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Nupage mes sds running buffer 20x

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NuPAGE™ MES SDS Running Buffer (20X) is a pre-made, concentrated solution used for electrophoresis of protein samples. It is a 20X concentrated buffer designed for use with Thermo Fisher Scientific's NuPAGE™ electrophoresis systems.

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Lab products found in correlation

Nupage lds sample buffer 4x, by Thermo Fisher Scientific (2 mentions) Precision plus protein westernc standard, by Bio-Rad (1 mentions) Xcell surelock mini cell electrophoresis system, by Thermo Fisher Scientific (1 mentions) Histrap ff crude column, by GE Healthcare (1 mentions) Nupage sample reducing agent 10x, by Thermo Fisher Scientific (1 mentions) Seeblue plus2 pre stained protein standard, by Thermo Fisher Scientific (1 mentions) Nupage novex 4 12 bis tris protein gel, by Thermo Fisher Scientific (1 mentions) Snakeskin dialysis tubing, by Thermo Fisher Scientific (1 mentions) Linbro plates, by Hampton Research (1 mentions) Western lightning plus ecl, by PerkinElmer (1 mentions) Microscope slide, by Thermo Fisher Scientific (1 mentions) Oct compound, by Thermo Fisher Scientific (1 mentions) Ponceau s solution, by Merck Group (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Protease and phosphatase inhibitors tablets, by Thermo Fisher Scientific (1 mentions) Prolong diamond antifade mountant with or without dapi, by Thermo Fisher Scientific (1 mentions) Laemmli sample buffer, by Bio-Rad (1 mentions) Seeblue plus2 pre stained standard, by Thermo Fisher Scientific (1 mentions) Rapid cbb kanto, by Kanto Chemical (1 mentions)

Spelling variants of this product

NuPAGE MES SDS running buffer (157 citations) MES running buffer (120 citations) MES SDS running buffer (114 citations) NuPAGE MES Running Buffer (19 citations) MES-SDS buffer (17 citations) NuPAGE MES SDS buffer (8 citations) NuPAGE running buffer (6 citations) NuPage MES Buffer (5 citations) NuPAGE MES SDS Running (2 citations) NuPAGE® MES-SDS (2 citations)

4 protocols using nupage mes sds running buffer 20x

1

SDS-PAGE Protein Separation and Analysis

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The samples were mixed in the ratio of 3:1 with NuPAGE™ LDS sample buffer (4X) (Life Technologies™, Carlsbad, CA, United States). All solutions were then heated at 70°C for 10 min before being added to a precast NuPAGE™ Bis–Tris Gel 10% (Invitrogen, Carlsbad, CA, United States) in the XCell SureLock™ Mini-Cell electrophoresis system (Life Technologies™, Carlsbad, CA, United States). The system was prepared with 1X NuPAGE™ MES SDS Running Buffer (20X) (Life Technologies™, Carlsbad, CA, United States) and 10–250 kDa Precision Plus Protein™ WesternC™ standards (Bio-Rad, Hercules, CA, United States). Electrophoresis was then performed at 100 V for 10 min and subsequently at 140 V for 65 min, and finally, the gel was rinsed with MilliQ water before blotting.
Clegg L.A., Sloth J.K., Bæk R, & Jørgensen M.M. (2022). Photometric method for dual targeting of surface and surface-associated proteins on extracellular vesicles in the multiparametric test. Frontiers in Molecular Biosciences, 9, 917487.
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2

Purification and Analysis of NSP10 Variants

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10 kDa centrifuge filters were purchased from Sigma-Aldrich. Five ml HisTrap FF crude columns were bought from GE Healthcare. NuPAGE MES SDS Running Buffer (20 X), SeeBlue Plus2 Pre-stained Protein Standard, SimpleBlueTM SafeStain, NuPAGE Sample Reducing Agent (10 X), NuPAGE LDS Sample Buffer (4 X) and NuPAGENovex 4–12% Bis-Tris Protein Gels (1.0 mm 10 well) were obtained from Life Technologies. Quick StartTM Bradford 1 x Dye Reagent was from Bio-Raid. SnakeSkin Dialysis Tubing was purchased from Thermo Scientific. Linbro plates were bought from Hampton Research. NT. 115 premium capillaries were obtained from Nanotemper. NSP10 expression clones for mutants T12I, T102I, and A104V optimized for E. coli expression was purchased from Genscript.
Wang H., Rizvi S.R., Dong D., Lou J., Wang Q., Sopipong W., Su Y., Najar F., Agarwal P.K., Kozielski F, & Haider S. (2023). Emerging variants of SARS-CoV-2 NSP10 highlight strong functional conservation of its binding to two non-structural proteins, NSP14 and NSP16. eLife, 12, RP87884.
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3

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis

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NuPAGE® MOPS SDS Running Buffer (20X), (#NP0001–02), NuPAGE® MES SDS Running Buffer (20X), (#NP0002–02), NuPAGE™ 4–12% Bis-Tris Protein Gels, 1.0 mmX15well, (#NP0323BOX), NuPAGE™ 4–12% Bis-Tris Protein Gels, 1.0 mmX26well, (#WG1403BOX) and PierceTM BCA Protein Assay Kit (#23225) and ProLong Diamond antifade mountant with or without DAPI (#P36962 or #P36961) were from Thermo Scientific. Western Lightning Plus ECL (#NEL105001EA) was from PerkinElmer. Immobilon®-FL Transfer membrane 0.45 um, Polyvinylidene Difluoride (PVDF) membrane (#ISEQ00010) was from Merck Millipore. HyBlot CL films (#E3012) were from Denville Scientific, Inc. Non-fat dry milk (#M0841) was from Lab Scientific. Protease and Phosphatase inhibitors tablets (#88669) were from Thermo Scientific. Dynabeads Protein G was from Novex (Life Technologies, #10004D). Ponceau S Solution (#P7170) was from Sigma-Aldrich. OCT compound (#23–730-571) and microscope slides (# 12–550-15) were from Fisher Scientific. Liquid blocker pap pen (#71310) was from Electron Microscopy Sciences (EMS).
Lachance V., Wang Q., Sweet E., Choi I., Cai C.Z., Zhuang X.X., Zhang Y., Jiang J.L., Blitzer R.D., Bozdagi-Gunal O., Zhang B., Lu J.H, & Yue Z. (2019). Autophagy protein NRBF2 has reduced expression in Alzheimer’s brains and modulates memory and amyloid-beta homeostasis in mice. Molecular Neurodegeneration, 14, 43.
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4

SDS-PAGE Protein Separation and Visualization

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The sample solution was mixed 1:1 with Laemmli Sample buffer (Bio-Rad Laboratories, Inc., California, USA) containing 5 % 2-ME. The sample was boiled at 100 °C for 5 min and cooled in the flowing water. The sample and a molecular weight marker (SeeBlue Plus2 Prestained Standard, Thermo Fisher Scientific, CA, USA) were applied at 10 μL/lane to the NuPAGE 10 % Bis-Tris Gel 1.0 mm*15well (Thermo Fisher Scientific, CA, USA) in a running buffer (NuPAGE MES SDS Running Buffer (20X), Thermo Fisher Scientific, CA, USA). The electrophoresis was performed at a constant-voltage (200 voltage) for 30 min using an XCell SureLock Mini-Cell apparatus (Thermo Fisher Scientific, CA, USA) under reducing conditions. The gels were stained with Rapid CBB KANTO (Kanto Chemical Co., Inc., Tokyo, Japan).
Tomiki M., Sakai M., Tanaka D., Hosoya M., Uchida K., Shibata H., Morita M., Ito R., Iwasaki Y, & Akiyama H. (2024). A reliable enzyme-linked immunosorbent assay for the determining of sesame proteins in raw food ingredients and in processed foods. Food Chemistry: X, 21, 101231.
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