Kindlin 2 antibody

The protein extraction and Western blot analysis were performed as previous described.²² The antibody information was as following: kindlin‐2 antibody (13562s, Cell Signaling Technology), Bax antibody (14796, Cell Signaling Technology), Bcl‐2 antibody (ab194583, Abcam), GRP78 antibody (GRP78, 3183, Cell Signaling Technology), CHOP antibody (2895, Cell Signaling Technology), PDI antibody (3501, Cell Signaling Technology), Ero1‐Lα antibody (ab81959, Abcam), Drp‐1 antibody (ab184247, Abcam), Tfam antibody (ab131607, Abcam), ND3 (ab192306, Abcam), Mfn‐2 (9482, Cell Signaling Technology), Fis‐1 (ab71498, Abcam), ATPB (ab170947, Abcam), β actin Antibody (HRP‐60008, Proteintech), Goat anti‐rabbit IgG antibody (SA00001‐2, Proteintech) and Goat anti‐mouse IgG antibody (SA00001‐1, Proteintech). The Image J software was used for quantitative analysing, and relative protein levels were expressed as the intensity ratio of target protein and β actin.

The MIA PaCa-2 cells used in this study were obtained from ATCC. Cell culture media and supplements, Dulbecco’s modified Eagle’s medium (DMEM) (MT10090CV; Corning, New York, USA) fetal Bovine serum (FBS) (F0926; Millipore Sigma, St. Louis, MO, USA), Horse serum (30-2041; ATCC, Manassas, VA, USA) and antibiotic antimycotic solution (30-004-Cl; Corning, New York, USA) were used. The primary antibodies used were Integrin β1 (D2E5) Rabbit mAb (9699; Cell Signaling Danvers, MA, USA), Integrin α5 antibody (4705; cell Signaling), Paxillin antibody (2542; Cell Signaling, Danvers, MA, USA), Phospho-Paxillin (Tyr118) antibody (2541; Cell Signaling), Kindlin-2 antibody (13562; Cell Signaling), TGFβR2 (E-6) (sc-17792; Santa Cruz Biotechnology, Dallas, TX, USA), Smad2/3 (A-11) (sc-393312; Santa Cruz Biotechnology, Dallas, TX, USA), Phospho-Smad2/3 (D27F4) Rabbit mAb (8828; Cell Signaling, Danvers, MA, USA).