For patients included in the present study, fasting blood samples at admission were drawn and stored at −80°C for the following lipid profiles analysis.
Reagents: Water, acetonitrile and isopropyl alcohol were obtained from Fisher Scientific (Pittsburgh, PA, USA). Formic acid (>98%) and ammonium acetate (>99%) were obtained from Fluka (Buchs, Switzerland). Free fatty acids standards were obtained from Nu‐Chek‐Prep (Elysian, MN, USA).
Samples were thawed at 4°C. Formic acid and ammonium acetate plus water or acetonitrile and isopropyl alcohol were used as the mobile phase. C19:0 was used as the internal standard. Liquid chromatography–tandem mass spectrometry analysis was carried out with Eksigent LC100 and AB SCIEX Triple TOF 5600 (AB SCIEX, Framingham, MA, USA). Eksigent LC100 was equipped with XBridge Peptide BEH C18 Column (Waters, Milford, MA, USA). PeakView1.2 (AB SCIEX) was used for qualitative analysis. MultiQuant2.1 (AB SCIEX) was used for quantitative analysis.
Reagents: Water, acetonitrile and isopropyl alcohol were obtained from Fisher Scientific (Pittsburgh, PA, USA). Formic acid (>98%) and ammonium acetate (>99%) were obtained from Fluka (Buchs, Switzerland). Free fatty acids standards were obtained from Nu‐Chek‐Prep (Elysian, MN, USA).
Samples were thawed at 4°C. Formic acid and ammonium acetate plus water or acetonitrile and isopropyl alcohol were used as the mobile phase. C19:0 was used as the internal standard. Liquid chromatography–tandem mass spectrometry analysis was carried out with Eksigent LC100 and AB SCIEX Triple TOF 5600 (AB SCIEX, Framingham, MA, USA). Eksigent LC100 was equipped with XBridge Peptide BEH C18 Column (Waters, Milford, MA, USA). PeakView1.2 (AB SCIEX) was used for qualitative analysis. MultiQuant2.1 (AB SCIEX) was used for quantitative analysis.