The Waters Acquity UPLC H-Class system (Waters, Milford, MA, USA) included a quaternary solvent manager, an autosampler set at 4 °C and a Waters Acquity UPLC Shield RP18 column (100 × 2.1 mm, 1.7 μm, Waters, Milford, MA, USA). The chromatographic separation was performed on isocratic elution with mobile phases of acetonitrile (A) and 0.1% phosphoric acid (B). Solvent A was 22% (v/v) at the beginning, linearly increased to 42% over 1 min, and then returned to 22% by 2 min at a flow rate of 0.3 ml/min. The detection was performed by a photodiode array detector at 327 nm.
Waters acquity uplc shield rp18 column
Manufactured by Waters Corporation
Sourced in United States
The Waters Acquity UPLC Shield RP18 column is a reversed-phase liquid chromatography column designed for high-performance separations. It features a bonded C18 stationary phase and is compatible with ultra-high-performance liquid chromatography (UPLC) systems.
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2 protocols using waters acquity uplc shield rp18 column
1
UPLC-PDA Analysis of Pharmaceuticals
2
UPLC Determination of QR in Plasma
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The Waters Acquity UPLC H-Class system (Waters, Milford, MA, USA) includes a four-stage solvent manager, an autosampler set at 4°C and a Waters Acquity UPLC Shield RP18 column (100 × 2.1 mm, 1.7 μm, Waters. Milford, MA, USA). The mobile phase was methanol-0.1% phosphate (72:28, v/v). Detection was performed by a photodiode array detector at 373 nm at a flow rate of 0.2 mL/min. This assay was developed for the determination of QR in blood plasma.
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