Mcc950
MCC950 is a small-molecule inhibitor that selectively targets the NLRP3 inflammasome. It is a laboratory research tool used to study the role of the NLRP3 inflammasome in various cellular and biochemical processes.
Lab products found in correlation
11 protocols using mcc950
Inflammasome Activation and Inhibition Assays
LG Epithelial Cell Isolation and Treatments
We treated the primary LG epithelial cells with MCC950 (10 µM/mL; TargetMol) for 24 hours in order to inhibit the NLRP3 inflammasome. For antioxidation experiments, the primary LG epithelial cells were treated with an N-acetyl-L-cysteine (NAC) antioxidant (1 mM; Sigma, St. Louis, MO, USA) for 24 hours. Thereafter, the treated primary LG epithelial cells of passage 2 were collected for Oil Red O (ORO) staining, JC-1 staining, TUNEL staining, and Western blotting.
NLRP3 Inflammasome Activation Assay
CRISPR/Cas9-Mediated AQP5 Knockout Mice
Mice were intraperitoneally injected with MCC950 (100 µL, 20 mg/kg, TargetMol, Boston, MA, USA) once a week for 8 times in total. Then the samples were taken 1 week after the last injection of MCC950 for testing.
Inflammasome Activation Assay
Induction of Accelerated Systemic Lupus Nephritis in NZB/WF1 Mice
Immune Activation Reagents Protocol
NLRP3 Inflammasome Activation Assay
Investigation of Inflammatory Pathways
Anti-ASC (1:1000, sc-22514-R) was purchased from Santa Cruz Biotechnology (Dallas, USA). Anti-GAPDH (1:2000, 60004-1-1g) was purchased from Proteintech (Chicago, USA). Color Prestained Protein marker (20AB01) was purchased from GenStar (Beijing, China).
Investigation of Inflammatory Pathways
Anti-ASC (1:1000, sc-22514-R) was purchased from Santa Cruz Biotechnology (Dallas, USA). Anti-GAPDH (1:2000, 60004-1-1g) was purchased from Proteintech (Chicago, USA). Color Prestained Protein marker (20AB01) was purchased from GenStar (Beijing, China).
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