Milli q gradient machine

All reagents and solvents used within the
synthesis and purification were purchased from commercial sources
(Sigma-Aldrich, Fischer-Scientific, Acros-Organics or Fluorochem Ltd.)
and used without prior purification unless otherwise stated. Dry solvents
(EtOH, tetrahydrofuran (THF), and MeOH) were transferred to Schlenk
flasks and kept over predried 4 Å molecular sieves prior to use.
3′-Sialyllactose sodium salt and 6′-sialyllactose sodium
salt were purchased from Biosynth. SBA, Jacalin, WGA, SNA, and EBL
were purchased from Vector Laboratories. d-galactose and
sheep blood in Alsever’s were purchased from Merck. Ultrahigh-quality
water with a resistance of 18.2 MΩ·cm (at 25 °C) was
obtained from a Millipore Milli-Q gradient machine fitted with a 0.22
μM filter.

All chemicals were used as supplied unless otherwise stated. Methanol, hexane, hydrochloric acid, dichloromethane, toluene, acetone, tetrahydrofuran and diethyl ether were purchased from Fisher Scientific at laboratory reagent grade unless otherwise stated. Deuterochloroform (99.9 atom% D), 4,4′-azobis(4-cyanovaleric acid) (>97.0%), dodecane thiol (≥98.0%), potassium phosphate tribasic (reagent grade, ≥98.0%), carbon disulfide (≥99.9%), 2-bromo-2-methylpropionic acid (98.0%), N-isopropylacrylamide (97.0%), mesitylene (analytical standard) and magnesium sulfate (≥99.5%) were all purchased from Sigma-Aldrich. Ultrahigh quality water with a resistance of 18.2 MΩ cm (at 25 °C) was obtained from a Millipore Milli-Q gradient machine fitted with a 0.22 μm filter. Gold nanoparticle solutions for 15 nm (0.242 mmol L^–1) and 40 nm (0.296 mmol L^–1) were purchased from BBI Solutions. Pre-formulated, powdered, phosphate buffered saline was purchased from Sigma-Aldrich, and the desired solution made by addition of ultrahigh quality water to give [NaCl] = 0.137 M, [KCl] = 0.0027 M, [Na₂HPO₄] = 0.01 M, [KH₂PO₄] = 0.0018 M and pH = 7.4.