The second part of the molecular examination was associated with determining the expression of adiponectin on the protein level by using the ELISA assay with the Adiponectin Human solid-phase sandwich Enzyme-Linked Immunosorbent Assay ELISA Kit (Life Technologies Corporation, Invitrogen, USA; catalog number: KHP0041) used. The ELISA assay consists of the following stages: binding the antigen; adding a detector antibody; then adding IgG HRP, TMB substrate solution; and stopping the solution according to protocol. Next, the plate was read at 450 nm and a standard curve generated to determine what the concentration of adiponectin was in the analyzed samples.
Adiponectin human solid phase sandwich enzyme linked immunosorbent assay elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Adiponectin Human solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) Kit is a laboratory tool used for the quantitative determination of Adiponectin levels in human serum, plasma, and other biological fluids. The kit utilizes the sandwich ELISA technique to detect and measure the target analyte.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using adiponectin human solid phase sandwich enzyme linked immunosorbent assay elisa kit
1
Quantifying Adiponectin Protein Levels
2
Evaluating Leptin and Adiponectin Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The third part of the molecular examination was determining the expression of leptin on the protein level through the use of the ELISA assay alongside the Adiponectin Human solid-phase sandwich Enzyme-Linked Immunosorbent Assay ELISA KIT (Life Technologies Corporation, Invitrogen, USA; Catalog Number; KAC2281). The ELISA assay consists of the following stages: binding to the antigen; adding the detector antibody; addition of IgG HRP and TMB substrate solutions; and, finally, stopping the solution according to protocol. Next, the plate was read at 450 nm and a standard curve was generated to determine the concentration of leptin in the analyzed samples.
To assess if the drug exerts its effect via the leptin path in the cell culture exposed to cisplatin and leptin siRNA compared to the control culture, the levels of JAK-2 (JAK2 ELISA Kit ab253224) and STAT3 (STAT3 ELISA KIT ab126427) were evaluated via the ELISA assay.
To assess if the drug exerts its effect via the leptin path in the cell culture exposed to cisplatin and leptin siRNA compared to the control culture, the levels of JAK-2 (JAK2 ELISA Kit ab253224) and STAT3 (STAT3 ELISA KIT ab126427) were evaluated via the ELISA assay.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!