Fiber photometry recordings were performed using head-fixed mice that were free to run on a circular treadmill. Fiber optic cables (1 m long; 400 μm core; 0.48 NA; Doric Lenses) were coupled to implanted optic fibers with zirconia sleeves (Precision Fiber Products). Black heat shrink material was placed around the fiber coupling to prevent external sources of light (e.g. from the visual stimulus) from interfering with recordings. Excitation and emission light was passed through a GFP fluorescence minicube (FMC3_E(460–490)_F(500–550); Doric Lenses). Excitation light (~ 100 μW) was provided by a 465 nm LED (Plexon LED and driver) which was modulated at either 217 Hz or 319 Hz using TTL output from two lock-in amplifiers (SR830; Stanford Instruments). Emission light was collected by a femtowatt photoreceiver (Newport 2151), demodulated using a lock-in amplifier (SR830; Stanford Instruments) and digitized at 1 kHz sample rate (PCIe-6321; National Instruments). Data acquisition was controlled using a custom script in MATLAB (MathWorks).
Fmc3 e 460 490 f
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The FMC3_E(460–490)_F is a laboratory equipment product. It has a core function of measuring within a specified wavelength range, but a more detailed description cannot be provided while maintaining an unbiased and factual approach.
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2 protocols using fmc3 e 460 490 f
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Fiber Photometry Recordings in Head-Fixed Mice
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Fiber Photometry Recordings in Head-Fixed Mice
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Fiber photometry recordings were performed using head-fixed mice that were free to run on a circular treadmill. Fiber optic cables (1 m long; 400 μm core; 0.48 NA; Doric Lenses) were coupled to implanted optic fibers with zirconia sleeves (Precision Fiber Products). Black heat shrink material was placed around the fiber coupling to prevent external sources of light (e.g. from the visual stimulus) from interfering with recordings. Excitation and emission light was passed through a GFP fluorescence minicube (FMC3_E(460–490)_F(500–550); Doric Lenses). Excitation light (~ 100 μW) was provided by a 465 nm LED (Plexon LED and driver) which was modulated at either 217 Hz or 319 Hz using TTL output from two lock-in amplifiers (SR830; Stanford Instruments). Emission light was collected by a femtowatt photoreceiver (Newport 2151), demodulated using a lock-in amplifier (SR830; Stanford Instruments) and digitized at 1 kHz sample rate (PCIe-6321; National Instruments). Data acquisition was controlled using a custom script in MATLAB (MathWorks).
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