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Cd8 buv805 clone sk1

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CD8-BUV805 (clone SK1) is a fluorochrome-conjugated antibody that binds to the CD8 antigen. CD8 is a glycoprotein expressed on the surface of certain T cells and NK cells. The BUV805 fluorochrome is used for flow cytometry analysis.

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Lab products found in correlation

Tim3 bv510 clone f38 2e2, by Thermo Fisher Scientific (2 mentions) Cd45ro pe cy7 clone uchl1, by BD (2 mentions) Cd45ra fitc or bv711 clone hi100, by BD (2 mentions) Ccr7 bv421 clone 150503, by BD (2 mentions) Cd122 bv510 clone mik β3, by BD (2 mentions) Cd4 buv395 clone sk3, by BD (2 mentions) Cd22 fc and her2 fc recombinant proteins, by R&D Systems (2 mentions) Dylight488 and or 650 antibody labeling kits, by Thermo Fisher Scientific (2 mentions) Cd3 pacblue clone hit3a, by Thermo Fisher Scientific (2 mentions) Cd45 percp cy5.5 clone hi30, by BD (2 mentions) Cd62l bv605 clone dreg 56, by BD (2 mentions) Vα 7.2 pe clone 3c10, by BioLegend (1 mentions) Live dead fixable blue viability dye, by Thermo Fisher Scientific (1 mentions) Cd25 bv421 clone m a251, by BD (1 mentions) Foxp3 alexa fluor 488 clone 259d c7, by BD (1 mentions) Cd4 apc r700 clone rpa t4, by BD (1 mentions) Ctla 4 pe cf594 bni3, by BD (1 mentions)

Close spelling variants of this product

CD8 (clone SK1, CD8-BUV805 CD8 (SK1, BUV805

3 protocols using cd8 buv805 clone sk1

1

Comprehensive Immunophenotyping of T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-CD19 CAR idiotype antibody was kindly provided by Bipulendu Jena and Laurence Cooper48 . The 1A7 anti-14G2a idiotype antibody was obtained from NCI-Frederick. CD22 and Her2 CARs were detected using human CD22-Fc and Her2-Fc recombinant proteins (R&D). The idiotype antibodies and Fc-fusion proteins were conjugated in house with Dylight488 and/or 650 antibody labeling kits (Thermo Fisher). T cell surface phenotype was assessed using the following antibodies:
From BioLegend: CD4-APC-Cy7 (clone OKT4), CD8-PerCp-Cy5.5 (clone SK1), TIM3-BV510 (clone F38–2E2), CD39-FITC or APC-Cy7 (clone A1), CD95-PE (clone DX2), CD3-PacBlue (clone HIT3a),
From eBioscience: PD1-PE-Cy7 (clone eBio J105), LAG3-PE (clone 3DS223H), CD45RO-PE-Cy7 (clone UCHL1), CD45-PerCp-Cy5.5 (clone HI30),
From BD: CD45RA-FITC or BV711 (clone HI100), CCR7-BV421 (clone 150503), CD122-BV510 (clone Mik-β3), CD62L-BV605 (clone DREG-56), CD4-BUV395 (clone SK3), CD8-BUV805 (clone SK1).
Lynn R.C., Weber E.W., Sotillo E., Gennert D., Xu P., Good Z., Anbunathan H., Lattin J., Jones R., Tieu V., Nagaraja S., Granja J., DeBourcy C., Majzner R., Satpathy A.T., Quake S.R., Monje M., Chang H, & Mackall C.L. (2019). c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature, 576(7786), 293-300.
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2

Comprehensive Immunophenotyping of T-cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
LIVE/DEAD Fixable Blue Viability Dye (Invitrogen), CD25 BV421 (clone M-A251, BD), CD3 BUV661 (clone UCHT1, BD Biosciences), CD8 BUV805 (clone SK1, BD Biosciences), CD4 APCR700 (clone RPA-T4, BD Biosciences), CD161 BV605 (clone DX12, BD), Vα7.2 PE (clone 3C10, BioLegend), IL-7Rα BV786 (clone HIL-7R-M21, BD Biosciences), Foxp3 Alexa Fluor 488 (clone 259D/C7, BD Biosciences), Helios PerCP-eFluor 710 (clone 22F6, Invitrogen), CTLA-4 PECF594 (BNI3, BD Biosciences).
Berkson J.D., Slichter C.K., DeBerg H., Delaney M.A., Woodward-Davis A.S., Maurice N.J., Lwo Y., Ko A., Hsu J., Chiu Y.W., Linsley P.S., Dixon D, & Prlic M. (2020). Inflammatory cytokines induce sustained CTLA-4 cell surface expression on human MAIT cells. ImmunoHorizons, 4(1), 14-22.
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3

Comprehensive Immunophenotyping of T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-CD19 CAR idiotype antibody was kindly provided by Bipulendu Jena and Laurence Cooper48 . The 1A7 anti-14G2a idiotype antibody was obtained from NCI-Frederick. CD22 and Her2 CARs were detected using human CD22-Fc and Her2-Fc recombinant proteins (R&D). The idiotype antibodies and Fc-fusion proteins were conjugated in house with Dylight488 and/or 650 antibody labeling kits (Thermo Fisher). T cell surface phenotype was assessed using the following antibodies:
From BioLegend: CD4-APC-Cy7 (clone OKT4), CD8-PerCp-Cy5.5 (clone SK1), TIM3-BV510 (clone F38–2E2), CD39-FITC or APC-Cy7 (clone A1), CD95-PE (clone DX2), CD3-PacBlue (clone HIT3a),
From eBioscience: PD1-PE-Cy7 (clone eBio J105), LAG3-PE (clone 3DS223H), CD45RO-PE-Cy7 (clone UCHL1), CD45-PerCp-Cy5.5 (clone HI30),
From BD: CD45RA-FITC or BV711 (clone HI100), CCR7-BV421 (clone 150503), CD122-BV510 (clone Mik-β3), CD62L-BV605 (clone DREG-56), CD4-BUV395 (clone SK3), CD8-BUV805 (clone SK1).
Lynn R.C., Weber E.W., Sotillo E., Gennert D., Xu P., Good Z., Anbunathan H., Lattin J., Jones R., Tieu V., Nagaraja S., Granja J., DeBourcy C., Majzner R., Satpathy A.T., Quake S.R., Monje M., Chang H, & Mackall C.L. (2019). c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature, 576(7786), 293-300.
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