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Dig rna label mix

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The DIG RNA Label Mix is a laboratory reagent used for the synthesis of digoxigenin (DIG)-labeled RNA probes. It contains the necessary components, including nucleotides and enzymes, to facilitate the in vitro transcription and labeling of RNA probes with DIG. DIG-labeled RNA probes are commonly used in applications such as Northern blotting, in situ hybridization, and ribonuclease protection assays to detect and analyze specific RNA targets.

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Lab products found in correlation

T3 rna polymerase, by Promega (2 mentions) Alkaline phosphatase, by Roche (1 mentions)

2 protocols using dig rna label mix

1

In Situ Hybridization of Myelin Genes

Cited 1 time
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In situ hybridization was performed on fresh frozen sagittal brain sections (18 μm), using digoxigenin (DIG) -labeled antisense probes for murine Plp1/Dm-20, Mbp and Enpp6 as described previously30 (link),53 (link),54 (link). The probe was synthesized by T3 RNA polymerase (Promega, Cat#P208C) and labeled with DIG RNA label mix (Roche, Cat#11277073910). An anti-DIG antibody conjugated with alkaline phosphatase (Roche, Cat#11093274910) was used to probe sections and followed by stained with 5-bromo-4-cloro-3-indolyl phosphate (BCIP) (Solarbio, Cat#B8090) /nitro blue tetrazolium (NBT) (Solarbio, Cat#N8140) chromogenic substrates.
Zhang K., Chen S., Yang Q., Guo S., Chen Q., Liu Z., Li L., Jiang M., Li H., Hu J., Pan X., Deng W., Xiao N., Wang B., Wang Z.X., Zhang L, & Mo W. (2022). The Oligodendrocyte Transcription Factor 2 OLIG2 regulates transcriptional repression during myelinogenesis in rodents. Nature Communications, 13, 1423.
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2

In situ Hybridization of Galc in Mouse Brain

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Galc in situ hybridization was performed with a slight modification of the previous method84 (link). Cryosections of brain were incubated with digoxigenin (DIG)-labeled antisense riboprobes for murine Galc. The probe was synthesized using T3 RNA polymerase (Promega) and labeled with DIG RNA label mix (Roche). An anti-DIG antibody conjugated with alkaline phosphatase (Roche) was used to probe sections, which were stained with 5-bromo-4-cloro-3-indlyl phosphate/nitro blue tetrazolium (Roche) chromogenic substrates.
Weinstock N.I., Kreher C., Favret J., Nguyen D., Bongarzone E.R., Wrabetz L., Laura Feltri M, & Shin D. (2020). Brainstem development requires galactosylceramidase and is critical for pathogenesis in a model of Krabbe disease. Nature Communications, 11, 5356.
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