Cba caolahsd

Animal experiments were approved in advance by, and conducted under licence of, the ethics committee of the government authority of the federal state of Northrhine Westphalia (NRW) (Landesamt für Naturschutz, Umweltschutz und Verbraucherschutz, NRW), and were conducted in accordance with the European Communities Council Directive of September 22nd, 2010 (2010/63/EEC). All methods were carried out in accordance with the relevant guidelines and regulations. Sample size, outcome measures, statistical measures and experimental procedures are consistent with the ARRIVE guidelines 2.0. The design of the study did not necessitate ‘blinding’ or randomisation. Male CBA/J (Charles River, Sulzfeld, Germany) and CBA/CaOlaHsd mice (Envigo Laboratories, Venray, Netherlands) were housed in a temperature- and humidity-controlled containers (Scantainer, Scanbur Technology A/S, Karlslunde, Denmark) with a constant 12-h light–dark cycle and ad libitum food and water access. The CBA/J (PDE6brd1/PDE6brd1) mouse possesses a hereditary mutation of the PDE6B gene (retinal degeneration1, rd1)^{42 (link),43 (link)} that results in retinal destruction by 4 weeks of age^{7 (link)}. The CBA/CaOlaHsd mouse strain has no reported deficits in its sensory modalities^{44 (link)–46 (link)} and exhibits superior spatial memory compared to the CBA/J strain^{7 (link),8} .

The present study was carried out in accordance with the European Communities Council Directive of 22 September 2010 (2010/63/EEC) for care of laboratory animals. Male CBA/J (Charles River) and CBA/CaOlaHsd mice (Harlan (Envigo) Laboratories) were group housed in a temperature- and humidity-controled vivarium with a constant 12-h light–dark cycle (lights on from 6 AM to 6 PM) with ad libitum food and water access. The CBA/J (PDE6brd1/PDE6brd1) mouse expresses a hereditary mutation of the PDE6B gene (rd1) (Pittler and Baehr 1991 (link); Cohen et al. 2001 (link)). The CBA/CaOlaHsd mouse (in contrast, for example, to the C57BL/6 mouse) has a circadian cycle-sensitive visual system (Jin and Ribelayga 2016 (link)), with no reported deficits in other sensory modalities (Brooks et al. 2004 (link); Walton et al. 2008 (link); Heckman et al. 2017 (link)) and equivalent cognitive abilities to a variety of lab mouse strains (Brooks et al. 2005 (link)).

Fifteen male mice of both CBA/Ca OlaHsd (Envigo, UK) and C57BL/6J OlaHsd (Comparative Medicine Unit, Trinity College Dublin) were purchased; all mice were 8 weeks old at the time of the experiment. Five mice of each strain received oral gavage (Instech, FTP-20-38-50, USA) with either 1000 eggs of A. suum, 1000 eggs of A. lumbricoides or 100 μL 0.05 m of H₂SO₄. The mice were culled at day 7 p.i. and the livers and spleens removed for flow cytometric analysis of immune cells. Additionally, the lungs were removed for larval counts using the modified Baermann method (Lewis et al., 2006 (link)).