B6 mrl faslpr mice

Sh3bp2 P416R gain-of-function mutation KI heterozygous (Sh3bp2^KI/+) mice were generated by introducing a Pro-to-Arg mutation into exon 9 of the murine Sh3bp2 gene, as reported previously [15 (link),31 (link)]. B6.MRL-Fas^lpr mice (C57BL/6J background; referred to as Fas^lpr mice) were obtained from the Jackson Laboratory (Bar Harbor, ME, USA). All wild-type (WT) and mutant mice were maintained in the animal facility of Kawasaki Medical School (Okayama, Japan). All mice were housed in groups (2–5 mice/cage) and maintained at 22 °C under a 12 h:12 h light/dark cycle with free access to water and standard laboratory food (MF diet, Oriental Yeast Co., Tokyo, Japan). All animal experiments were approved by the Safety Committee for Recombinant DNA Experiments (Nos. 14-33 and 18-23) and the Institutional Animal Care and Use Committee of Kawasaki Medical School (Nos. 17-042 and 17-131). All experimental procedures were conducted in accordance with the institutional and National Institutes of Health guidelines for the humane use of animals.