According to the manufacturer's recommendation, the oxygen saturation level during the emphysema phase was measured via a paw sensor on Days 0 (prior to elastase instillation), 2, 9, and 16 using MouseSTAT Jr (Kent Scientific, Torrington, CT, USA) on conscious mice. Five 10-s readings were averaged and presented as the results.
Mousestat jr
Manufactured by Kent Scientific
Sourced in United States
The MouseSTAT Jr. is a compact and portable physiological monitoring system designed for preclinical research applications. It provides real-time monitoring of vital signs, including heart rate, respiration rate, and body temperature, in small animal models such as mice. The device is designed for ease of use and reliable data collection to support various research studies.
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Lab products found in correlation
9 protocols using mousestat jr
1
Measuring Oxygen Saturation in Emphysema
2
Intravital Imaging of Mouse Ileum Microcirculation
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Mice were anesthetized by intraperitoneally injection of ketamine (0.1 g/kg BW) and 0.01 g xylazine (0.01 g/kg BW) (injection solution: 10 μl/g BW). Mice were placed on a preheated plate at 37 °C, and the depth of anesthesia was monitored by checking withdrawal reflexes and using the MouseSTAT® Jr (Kent Scientific) heart monitor. A small piece of the ileum (~1 cm) was taken, longitudinally open, feces were flushed out, and the tissue was fixed on a preheated metal plate using agarose gel and tissue glue. After preparation of the ileum, mice were injected intravenously with Qtracker™ 655 vascular labels (Invitrogen) to provide real-time vascular contrast. Image acquisition was done using a 2-photon microscope (Olympus FVMPE-RS). Afterwards, mice were euthanized. Quantification was made using Imaris Stitcher and Imaris software (Bitplane) to manually construct surface modules from the fluorescence intensity. The surface area was measure within a surface bounding box area.
3
Intranasal Inoculation of Mice with H1N1 IAV
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H1N1 IAV was purchased from ATCC, aliquoted, and stored at –80°C until use. Viral titers were confirmed using viral plaque assays. Mice were anesthetized using isoflurane and inoculated intranasally with 600 PFU IAV in 40 μL of inoculum. After infection, mice were monitored daily for clinical score and oxygen saturation (MouseSTAT Jr., Kent Scientific). Clinical scores were determined as described previously. Briefly, a score of 0 indicated a normal, healthy mouse, 1 indicated slightly ruffled fur, 2 indicated ruffled fur, 3 indicated ruffled fur and inactive behavior, 4 indicated hunched back and moribund, and 5 indicated dead. The researcher who reported the clinical scores was not blinded to the experimental groups.
4
Cranial Window Implantation in Mice
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C57Bl6 mice were anesthetized with isoflurane [3% induction, 1 to 1.5% maintenance, in oxygen (1 liter/min)] during surgery and imaging sessions. After removal of the scalp, the skull was removed to fit the placement of the crystal skull (51 (link)). The glass then was sealed with dental acrylic, and the animal was recovered for 3 weeks before the imaging session. During surgery and imaging, heart rate and oxygen saturation were noninvasively monitored (Mouse Stat Jr., Kent Scientific), and all noted measurements were within the expected physiological range.
5
Cardiovascular Measurements in Mice
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Before the mice were sacrificed, blood pressure, oximetry, and heart rate were recorded. Blood pressure was measured using the CODA monitor (Kent Scientific). The tails of mice were inserted into the tail cuff and blood pressure was taken. Heart rate and oximetry were measured using the MouseSTAT® Jr. (Kent Scientific). Readings were taken from the paw of the mice.
6
Multielectrode Neurophysiology in Anesthetized Rats
7
Non-Invasive Vagus Nerve Stimulation in Mice
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pVNS was performed as pervious described [24 (link)]. Mice were under anesthesia with 6% induction sevoflurane (Covertrus, Dublin, OH, USA) then were placed on a heatpad (39 °C) with nose-cone delivered 3–5% sevoflurane to maintain general anesthesia. Pulse-oximetry (MouseSTAT Jr.; Kent Scientific Corporation, Torrington, CT) was applied to monitor the real time heart rate. VNS approach under ultrasound guidance and non-invasive pulse oximetry monitoring ascertain the effects of vagus stimulation on sympathetic activity (0.2–1mA to achieve 90% bradycardia threshold, 20 Hz, 0.3 ms/pulse biphasic).
8
Stereotaxic Electrode Implantation for Electrophysiology
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Stereotaxic surgeries were performed to implant electrodes in animals for the electrophysiology recording. The rat was anesthetized with an injection of ketamine (40 mg/kg) and xylazine (12 mg/kg), and remained anesthetized throughout the surgery by 0.75% isoflurane delivered through an endotracheal catheter intubated (PhysioSuite, Kent Scientific Corporaition). Antibiotics baytril (2.5 mg/kg) and long-acting analgesic drug buprenorphine were intramuscularly administered. During surgery, the temperature was monitored and maintained by a warming pad (PhysioSuite, Kent Scientific Corporaition). Heart rate and SpO2 were monitored with a pulse oximetry (MouseSTAT Jr, Kent Scientific Corporation). An MR-compatible electrode (MRCM16LP, NeuroNexus Inc) was unilaterally implanted into the ACC (coordinates: anterior/posterior +1.5, medial/lateral +0.5, dorsal/ventral −2.8). The reference wire and grounding wire from the electrode were both connected to a silver wire placed in the cerebellum. After surgery, the animal was returned to the home cage and allowed to recover for at least 1 week.
9
Multielectrode Neurophysiology in Anesthetized Rats
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Adult male rats (Long Evans, Charles River, Wilmington, MA, 350 – 400 g) were initially anesthetized by ketamine (40 mg/kg) and xylazine (12 mg/kg). Oxygen without or with isoflurane (0.5 – 1.5%) was ventilated through a nose cone during the whole surgery. Heart rate, body temperature, and SpO2 were monitored (MouseSTAT Jr., Kent Scientific Corporation). A warming pad (PhysioSuite, Kent Scientific Corporation) was used to keep the body temperature at 35 – 37 °C. Three different kinds of electrodes were used in this study: homemade 4-channel tungsten electrode (0.1 – 0.2 MΩ), 4-channel Q-trode (~0.4 MΩ, NeuroNexus, Ann Arbor, MI), and 16-channel chronic electrode (~0.4 MΩ, NeuroNexus, Ann Arbor, MI). The electrode was lowered to the right mV1 (−6.6 to bregma, +3.2 to midline). The uppermost recording site of each electrode was positioned right below the pial surface (~100 μm).
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