Anti il 22

Manufactured by R&D Systems

Anti-IL-22 is a recombinant protein that binds to and neutralizes the activity of human Interleukin-22 (IL-22). IL-22 is a cytokine that plays a role in the regulation of immune responses and inflammation.

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Lab products found in correlation

Anti il 6, by R&D Systems (3 mentions) Lsrii system, by BD (2 mentions) Anti ccl20, by R&D Systems (2 mentions) Anti rorγt, by Thermo Fisher Scientific (2 mentions) Anti ifn γ, by R&D Systems (2 mentions) Anti il 17f, by R&D Systems (2 mentions) Anti il 17a, by Thermo Fisher Scientific (2 mentions) Anti t bet, by Thermo Fisher Scientific (2 mentions) Fortessa flow cytometer, by BD (2 mentions) Foxp3 staining buffer set, by Thermo Fisher Scientific (2 mentions) Facs caliber, by BD (2 mentions) Cytofix cytoperm plus kit, by BD (2 mentions) Anti il 17, by R&D Systems (1 mentions) Anti kim 1, by R&D Systems (1 mentions) Anti il 23, by R&D Systems (1 mentions) Anti cd3 and anti cd28, by BD (1 mentions) Anti il 8, by R&D Systems (1 mentions) Recombinant human ifn γ, by R&D Systems (1 mentions) Il 17, by R&D Systems (1 mentions) Il 22, by R&D Systems (1 mentions)

Spelling variants of this product

IL-22 (91 citations) Mouse anti–IL-22 antibody (4 citations) Anti-IL-22 PE (3 citations) PE-conjugated anti-IL-22 (2 citations) Anti-IL-22 antibody (2 citations)

6 protocols using anti il 22

Cytokine Profiling in Immune Cell Activation

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Recombinant human IFN-γ, IL-17, IL-22, IL-23, TNF-α, IL-6 and IL-8 were purchased from R&D Systems (Minneapolis, MN). Anti-human IFN-γ, anti-IL-17, anti- IL-22, anti-IL-23, anti-IL-6, anti-IL-8 and anti-KIM-1 antibodies were purchased from R&D Systems. Anti-CD3 and anti-CD28 were obtained from BD Biosciences (San Diego, CA), and 1,25(OH)₂D3 was obtained from Sigma (St. Louis, MO).

Chung B.H., Kim B.M., Doh K.C., Cho M.L., Kim K.W, & Yang C.W. (2017). Protective effect of 1α,25-dihydroxyvitamin D3 on effector CD4+ T cell induced injury in human renal proximal tubular epithelial cells. PLoS ONE, 12(2), e0172536.

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Immunohistochemical Analysis of Corneal Inflammation

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At the indicated time points, the corneas were enucleated, separated into epithelium and stroma with EDTA; the whole corneas as well as separated epithelial sheet were embedded in Tissue-Tek OCT compound, and frozen in liquid nitrogen. 6-μm thick sections were cut and mounted to poly-L-lysine-coated glass slides, fixed in 4% paraformaldehyde, blocked with PBS containing 2% BSA for 1 h at room temperature. Sections were then incubated with primary Abs: anti-NIMP-R14 (1:50; BD), anti-F4/80 (1:50; BD), anti-IL-22(1:200; R&D) anti-IL-36α (1:50; abcam), followed by the secondary Ab, FITC-conjugated IgG (1:100; Jackson ImmunoResearch Laboratories). Slides were mounted with Vectashield (Vector Laboratories, Burlingame, CA) mounting media containing DAPI. Controls were similarly treated with corresponding IgG from the same animal as the primary antibody.

Me R., Gao N., Zhang Y., Lee P.S., Wang J., Liu T., Standiford T.J., Mi Q.S, & Yu F.S. (2021). Interleukin-36α Enhances Host Defense against Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas. Journal of immunology (Baltimore, Md. : 1950), 207(11), 2868-2877.

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Isolation and Activation of CD4+ T Cells

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PBMCs were isolated as described before and erythrocytes were depleted. Next, CD4+ T cells were isolated with magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec). CD4+ T cells were incubated in RPMI 1640 Glutamax (Gibco) supplemented with 100 µM β-mercaptoethanol (Life Technologies) for 60 min 37°C and then stained extracellularly for CD4. Afterwards, 1×10⁶ cells were taken up in 500 µL prewarmed RPMI 1640 Glutamax (Gibco), supplemented with 100 U/mL penicillin/streptavidin (Gibco) and 10% FCS (Sigma) and incubated either with or without 20 ng/mL IL-23, 20 µg/mL anti-IL6 (BD Biosciences) and 2 µg/mL anti-IL22 (R&D) for 5 min at 37°C. Stimulation was stopped and cells were fixed by addition of cold 4% paraformaldehyde in phosphate buffered saline (PBS) and incubated for 10 min at room temperature. After a single wash with PBS, cells were permeabilised in 70% ice-cold methanol in PBS for 30 min on ice. The cells were then stained intracellularly for 30 min at room temperature with an antibody specific for phosphorylated STAT3 (pSTAT3) (BD Biosciences no. 557815) and analysed by flow cytometry.

Schmitt H., Billmeier U., Dieterich W., Rath T., Sonnewald S., Reid S., Hirschmann S., Hildner K., Waldner M.J., Mudter J., Hartmann A., Grützmann R., Neufert C., Münster T., Neurath M.F, & Atreya R. (2018). Expansion of IL-23 receptor bearing TNFR2+ T cells is associated with molecular resistance to anti-TNF therapy in Crohn’s disease. Gut, 68(5), 814-828.

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Real-Time Cytotoxicity Assay for T Cell-Mediated Killing

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In vitro real-time killing assays were performed by measuring electric impedance over time in an xCELLigence Real Time Cell Analysis Instrument (ACEA). 5x10³ B16OVA cells were seeded onto a 16-well plate (ACEA) and cultured for 4 h prior to the assay thereby allowing tumor cell adhesion. After the 4h-culture, 1x10³ Pmel-1 T cells that had received the indicated electroporation conditions with or without miRCURY miR-155 Inhibitor or a similarly synthetized irrelevant negative control (1.5 μM) (Qiagen) were added to the B16-OVA containing wells, in a 1:5 target-effector ratio. When indicated, for IFN-ɣ and IL-22 neutralization we added 2.5 μg/mL of anti-IFN-ɣ (XMG1.2, BioXcell) or anti-IL22 (R&D). Electric impedance was measured every 5 min for 96 h.

Olivera I., Bolaños E., Gonzalez-Gomariz J., Hervas-Stubbs S., Mariño K.V., Luri-Rey C., Etxeberria I., Cirella A., Egea J., Glez-Vaz J., Garasa S., Alvarez M., Eguren-Santamaria I., Guedan S., Sanmamed M.F., Berraondo P., Rabinovich G.A., Teijeira A, & Melero I. (2023). mRNAs encoding IL-12 and a decoy-resistant variant of IL-18 synergize to engineer T cells for efficacious intratumoral adoptive immunotherapy. Cell Reports Medicine, 4(3), 100978.

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Stimulation and Cytokine Profiling of T Cells

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T cells purified by cell sorting were stimulated with Leukocyte Activation Cocktail, with GolgiPlus^™ (BD Pharmingen) for 6 h at 37°C under 5% CO₂ before being stained with following antibodies alone or in combinations: anti-IL-17A (eBioscience), anti-IL-17F, anti-IL-22, anti-IL-6, anti-CCL20 and anti-IFNγ (R&D Systems) by using Cytofix/CytoPerm Plus kit (BD Pharmingen) or anti-RORγt, anti-FOXP3, anti-T-bet or anti-GATA3 using Foxp3 staining buffer set (eBioscience) according to the manufacturer’s instructions. All flow cytometry was done using FACSCaliber, LSR II System or Fortessa flow cytometers (BD Biosciences), and the data were subsequently analyzed using FlowJO software (TreeStar).

Singh S.P., Parween F., Edara N., Zhang H.H., Chen J., Otaizo-Carrasquero F., Cheng D., Oppenheim N.A., Ransier A., Zhu W., Shamsaddini A., Gardina P.J., Darko S.W., Singh T.P., Douek D.C., Myers T.G, & Farber J.M. (2023). Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticity. Journal of immunology (Baltimore, Md. : 1950), 210(11), 1700-1716.

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Intracellular Cytokine Analysis of T Cells

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T cells purified by cell sorting were stimulated with Leukocyte Activation Cocktail, with GolgiPlus ™ (BD Pharmingen) for 6 h at 37°C under 5% CO 2 before being stained with following antibodies alone or in combinations: anti-IL-17A (eBioscience), anti-IL-17F, anti-IL-22, anti-IL-6, anti-CCL20 and anti-IFNγ (R&D Systems) by using Cytofix/CytoPerm Plus kit (BD Pharmingen) or anti-RORγt, anti-FOXP3, anti-T-bet or anti-GATA3 using Foxp3 staining buffer set (eBioscience) according to the manufacturer's instructions. All flow cytometry was done using FACSCaliber, LSR II System or Fortessa flow cytometers (BD Biosciences), and the data were subsequently analyzed using FlowJO software (TreeStar).

Singh S.P., Parween F., Edara N., Zhang H.H., Chen J., Otaizo-Carrasquero F., Cheng D., Oppenheim N.A., Ransier A., Zhu W., Shamsaddini A., Gardina P.J., Darko S.W., Singh T.P., Douek D.C., Myers T.G, & Farber J.M. (2023). Human CCR6+ Th Cells Show Both an Extended Stable Gradient of Th17 Activity and Imprinted Plasticity. Journal of immunology (Baltimore, Md. : 1950), 210(11).

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