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Mm00725412 s1

Manufactured by Thermo Fisher Scientific
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Mm00725412_s1 is a laboratory assay product. It is designed to facilitate specific and quantitative detection of a target biological analyte. The product's core function is to enable accurate measurement and analysis of the target analyte in research or diagnostic applications.

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Lab products found in correlation

Abi viia 7, by Thermo Fisher Scientific (1 mentions) Mm00487032 m1, by Thermo Fisher Scientific (1 mentions) Gapdh mm99999915 g1, by Thermo Fisher Scientific (1 mentions) Taqman universal pcr master mix, by Thermo Fisher Scientific (1 mentions) Taqman gene expression assay, by Thermo Fisher Scientific (1 mentions) α actin, by Thermo Fisher Scientific (1 mentions) Mm99999915 g1, by Thermo Fisher Scientific (1 mentions) Taqman reverse transcription reagent, by Thermo Fisher Scientific (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) Mm00434228 m1, by Thermo Fisher Scientific (1 mentions) Mm00446190 m1, by Thermo Fisher Scientific (1 mentions) Taqman array 96 well plate, by Thermo Fisher Scientific (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Steponeplus, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions)

Close spelling variants of this product

TaqMan Gene Expression Assays Mm00725412_s1

3 protocols using mm00725412 s1

1

Gene Expression Profiling of Lipid Signaling

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The TaqMan gene expression assay and TaqMan Universal PCR Master Mix (4444556, Applied Biosystems, Foster City, CA, USA) RT-PCR reaction kit were used on an ABI Viia 7 real-time PCR system (Applied Biosystems, Foster City, CA, USA). The delta CT method normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used to analyze receptor expression. The change in expression was calculated using the 2-ΔΔCT method normalized to GAPDH as an endogenous control. The primers used were Mm99999064_m1 (IL6), Mm01200332_g1 (Enpp2), Mm01346925_m1 (LPAR1), Mm00469562_m1 (LPAR2), Mm00469694_m1 (LPAR3), Mm02620784_s1 (LPAR4), Mm02621109_s1 (LPAR5), Mm00725412_s1 (Acta2), Mm00487032_m1 (Cnn1), Mm00441661_g1 (Tagln), Mm00443013_m1 (Myh11), and Mm99999915_g1 (GAPDH), which were obtained from Applied Biosystems (Foster City, CA, USA).
Subedi U., Manikandan S., Bhattarai S., Sharma P., Sharma S., Sun H., Miriyala S, & Panchatcharam M. (2023). The Autotaxin-LPA Axis Emerges as a Novel Regulator of Smooth Muscle Cell Phenotypic Modulation during Intimal Hyperplasia. International Journal of Molecular Sciences, 24(3), 2913.
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2

Real-time RT-PCR for Gene Expression

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Real-time RT-PCR was performed as described19 (link). In brief, total RNA was extracted from cultured BMC. Total RNA (250 ng) was reverse transcribed in 25ul of reaction mixture using Taqman Reverse Transcription reagents (Applied Biosystems). Primers for PDGFR-β, α-actin and GAPDH were purchased from Applied Biosystems (Mm00435544_m1, Mm00725412_s1, and Mm99999915_g1, respectively). Real time PCR was performed using Mx3000™ (STRATAGENE). Every transcript was normalized with GAPDH transcripts.
Sakihama H., Lee G.R., Chin B.Y., Csizmadia E., Gallo D., Qi Y., Gagliani N., Wang H., Bach F.H, & Otterbein L.E. (2021). Carbon Monoxide Suppresses Neointima Formation in Transplant Arteriosclerosis by Inhibiting Vascular Progenitor Cell Differentiation. Arteriosclerosis, thrombosis, and vascular biology, 41(6), 1915-1927.
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3

Quantitative Analysis of Gene Expression in Colonic and Liver Tissues

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Pieces of the colonic wall and non-cancerous liver specimen were homogenized, and total RNA was isolated using an RNeasy Mini Kit (Qiagen, Hilden, Germany) in accordance with the manufacturer's statement. Then, the specimens were used for quantitative analysis of gene expression using reverse-transcriptase PCR (qRT-PCR). cDNA was prepared using a reverse transcription kit (High Capacity cDNA Reverse Transcription Kits ; Applied Biosystems, Foster City, CA, USA). GAPDH (Applied Biosystems) was used as an endogenous control. Inter-leukin-1β (IL1B), interleukin-6 (IL6), and alpha actin 2 (ACTA2) in the liver, and claudin 1 (CLDN1) in the colon were assessed using TaqMan gene expression assays (IL6, Mm00446190_ m1 ; IL1B, Mm00434228 _m1 ; ACTA2, Mm00725412 _ s1 ; CLDN1 ; Mm00516701_m1 : Applied Biosystems). TaqMan gene expression assays were conducted in duplicate in 20 mL reactions using TaqMan Array 96-well plates and a real-time PCR System (StepOnePlus ; Applied Biosystems) following the manufacturer's statement. Standard curves were made from three-fold serial dilutions of cDNA, and the copy numbers of target genes were calculated in accordance with the standard curves (21) .
Yamada S., Morine Y., Imura S., Ikemoto T., Saito Y., Shimizu M., Tsuneyama K., Nishiyama M., Ishizawa S, & Shimada M. (2023). Effect of daikenchuto (TU-100) on carcinogenesis in non-alcoholic steatohepatitis. The journal of medical investigation : JMI, 70(1.2).
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