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Buffered formaldehyde

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Buffered formaldehyde is a chemical solution used in various laboratory applications. It serves as a fixative, preserving the structure and composition of biological samples. The solution contains formaldehyde, a preservative, and a buffering agent to maintain a specific pH level. This product is commonly used in histology, cytology, and other areas of biological research and analysis.

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Lab products found in correlation

Standard microtome, by Leica (1 mentions) Dfc 3200 camera, by Leica (1 mentions) Ckx41 microscope, by Olympus (1 mentions) Prolong gold antifade with dapi, by Thermo Fisher Scientific (1 mentions) Fluoview fv1000 confocal microscope, by Olympus (1 mentions) Fv10 asw, by Olympus (1 mentions)

Spelling variants of this product

Formaldehyde (433 citations) Phosphate-buffered formaldehyde solution (5 citations)

4 protocols using buffered formaldehyde

1

Histological Analysis of Visceral Adipose and Liver

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Visceral adipose tissue and the liver from 9–12 month old Mito-Ob and their wild type littermates (as applicable) were fixed in buffered formaldehyde (Fisher Scientific, Ottawa, ON) and subsequently dehydrated, embedded in paraffin, and 5 μm sections were stained with hematoxylin-eosin or a protein specific antibody for immunohistochemistry35 (link)37 (link).
Ande S.R., Nguyen K.H., Grégoire Nyomba B.L, & Mishra S. (2016). Prohibitin-induced, obesity-associated insulin resistance and accompanying low-grade inflammation causes NASH and HCC. Scientific Reports, 6, 23608.
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2

Tissue Preparation and Histological Imaging

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Samples were fixed in 10% buffered formaldehyde (Fisher Chemicals, Fari Lawn, NJ), dehydrated in a series of ethanol baths (70, 95 and 100%) and finally in xylene, and embedded in paraffin. Tissue blocks were sectioned at 6 μm thickness using a standard microtome (Leica Biosystems, France), which were then subjected to standard H&E staining and imaged with a CKX41 microscope (Olympus, Japan) equipped with a Leica DFC 3200 camera.
Lin H., Tang Y., Lozito T.P., Oyster N., Kang R.B., Fritch M.R., Wang B, & Tuan R.S. (2017). Projection Stereolithographic Fabrication of BMP-2 Gene-activated Matrix for Bone Tissue Engineering. Scientific Reports, 7, 11327.
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3

Immunohistochemical Analysis of Bone Tissue

Cited 1 time
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Bone tissue samples were placed into cassettes, preserved in buffered formaldehyde (Fisher, Kalamazoo, MI), and embedded in paraffin wax. Five micron-thick sections were cut on a microtome after incubating embedded paraffin blocks in decalcification solution (Decal Stat) (Decal Chemical Corp, Tallman, NY) to dissolve mineralized bone. Tissue sections were mounted and baked onto slides. Target retrieval using citrate buffer was done as described previously (31 (link)). Immunohistochemistry (IHC) was carried out using rabbit anti-SDF1 antibody (clone RB32982) which reacted with both human and sheep tissue sections (Abgent, San Diego, CA), and/or mouse anti-human nuclei antibody (clone 235-1) (PhosphoSolutions, Aurora, CO) which only reacted with human cells. Secondary antibodies included donkey-anti-rabbit Alexa Fluor 647 (red) and donkey-anti-mouse Alexa Fluor 488 (green) (Jackson ImmunoResearch Laboratories West Grove, PA). Nuclei were stained using slide mounting media (Prolong Gold antifade with DAPI) (Invitrogen). Photo-micrographs were taken on an Olympus Fluoview FV1000 confocal microscope with UPlanFLN 40x1.30 numeric aperture oil objective lens, using FV10-ASW version 01.05.00.14 software (Olympus America Inc., Melville, NY, USA). Images were processed using Adobe Photoshop, version CS5.
Goodrich A.D., Varain N.M., Jeanblanc C.M., Colon D.M., Kim J., Zanjani E.D, & Hematti P. (2014). Influence of a dual-injection regimen, plerixafor, and CXCR4 on in-utero hematopoietic stem cell transplantation and engraftment using the sheep model. Cytotherapy, 16(9), 1280-1293.
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4

Tissue Histology after Oxybutynin Instillation

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Samples of tissue were taken from bladders after 60 min intravesical instillation of oxybutynin chloride (undiluted and urine -diluted groups). Samples were fixed in 4 % buffered formaldehyde (Fisher Scientific UK Ltd, Leicestershire, UK) for 48 h at room temperature. Tissues were embedded in paraffin, sectioned at 10 µm thickness and stained with Mason's trichrome prior to examination under light microscopy.
Williams N.A., Lee K.M., Allender C.J., Bowen J.L., Gumbleton M., Harrah T., Raja A, & Joshi H.B. (2015). Investigating detrusor muscle concentrations of oxybutynin after intravesical delivery in an ex vivo porcine model. Journal of pharmaceutical sciences, 104(7).
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