Goat anti human iga

Manufactured by Southern Biotech

Sourced in United States

Goat anti-human IgA is a laboratory reagent used to detect and quantify human immunoglobulin A (IgA) in biological samples. It is produced by immunizing goats with purified human IgA and isolating the specific antibodies. This product can be used in various immunoassay techniques, such as ELISA, Western blot, and immunoprecipitation, to measure IgA levels in research and diagnostic applications.

Automatically generated - may contain errors

Lab products found in correlation

Goat anti mouse igm, by Southern Biotech (1 mentions) Syto bc, by Thermo Fisher Scientific (1 mentions) Biotinylated goat anti mouse iga, by Southern Biotech (1 mentions) Streptavidin apc, by BioLegend (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Protease inhibitor, by Merck Group (1 mentions) Ir800 label, by LI COR (1 mentions) Rabbit anti human igg, by Thermo Fisher Scientific (1 mentions) Zeba spin desalting column, by Thermo Fisher Scientific (1 mentions) 4 laser fortessa, by BD (1 mentions) Hrp conjugated goat anti mouse igg, by Southern Biotech (1 mentions) 96 well plate, by Corning (1 mentions)

Close spelling variants of this product

Alkaline phosphatase labeled goat anti human IgG or IgA antibodies Horseradish peroxidase–conjugated goat anti‐human IgA, Goat anti-human IgA antibody Goat F(ab)2 anti-human IgA Goat Anti-Human IgA-HRP FITC)-labeled goat F(ab)2 anti-human IgA Goat anti-human IgA horseradishperoxidase Goat-anti-human IgA-PE Anti-human IgA

4 protocols using goat anti human iga

Quantifying Intestinal Antibody Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

Homogenized intestinal contents were resuspended at 0.1 mg/μL in PBS with protease inhibitors (Sigma), homogenized, and centrifuged at 400g to remove large debris. Supernatant was filtered through a sterile 70 μm strainer and centrifuged at 8000g to pellet bacteria. This supernatant was collected and assayed for free Ig by ELISA. The bacterial pellet was resuspended in PBS 0.25% BSA with SYTO BC (Life Technologies) and 5% goat serum and then stained with biotinylated goat anti-mouse IgA, goat anti-human IgA, or goat anti-mouse IgM (Southern Biotech). After washing, bacteria were stained with streptavidin-APC (BioLegend). Bacteria were washed and resuspended in PBS 0.25% BSA with DAPI (Life Technologies) prior to flow cytometry using a low FSC and SSC threshold to allow bacterial detection. See also Supplemental Experimental Procedures.

Bunker J.J., Flynn T.M., Koval J.C., Shaw D.G., Meisel M., McDonald B.D., Ishizuka I.E., Dent A.L., Wilson P.C., Jabri B., Antonopoulos D.A, & Bendelac A. (2015). Innate and adaptive humoral responses coat distinct commensal bacteria with immunoglobulin A. Immunity, 43(3), 541-553.

+ Open protocol

+ Expand

Fluorescent Antibody Labeling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

IR800 label (100 μg) (Licor, Westburg, Netherlands) was dissolved in 25 μl distilled water. Subsequently, IR800 label was conjugated to secondary antibodies by mixing 100 μl goat-anti-human IgA (Southern Biotech, USA, Alabama) or rabbit-anti-human IgG (Thermo Fisher, Netherlands) with 1 or 2 μl IR800 label, respectively. Unbound IR label was removed from the samples using ZEBA spin desalting column (Thermo Fisher, Netherlands).

Raeven R.H., van der Maas L., Pennings J.L., Fuursted K., Jørgensen C.S., van Riet E., Metz B., Kersten G.F, & Dalby T. (2019). Antibody Specificity Following a Recent Bordetella pertussis Infection in Adolescence Is Correlated With the Pertussis Vaccine Received in Childhood. Frontiers in Immunology, 10, 1364.

+ Open protocol

+ Expand

Antibody Binding to Unpermeabilized Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

We measured antibody binding to whole (un-permeabilized) un-infected HEp-2 cells by flow cytometry. Briefly, we collected HEp-2 cells and washed 3× with DPBS-BSA before counting. ~1 million cells/condition were stained with a final concentration of 100 μg/mL, 10 μg/mL, or 1 μg/mL antibody diluted in DPBS-BSA for 20 min at 4C. Cells were then washed 3× with DPBS-BSA and stained with either goat anti-human IgG labeled with PE (Southern Biotech) or goat anti-human IgA (Southern Biotech) labeled with PE diluted 1:1,000 in DPBS-BSA for 20 min at 4C. Cells were washed a final time and fluorescence acquired on a 4-Laser Fortessa (BD Biosciences). FCS files were analyzed, and figures generated using CytoBank. Data shown is representative of at least 2 separate experiments with different antibody preparations. The following reagent was obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH: Anti-Human Immunodeficiency Virus (HIV)-1 gp41 Monoclonal Antibody (4E10), ARP-10091, contributed by DAIDS/NIAID.

Pilewski K.A., Wall S., Richardson S.I., Manamela N.P., Clark K., Hermanus T., Binshtein E., Venkat R., Sautto G.A., Kramer K.J., Shiakolas A.R., Setliff I., Salas J., Mapengo R.E., Suryadevara N., Brannon J.R., Beebout C.J., Parks R., Raju N., Frumento N., Walker L.M., Fechter E.F., Qin J.S., Murji A.A., Janowska K., Thakur B., Lindenberger J., May A.J., Huang X., Sammour S., Acharya P., Carnahan R.H., Ross T.M., Haynes B.F., Hadjifrangiskou M., Crowe JE J.r., Bailey J.R., Kalams S., Morris L, & Georgiev I.S. (2023). Functional HIV-1/HCV cross-reactive antibodies isolated from a chronically co-infected donor. Cell reports, 42(2), 112044.

+ Open protocol

+ Expand

Evaluating Antibody Responses via ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols

SIF treated samples were evaluated for immunoreactivity using antigen-specific ELISA. For IgG and mIgA ELISA, Corning polystyrene 96-well plates (#9018) were coated overnight at 4 °C with antigen (LT, gp120, or CfaE) at 100 ng/well followed by blocking with ELISA buffer (1X Blocker BSA [ThermoFisher #37525], 0.05% Tween-20 in PBS). SIF samples were diluted in ELISA buffer to a final concentration of 1 μg/mL based upon the initial concentration of antibody used in the assay and titrated 2-fold. Following a thirty minute incubation, plates were washed 3x with PBST (PBS + 0.05% Tween-20) and incubated with horseradish peroxidase conjugated Goat-anti-human IgG (Southern Biotech #2040-03) or Goat-anti-human IgA (Southern Biotech #2050-05) in ELISA buffer for thirty minutes. After a second wash step, plates were developed using a TMB 2-Component Microwell Peroxidase Substrate Kit (SeraCare #5120-0047) and background corrected absorbance (A₄₅₀) was determined using a Biotech Epoch plate reader with Gen5 software. In the case of dIgA and SIgA, ELISA was carried out essentially as described above with the exception of using a monoclonal mouse anti-human J-chain antibody followed by HRP-conjugated Goat-anti-mouse IgG (Southern Biotech #1030-05) for detection.

Wallace A.L., Schneider M.I., Toomey J.R., Schneider R.M., Klempner M.S., Wang Y, & Cavacini L.A. (2020). IgA as a potential candidate for enteric monoclonal antibody therapeutics with improved gastrointestinal stability. Vaccine, 38(47), 7490-7497.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!