NMR spectra (1H, 13C and COSY) were collected on Agilent 600 MHz DD2. Mass spectrometry was performed using a Bruker Agilent 1290-micrOTOF Q II. Fluorescence spectra were measured on a PerkinElmer LS-55 machine, with an excitation wavelength of 366 nm.
Dd2 600 mhz
Manufactured by Agilent Technologies
Sourced in United States
The DD2 600 MHz is a high-performance nuclear magnetic resonance (NMR) spectrometer designed by Agilent Technologies. It operates at a frequency of 600 MHz and is capable of analyzing a wide range of samples for various scientific and research applications.
Automatically generated - may contain errors
8 protocols using dd2 600 mhz
1
Characterization of Novel Compounds
2
NMR Spectroscopy of Reaction Mixtures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1H-NMR spectra were obtained from ~5 mg of EC and the corresponding reaction mixture suspended in 0.8 mL acetone-d 6. The spectra were recorded on an Agilent 600 MHz DD 2 with a 5 mm One NMR probe (Agilent Technologies, Santa Clara, CA, USA). Integration of the spectra was performed with Mnova NMR software (version 6.1.0, Mestrelab Research S. L., Santiago de Compostela, Spain).
3
Lignin Characterization by 2D-HSQC-NMR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The extracted BW-LCC fractions were structurally characterized on an NMR spectrometer (Bruker spectrometer TYPE-AVIII 400 MHz, Bruker Co., Billerica, Massachusetts, USA). The LCC was dissolved in 0.75 mL of DMSO-d6 by sonication for 30 min. Subsequently, 2D HSQC NMR experiments were performed using Burker’s ’hsqcetgpsisp 2.2′ adiabatic pulse program with 4000 Hz and 20,000 Hz spectral widths in the 1H- and 13C-dimensions, respectively. Using a cryogenically cooled 3 mM HCN automated triple resonance probe and an Agilent DD2 600 MHz instrument, NMR spectra were recorded at 25 °C. The number of collected complex points was 2048 for the 1H-dimension, with a recycle delay of 1.5 s. 1H and 13C dimensions were processed using matched Gaussian apodization and squared cosine-bell apodization, respectively. The data matrix was Fourier transformed after 1024 points were filled in the 13C dimension. The central solvent peak was used as an internal reference.
The relative quantification of the lignin fraction based on 2D-HSQC-NMR was performed in Mestrenova11 using the integral (Int) for 2D HSQC spectra, where S = 0.5IntS2,6 and G = IntG2. Relative quantification of the linkage within BW-LCC was performed using the normalization method, the following equation was used to calculate the relative quantification of lignin linkage:
The relative quantification of the lignin fraction based on 2D-HSQC-NMR was performed in Mestrenova11 using the integral (Int) for 2D HSQC spectra, where S = 0.5IntS2,6 and G = IntG2. Relative quantification of the linkage within BW-LCC was performed using the normalization method, the following equation was used to calculate the relative quantification of lignin linkage:
4
NMR Spectroscopy at 14.1 T and 20.0 T
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NMR measurements at 14.1 T were performed using Agilent DD2 600 MHz spectrometer equipped with a 3.2 mm narrow-bore triple resonance T3 style probe and 3.2 mm zirconium oxide rotors. The resonance frequency of the 14N overtone signal at this field is expected to be 86.7448 MHz. All 14.1 T spectra were referenced to this frequency.
NMR measurements at 20.0 T were performed using 850 MHz Bruker spectrometer equipped with a 3.2 mm wide-bore triple resonance probe and 3.2 mm zirconium oxide rotors. At this field the 14N overtone transitions are expected to be located at 122.8331 MHz. All 20.0 T spectra were referenced to this frequency.
NMR measurements at 20.0 T were performed using 850 MHz Bruker spectrometer equipped with a 3.2 mm wide-bore triple resonance probe and 3.2 mm zirconium oxide rotors. At this field the 14N overtone transitions are expected to be located at 122.8331 MHz. All 20.0 T spectra were referenced to this frequency.
5
Characterization of Methacrylated Gelatin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To verify the successful methacrylation
of gelatin in GelMA and the intactness of the methacryloyl group on
GelMA@GFs, the chemical structures of GelMA, GelMA@VEGF165, and GelMA@bFGF were characterized using Agilent DD2 600 MHz NMR.
The1H NMR spectra were acquired by dissolving GelMA, GelMA@VEGF165, and GelMA@bFGF at a concentration of 8 mg/mL in deuterium
oxide (D2O). All measurements were done at 40 °C,
and the D2O peak was identified at 4.671 ppm.
of gelatin in GelMA and the intactness of the methacryloyl group on
GelMA@GFs, the chemical structures of GelMA, GelMA@VEGF165, and GelMA@bFGF were characterized using Agilent DD2 600 MHz NMR.
The1H NMR spectra were acquired by dissolving GelMA, GelMA@VEGF165, and GelMA@bFGF at a concentration of 8 mg/mL in deuterium
oxide (D2O). All measurements were done at 40 °C,
and the D2O peak was identified at 4.671 ppm.
6
Purification and Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents and chemicals were obtained commercially (Bide Pharmatech Ltd, China). The solvents were dried and used according to standard procedures, and all chemical reactions were monitored by thin layer chromatography (TLC) and observed under 254 and 365 nm UV irradiation on TLC plates or by potassium permanganate staining. Compounds were purified by column chromatography on silica gel (200 – 300 mesh).1H, 13CNRM resonance spectra were measured on a Bruker AM-300 (1H, 400 MHz; 13C, 100 MHz) or Agilent DD2 600 MHz (1H, 600 MHz; 13C, 151 MHz) with TMS as an internal standard. The solvent was deuterated chloroform (CDCl3 or DMSO-d6), and the chemical shifts were in ppm. In addition, all chemical shifts are given in parts per million relative to tetramethylsilane (TMS). Mass spectra were determined using an LTQ-Orbitrap XL (Thermo Fisher, USA).
7
NMR Spectroscopy of Nucleic Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All NMR experiments were recorded on Agilent Technologies DD2 600 MHz and VNMRS 800 MHz NMR spectrometers at 25°C unless stated otherwise. For suppression of the water signal, the double-pulsed field gradient spin echo (DPFGSE) pulse sequence was used. The translation diffusion coefficients were obtained with the use of pulse field gradient stimulated echo (PFG-STE) pulse sequence. Identification of guanine H1 protons in partially (8%) 13C and 15N site-specific labelled samples was acquired with 1D 15N-edited heteronuclear single quantum correlation (HSQC) experiment. Aromatic protons of adenines and guanines were identified with the use of 2D 13C-edited HSQC experiment. Non-exchangeable proton resonances were assigned using the 2D Nuclear Overhauser Effect SpectroscopY (NOESY) with mixing times (τm) of 80, 150 and 250 ms recorded on NMR samples in 100% D2O. 2D Total Correlation Spectroscopy (TOCSY) with τm of 80 ms, 2D Double Quantum Filtered Correlation SpectroscopY (DQF-COSY) and 2D 1H-31P COSY were used for cross-checking assignment of 2D NOESY spectra. Exchangeable proton resonances were assigned using 2D NOESY experiments with τm of 150 and 250 ms acquired on samples in 90% H2O, 10% D2O.
8
NMR Characterization of Yacon Syrup
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The samples were prepared by dissolving 10 mg of the yacon syrup into 550 μL of D 2 O (99.9%) with 1% of the trimethylsilylpropanoic acid, (TSP, v/m). The final solution was transferred to 5 mm NMR tubes. The NMR experiments were acquired in a spectrometer (Agilent, model DD2 600 MHz, Santa Clara, CA, USA) equipped with a 5 mm (H-F/ 15 N-31 P) inverse detection One Probe™, operating at 298 K. The spectra were recorded with 32 free induction decays (FID) into 21 K of data points for 13,157.9 Hz of spectral width, and relaxation delay of 2 s. For the molecular identification of the compounds, homo and heteronuclear 2D experiments were acquired. The spectra were referenced to the TMSP-d4 resonance at 0.0 ppm. The identification of the constituents within the yacon syrup was performed through 1 H-1 H COSY, 1 H-13 C HSQC, and 1 H-13 C HMBC experiments. The results were compared to the existing data in open access databases and literature reports.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!