Accu chek active meter

The control rate, which was defined as HbA1c level lower than 7%, and the change in HbA1c values were the primary endpoint [18 ]. HbA1c was measured using affinity HPLC method (Automatic HA-8160 Analyzer HA-8160, Arkray Factory, Inc., Shiga, Japan). Fingertip blood was collected and FPG was measured using a blood glucose analyzer (ACCU-CHEK Active Meter, Roche Diagnostics, Indianapolis, USA). The 2hPG level was measured after taking a standardized meal (Olympus AU640 Analyzer, Olympus Optical Co., Ltd., Shizuoka, Japan). β-cell function was evaluated from venous blood to determine plasma insulin and C-peptide level, using the homeostatic model assessment (HOMA) to quantify HOMA insulin resistance (HOMA-IR) and β-cell function (HOMA-β) [19 (link)].
Blood pressure (BP) was collected at each visit. Total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) were measured by enzymatic methods (Olympus AU640 Analyzer, Olympus Optical Co., Ltd., Shizuoka, Japan).
All the changes in test results and symptoms of each outpatient should be recorded in detail, and we collected the records of the first visit and 6, 12, 18, 24, 36, 48, 60 months from the first visit.

ITT was conducted 4 weeks after virus injection and GTT 5 weeks after virus injection. Mice were habituated to daily intraperitoneal injections of isotonic saline 3 days before each tolerance test. All procedures were started at 10:00 and performed with reference to general procedures [32 (link), 33 (link)]. For ITT, 6 h-fasted mice were injected with 1.0 U insulin/kg (Sigma, Cat#I9278) and blood was collected from the tail vein at the designated times and used to measure glucose with a glucose monitor (Roche, Accu-Chek Active meter). GTT was performed using 16 h-fasted mice by an i.p. injection of 1.5 g/kg glucose (Sigma, Cat#G8270) and blood glucose was assessed as in ITT.

BALB/c athymic nude mice (male, 5 weeks old) were purchased from Shanghai Experimental Animal Center (Chinese Academy of Sciences, China). Animal care and experiments were carried out in accordance with guidelines of the Xi'an Jiaotong University. BALB/c athymic nude mice were grouped into euglycemia, hyperglycemia, euglycemia + PEG-CAT, and hyperglycemia + PEG-CAT groups (n = 6), of which hyperglycemia mice received an intraperitoneal injection of STZ dissolved in sodium citrate buffer (pH 4.5) at a dose of 175 mg/kg body weight. Blood glucose levels were determined with an ACCU-CHEK Active meter (Hoffmann-La Roche, Basel, Switzerland).