MRP4

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To establish cells stably expressing mouse MRP4, full-length mouse Abcc4 cDNA was subcloned into lentiviral vectors under the control of the EF1α promoter. Lentivirus particles were prepared by the Lentiviral High Titer Packaging Mix with pLVSIN series (Clonetech, Palo Alto, CA) with Lenti-X 293 T cell lines. NIH3T3 cells were infected with MRP4-expressing lentivirus and maintained in a medium containing 5 µg/mL of puromycin.
Kato M., Tsurudome Y., Kanemitsu T., Yasukochi S., Kanado Y., Ogino T., Matsunaga N., Koyanagi S, & Ohdo S. (2020). Diurnal expression of MRP4 in bone marrow cells underlies the dosing-time dependent changes in the oxaliplatin-induced myelotoxicity. Scientific Reports, 10, 13484.
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2

Generation of mEGFP-expressing Lentivirus

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mEGFP-C1 plasmid was gifted from Michael Davidson (RRID:Addgene_54759). The sequence of mEGFP was subcloned into pLVSIN-CMV Pur (Takara). Lentivirus particles were prepared by the Lentiviral High Titer Packaging Mix with pLVSIN series (Clonetech, Palo Alto, California) using Lenti-X 293 T-cell lines. mEGFP-expressing lentivirus particles and 10 µg/mL of polybrene were added to the media of B16/BL6 cells, and then incubated for 24 hours. mEGFP-transduced cells were selected with 1 µg/mL of puromycin.
Tsuruta A., Shiiba Y., Matsunaga N., Fujimoto M., Yoshida Y., Koyanagi S, & Ohdo S. (2022). Diurnal Expression of PD-1 on Tumor-Associated Macrophages Underlies the Dosing Time-Dependent Antitumor Effects of the PD-1/PD-L1 Inhibitor BMS-1 in B16/BL6 Melanoma-Bearing Mice. Molecular Cancer Research, 20(6), 972-982.
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