Kaarksapatgg

Manufactured by AnaSpec

KAARKSAPATGG is a custom peptide synthesized by AnaSpec. It is a 10-amino acid sequence that can be used in various biochemical and biomedical applications. The detailed specifications and intended use of this product are not available.

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Lab products found in correlation

2 protocols using kaarksapatgg

Histone Modifications and Chromatin Interactions

Cited 2 times

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Rabbit polyclonal to H1, HMGN1, HMGN2, and H3 were from our laboratory, anti H3K27ac (Abcam#ab4729), anti H3K27me3 (Abcam#ab6002), monoclonal anti H1(Milipore-Sigma #05-457), anti CEBPB (Abcam#ab32358), Anti-Brd3 (Active Motif #61489), Anti-Brd4 (Bethyl Laboratories #A301-985A100), Anti-CEBPB (Abcam #ab32358), Anti-CTCF (EMD Millipore #07-729), Anti-Ets1 (Active Motif #39580), Anti-Ikaros (Active Motif #39355), Anti-Irf8 (Bethyl Laboratories #A304-027A), Anti-Klf4 (Abcam #106629), Anti-Nanog (Active Motif #61419), Anti-Oct4 (Abcam #ab19857), Anti-p300 (Active Motif #61401), Anti-Pax5 (Abcam #183575), Anti-Sox2 (Abcam #97959).
The following recombinant mononucleosomes were purchased from Active Motif: unmodified (#81070); H3K27me3 modified (#81834), H3K27ac modified (#81077).
Wild type and HMGN DKO mouse embryonic fibroblasts, embryonic stem cell lines^{55 (link)} and resting B cells^{56 (link)} were as previously described. Peptides Histone H3 (23–34) peptide, KAARKSAPATGG and Histone H3K27ac (23–34) peptide, KAAR - K(Ac)—SAPATGG were from AnaSpec, Inc.

Zhang S., Postnikov Y., Lobanov A., Furusawa T., Deng T, & Bustin M. (2022). H3K27ac nucleosomes facilitate HMGN localization at regulatory sites to modulate chromatin binding of transcription factors. Communications Biology, 5, 159.

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Chromatin Immunoprecipitation with Recombinant Nucleosomes

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Antibodies, recombinant nucleosomes, peptides and cell lines.
Rabbit polyclonal to H1, HMGN1, HMGN2 and H3 were from our laboratory, anti H3K27ac (Abcam#ab4729), anti H3K27me3 (Abcam#ab6002), monoclonal anti H1(Milipore-Sigma #05-457), anti CEBPB (Abcam#ab32358), Anti-Brd3 (Active Motif #61489), Anti-Brd4 (Bethyl Laboratories #A301-985A100), Anti-CEBPB (Abcam #ab32358), Anti-CTCF (EMD Millipore #07-729), Anti-Ets1 (Active Motif #39580), The following recombinant mononucleosomes were purchased from Active Motif : unmodified (#81070); H3K27me3 modified (#81834), H3K27ac modified (#81077).
Wild type and HMGN DKO mouse embryonic fibroblasts, embryonic stem cell lines (Deng et al. 2013 ) and resting B cells (Zhang et al.) were as previously described.
Peptides Histone H3 (23 -34) peptide, KAARKSAPATGG and Histone H3K27ac (23 -34) peptide, KAAR -K(Ac) -SAPATGG were from AnaSpec, Inc.

Zhang S., Postnikov Y.V., Lobanov A., Furusawa T., Deng T., & Bustin M. (2021). H3K27 Acetylated Nucleosomes Facilitate HMGN Protein Localization at Regulatory Sites to Modulate The Interaction of Transcription Factors with Chromatin.

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