Imagequant las 4010 digital imaging system

Manufactured by GE Healthcare

The ImageQuant LAS 4010 Digital Imaging System is a lab equipment designed for digital imaging analysis. It can be used to capture and analyze images of various samples, such as gels, blots, and microplates. The system features a high-resolution camera, adjustable lighting, and software for image processing and quantification.

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Lab products found in correlation

Bis tris sds page, by Thermo Fisher Scientific (2 mentions) Dc protein assay kit, by Bio-Rad (2 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Cell Signaling Technology (1 mentions) Supersignal west pico, by Thermo Fisher Scientific (1 mentions) Fast protease inhibitor, by Merck Group (1 mentions) Anti cxcr5, by Bioss Antibodies (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti gapdh, by Abcam (1 mentions) Non fat milk, by Bio-Rad (1 mentions) Ripa buffer, by Merck Group (1 mentions)

2 protocols using imagequant las 4010 digital imaging system

Western Blot Analysis of Retinal and RPE/Choroid Proteins

Cited 1 time

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WB was performed as previously described with some modifications [41 (link)]. Dissected mouse retina or RPE/choroid was sonicated in cold RIPA buffer containing FAST Protease Inhibitor (Sigma). Protein content from the retina or RPE/choroid was quantified using the Bio-Rad DC Protein Assay kit (Hercules, CA). 5–20 μg protein per lane was separated by 4–12% Bis-Tris SDS-PAGE (Life Technologies) and transferred to 0.2 μm pore size nitrocellulose membranes. Membranes were blocked with 5% non-fat milk at room temperature for 1 h and then incubated overnight at 4°C with the following primary antibodies: anti-Cxcr5 (1:500, Bioss), anti-ZO-1 (1:500, DSHB), anti-TNF-α (1:500, Janssen, PA), anti-GAPDH (1:2500, Abcam), and anti-β-actin (1:2500, Cell Signaling) followed by incubation with horseradish-peroxidase (HRP)-conjugated secondary antibody (1:4000; Cell Signaling) for 1 h at room temperature. Signal was detected by enhanced chemiluminescence (ECL) using SuperSignal West Pico or Femto kit (Thermo Scientific) and GE Healthcare's ImageQuant LAS 4010 Digital Imaging System (Pittsburgh, PA). Densitometry was performed using Image J (NIH, Bethesda, MD).

Huang H., Liu Y., Wang L, & Li W. (2017). Age-related macular degeneration phenotypes are associated with increased tumor necrosis-alpha and subretinal immune cells in aged Cxcr5 knockout mice. PLoS ONE, 12(3), e0173716.

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Immunoblotting Analysis of Cellular Proteins

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ARPE-19 cells were washed with cold PBS, scraped off wells into RIPA buffer containing Sigma FAST Protease Inhibitor, and lysed on ice. Dissected mouse tissues (retina or RPE/choroid) were sonicated in cold RIPA buffer (Sigma) containing Sigma FAST Protease Inhibitor. Protein content from ARPE-19 cells or mouse tissue (retina or RPE/choroid) was quantified using the BioRad DC Protein Assay kit (Hercules, CA). For immunoblotting, 5-20μg protein per lane was separated by 4-12% Bis-Tris SDS-PAGE (Life Technologies) and transferred to 0.2μm pore nitrocellulose membranes. Membranes were blocked with 5% non-fat milk (BioRad) at room temperature for 1hr and then incubated with primary antibodies at 4°C overnight. After washing with 1×TBS-T (20mM Tris, 136.8mM NaCl, pH7.6, with 0.1% v/v Tween 20) three times for 10min, the blots were incubated with secondary antibodies (1:5000) at room temperature for 45min, and blots were visualized with enhanced chemiluminescence (ECL; Thermo Fisher Scientific, Inc., Waltham, MA). Images were captured and analyzed using GE Healthcare’s ImageQuant LAS 4010 Digital Imaging System (Pittsburgh, PA).

Wang L., Cano M, & Handa J.T. (2014). p62 Provides Dual Cytoprotection Against Oxidative Stress in the Retinal Pigment Epithelium. Biochimica et biophysica acta, 1843(7), 1248-1258.

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