Cells were plated in 24-well plates (106 cells/well) for 24 h and then 2.5 nM HApt-AuNS in growth media was added to each well. The cells were incubated with the nanoconstructs for 24 h at 37 °C in 5% CO2 environment. After incubation, excess nanoconstructs were removed from the wells, and the cells were washed twice with ice cold PBS (Gibco). The cells were then harvested and suspended in 100 µL PBS. The cells were counted using a hemocytometer before being digested for 4 h at 75 °C in acid mixture containing 200 µL of 30% HCl (Sigma-Aldrich) and 86 µL of 70% HNO3 (SigmaAldrich). After complete digestion of the AuNS, the solution was diluted with Millipore water to a final volume of 6 mL. The Au content was measured using ICP-MS.
Ice cold pbs
Manufactured by Thermo Fisher Scientific
Ice cold PBS is a pre-made, sterile phosphate-buffered saline solution that is chilled to a low temperature. It is designed to maintain a consistent pH and osmolarity for use in various laboratory applications.
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Lab products found in correlation
2 protocols using ice cold pbs
1
Cellular Uptake of Gold Nanoconstructs
2
Establishment of Liver Metastasis Pancreatic Tumor Model
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Establishment of the liver metastasis pancreatic tumor model as previously reported with slight modifications.20 In brief, 1 × 106 pancreatic cancer cells were suspended in 0.1 mL of ice‐cold PBS (Gibco). Laparotomy technique was performed as aforementioned and 0.2 mL of cell suspension was slowly injected into the spleen. After inoculation, the tumors were allowed to grow for 28 days and the mice were sacrificed by injecting 200 mg/kg pentobarbital intraperitoneally and the livers were dissected out. The surface metastases on the livers were counted after dissection of the livers into individual lobes.
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