Younger (8 months) and older (19 months) male and female C57/B6 mice were purchased from the National Institute on Aging aged rodent colony. Male and Female rTg4510 mice and littermate controls (2 months of age) were purchased from the Jackson Laboratory. Mice were housed four per cage on a 12‐h light/dark cycle at 22–24°C at the laboratory animal facility at Colorado State University. All animals were given free access to water and food (Teklad Global Irradiated 18% protein rodent diet) and acclimatized to the facility before being randomly assigned to experimental groups. Body weights, food consumption, and water intake were measured every week, and all animals were routinely monitored by veterinary staff. All animal procedures were approved by Colorado State University, IACUC protocol #1441.
Rtg4510 mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
The RTg4510 mice are a laboratory animal model used in research. They are genetically modified mice with specific genetic characteristics. The core function of the RTg4510 mice is to serve as a research tool for scientific investigations, without further interpretation or extrapolation on their intended use.
Automatically generated - may contain errors
4 protocols using rtg4510 mice
1
Aged Mouse Model Characterization
2
Aged Transgenic Mouse Brain Fixation
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Four rTg4510 mice (Jackson Laboratories, Bar Harbor, ME, USA) and four nontransgenic littermates were euthanized at 9 months by Somnasol overdose followed by transcardial perfusion with 0.9% saline. The left hemisphere was obtained from each mouse and fixed overnight in 4% paraformaldehyde followed by cryo-protection in sucrose gradients of 10%, 20% and 30%. Brains were frozen on a freezing stage and horizontally sectioned on a sliding microtome at 25 µm then stored in a PBS solution containing 0.02% NaN3 at 4 °C. All animal studies were approved by the University of South Florida Institutional Animal Care and Use Committee and carried out in accordance with the National Institutes of Health (NIH) guidelines for the care and use of laboratory animals (Aproval Code M1309, approved July 15, 2015).
3
Long-Term 3TC Treatment Mitigates Tauopathy-Induced Cognitive Deficits
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This was a retrospective study of samples from our previous report on 3TC and brain aging/pathology, in which we reported group differences in cognitive function in tauopathy mice with 3TC treatment (Wahl et al., 2023) (link). rTg4510 mice and appropriate littermate controls (2 months of age) were purchased from the Jackson Laboratory (strain number 024854). 3TC (Lamivudine; National Drug Code: 33342-002-07) was purchased from Health Warehouse (Florence, KY, USA) and added to drinking water at a concentration of 2 mg/mL, a concentration that has been used to increase cognitive function in mice in previous studies (Simon et al., 2019; (link)Wahl et al., 2023) (link) with no reported adverse effects. Mice were maintained on a 12 h/12 h reverse light cycle, and experimental cohorts were treated with 3TC for 6weeks, while controls were provided with the same water but without the drug. Fresh water was routinely provided to all cages every week, regardless of treatment. All animals were routinely health checked by the veterinary staff at Colorado State University. All protocols were approved by Colorado State University Laboratory Animal Resources (RRID: SCR_022157; IACUC protocol #1441).
4
Transgenic Mouse Models for Tauopathies
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All protocols involving mice were approved by the Institutional Animal Care and Use Committee of Baylor College of Medicine. rTg4510 mice were obtained from Jackson Labs and maintained by crossing the transactivator line CaMKIIα (129S6 background) with the tau responder line (FVB background; Ramsden et al., 2005 (link); SantaCruz et al., 2005 (link)). Wild-type littermates were used as controls. PS19 mice were obtained from Jackson Labs (Yoshiyama et al., 2007 (link)). Heterozygotes were bred to B6C3F1/J wild-type mice to maintain the line. The conditional tcfeb-flox mice were a generous gift from A. Ballabio (Telethon Institutes of Genetics and Medicine, Naples, Italy; Settembre et al., 2012 (link)) and crossed to nestin-Cre mice from Jackson Labs. Control mice were littermate tcfeb-flox homozygotes without the cre.
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