A thin matrix layer was applied to the surface using an airbrush with a 0.2-mm nozzle (Procon Boy FWA Platinum, Mr. Hobby, Tokyo, Japan) [19 (link)]. The matrix was sprayed repeatedly for 15 sec at 15-sec intervals (for approximately 15 minutes) until 500 μL of 2,5-dihydroxybenzoic acid (2,5-DHB; Bruker Daltonics) solution (50 mg/mL in 70% methanol/0.1% trifluoroacetic acid) was used. During spraying, the distance between the nozzle and the tissue surface was maintained at 15 cm. Pulsed spraying resulted in the formation of a homogeneous matrix layer on the tissue surface.
2 5 dhb
Manufactured by Bruker
2,5-DHB is a matrix compound used in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It is designed to facilitate the ionization and detection of analytes in MALDI-MS experiments.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using 2 5 dhb
1
Homogeneous Matrix Layer Formation
2
MALDI-TOF Analysis of Compound Solutions
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MALDI-TOF
measurements: First, 1 μL of a DMSO solution of the compound
was spotted on a 384-MTP target plate (Bruker Daltonics, Bremen, Germany)
and air-dried. Subsequently, 1 μL of 2,5-dihydroxybenzoic acid
(2,5-DHB; Bruker Daltonics) matrix (20 mg/mL in ACN/water; 50:50 (v/v))
was applied on the plate, and the spots were left to dry prior to
MALDI-TOF analysis. An UltrafleXtreme MALDI-TOF (Bruker Daltonics),
equipped with Smartbeam-II laser, was used to measure the samples
in the reflectron positive ion mode. The MALDI-TOF was calibrated
using a peptide calibration standard prior to measurement.
measurements: First, 1 μL of a DMSO solution of the compound
was spotted on a 384-MTP target plate (Bruker Daltonics, Bremen, Germany)
and air-dried. Subsequently, 1 μL of 2,5-dihydroxybenzoic acid
(2,5-DHB; Bruker Daltonics) matrix (20 mg/mL in ACN/water; 50:50 (v/v))
was applied on the plate, and the spots were left to dry prior to
MALDI-TOF analysis. An UltrafleXtreme MALDI-TOF (Bruker Daltonics),
equipped with Smartbeam-II laser, was used to measure the samples
in the reflectron positive ion mode. The MALDI-TOF was calibrated
using a peptide calibration standard prior to measurement.
3
Protein and Peptide Calibration Standards
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Calibrant mixture (Protein Calibration Standard I and Peptide Calibration Standard II), a-cyano-4-hydroxycinnamic acid (a-cyano), and 2,5-DHB were purchased from Bruker Daltonics. The SA was purchased from Sigma. All other reagents were of spectrometry and chromatography grade.
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