The animals were divided into 2 groups. One group was treated with FLX pellets (Innovative Research of America, Sarasota, FL) for 4 weeks at a dose of 3 mg/kg/day, whereas the other group was treated with control pellets (Innovative Research of America) for the same period. This dosage was chosen based on results of a previous study showing behavioral alterations in marmosets receiving the same dose [14 (link)]. Animals were anesthetized with an intramuscular injection of ketamine hydrochloride (50–60 mg/kg) and medetomidine hydrochloride (0.1–0.15 mg/kg), followed by atipamezole hydrochloride injection of 0.5–0.75 mg/kg. FLX or control pellets were implanted subcutaneously on their backs [15 (link)].
Control pellets
Manufactured by Innovative Research
Control pellets are laboratory equipment used as a reference or baseline in research experiments. They serve as a point of comparison to help evaluate the effects of treatments or interventions on experimental subjects. The core function of control pellets is to provide a standardized, untreated sample that can be used to assess the impact of the variables being tested.
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Lab products found in correlation
2 protocols using control pellets
1
Controlled Fluoxetine Delivery in Primates
2
Ovariectomy-Induced Breast Cancer Xenograft Model
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Four-week-old female BALB/c (nu/nu) mice were obtained from Sun Yat-sen University. All animals were bred and housed at the Animal Experiment Center of Zhongshan School of Medicine, Sun Yat-sen University. The care and use of all animals followed the guidelines for laboratory animals, and the protocol was approved by Sun Yat-sen University Animal Policy and Welfare Committee. The mice were anaesthetized and underwent a bilateral ovariectomy (OVX) through a 1-cm dorsal incision. Meanwhile, E2 pellets (0.72 mg, 60-day release, Innovative Research) and control pellets (Innovative Research) were implanted subcutaneously. After surgery, the mice were allowed to recover for 1 week, and tumours were established by injecting MDA-MB-468 cells (5 × 106 in 100 ml of Matrigel mixture) into the mammary fat pads of the mice. Callipers were used to measure the size of tumours every 5 days, and tumour volumes were calculated using the formula TV = length × [width] 2 × 0.5. Forty-five days later, the mice were sacrificed, and the tumour specimens were harvested for further study.
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