Human gastric cancer cell lines including AGS, MKN-45, and the immortalized human embryonic kidney epithelial cell line (HEK-293) were cultured in Dulbecco’s modified Eagle’s medium (GIBCO, Carlsbad, CA) supplemented with 10% fetal bovine serum (Invitrogen Life Technologies, Carlsbad, CA) and 1% penicillin/streptomycin (GIBCO). AGS and HEK-293 cells were purchased from American Type Culture Collection (ATCC, Manassas, VA). MKN-45 cells were obtained from the Riken Cell Bank (Tsukuba, Japan). All cell lines were ascertained to conform to the original in vitro morphological characteristics. Cycloheximide (CHX) was purchased from Sigma-Aldrich (St. Louis, MO). DARPP-32 (sc271111) and SRp20 (sc135) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Flag-tag antibody (F1804) was obtained from Sigma-Aldrich (St. Louis, MO). Horseradish peroxidase-conjugated mouse (7074P2) and rabbit (7062P2) secondary antibodies, ubiquitin (3933S), CD44 (3570S), and β-actin (4970) antibodies were purchased from Cell Signaling Technology (Danvers, MA).
Flag tag antibody f1804
Manufactured by Merck Group
The Flag-tag antibody (F1804) is a mouse monoclonal antibody that recognizes the Flag epitope tag. The Flag tag is a short peptide sequence (DYKDDDDK) that can be fused to a protein of interest, allowing for its detection and purification. The Flag-tag antibody is a useful tool for the identification and isolation of Flag-tagged proteins in various applications, such as immunoprecipitation, Western blotting, and immunoaffinity chromatography.
Automatically generated - may contain errors
Lab products found in correlation
Hek293, by American Type Culture Collection (2 mentions)
Mkn45, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
β actin 4970, by Cell Signaling Technology (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Cycloheximide (chx), by Merck Group (2 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions)
Mkn 45 cells, by RIKEN Cell Bank (2 mentions)
Darpp 32 sc271111, by Santa Cruz Biotechnology (2 mentions)
Srp20 sc135, by Santa Cruz Biotechnology (2 mentions)
Horseradish peroxidase conjugated mouse 7074p2 and rabbit 7062p2 secondary antibodies, by Cell Signaling Technology (2 mentions)
2 protocols using flag tag antibody f1804
1
Gastric Cancer Cell Line Cultures
2
Gastric Cancer Cell Line Cultures
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