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Tetracaine hydrochloride ophthalmic solution

Manufactured by Bausch & Lomb
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Tetracaine hydrochloride ophthalmic solution is a local anesthetic medication used to numb the eye. It temporarily reduces pain and discomfort in the eye.

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Lab products found in correlation

Tropicamide ophthalmic solution, by Bausch & Lomb (1 mentions) Balanced salt solution, by Alcon (1 mentions) Phenylephrine hydrochloride ophthalmic solution, by Bioteck (1 mentions) Gonak hypromellose ophthalmic demulcent solution, by Akorn (1 mentions) Speedvac, by Labconco (1 mentions)

2 protocols using tetracaine hydrochloride ophthalmic solution

1

Anesthetic and Ophthalmic Solutions for Animal Research

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All reagents were in sterile packages, and medication and drops were filtered to ensure sterility. They included the following:

Ketamine hydrochloride solution 100 mg/mL (Dechra Veterinary Products, Overland Park, KS)

Xylazine 20 mg/mL (AnaSed, Shenandoah, IA)

Phenylephrine hydrochloride ophthalmic solution 2.5% (Paragon BioTeck, Portland, OR)

Tropicamide ophthalmic solution 1% (Bausch & Lomb, Rochester, NY)

GONAK hypromellose ophthalmic demulcent solution 2.5% (Akorn Pharmaceuticals, Lake Forest, IL)

Tetracaine hydrochloride ophthalmic solution 0.5% (Bausch & Lomb)

Balanced salt solution 15 mL (Alcon, Fort Worth, TX)

Clidox-S solution (Pharmacal Research Laboratories, Waterbury, CT)

Movahedan A., Barba H., Spedale M., Deng N., Arvans D., Nadeem U., Leone V., Chang E.B., Theriault B, & Skondra D. (2021). Gnotobiotic Operations and Assembly for Development of Germ-Free Animal Model of Laser-Induced Choroidal Neovascularization. Translational Vision Science & Technology, 10(9), 14.
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2

Phospholipid Extraction from Aqueous Humor

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DBA/2J mice were anesthetized by an intraperitoneal injection of a cocktail of Ketamine and Xylazine. A drop of tetracaine hydrochloride ophthalmic solution (0.5%, Bausch & Lomb, USA) was used to anesthetize the ocular surface prior to AH procurement. Normotensive and hypertensive AH was obtained from the anterior chamber of DBA/2J mice via paracentesis using a syringe (catalog no. 7635-01 Hamilton, Reno, Nevada) with a small 33 gauge removable needle (701RN, catalog no. 7803-05 Hamilton). A total of ~3 μl of AH was collected per mice and used for phospholipid extraction using modified Bligh and Dyer extraction method[13 (link),14 (link)]. Phospholipids extracted in the lower organic phase were dried using a Speed-Vac (Model 7810014; Labconco, Kansas City, MO), and then flushed with argon gas for oxidation prevention. Proteins procured from the upper aqueous phase were quantified using a suitable modification of Bradford’s method.
Wang H., Edwards G.T., Garzon C., Piqueras C, & Bhattacharya S.K. (2015). Aqueous Humor Phospholipids of DBA/2J and DBA/2J-Gpnmb+ /SjJ mice. Biochimie, 113, 59-68.
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