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Dcfh da reactive oxygen species assay kit

Manufactured by Meilun
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Sourced in United States, China

The DCFH-DA Reactive Oxygen Species Assay Kit is a fluorometric tool used to detect and quantify the presence of reactive oxygen species (ROS) in various cell types and samples. The kit contains 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA), which is a cell-permeable compound that can be oxidized by ROS to produce a fluorescent signal. The intensity of the fluorescent signal is proportional to the level of ROS in the sample.

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Lab products found in correlation

Facs flow cytometer, by BD (1 mentions) Annexin 5 fitc pi apoptosis assay kit, by BD (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Annexin 5 pe 7 aad apoptosis detection kit, by BD (1 mentions)

Close spelling variants of this product

DCFH-DA

2 protocols using dcfh da reactive oxygen species assay kit

1

Apoptosis and Necrosis Assay

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To examine the morphology of apoptotic and necrotic cells, the cells were seeded in 6-well plates at ~70% confluency and subjected to the indicated treatments. For flow cytometry analyses, cells were treated with various chemotherapy drugs as indicated. Cells were harvested, washed with PBS three times, and stained using Annexin V-FITC/PI Apoptosis Assay Kit (BD Pharmingen, San Jose, CA, USA) and DCFH-DA Reactive Oxygen Species Assay Kit (Meilunbio, China) according to the manufacturer’s instructions. Stained cells were analyzed with a BD FACS flow cytometer (BD Biosciences, Franklin Lakes, NJ), and the data were processed using FlowJo software.
Chen Y., Xie X., Wang C., Hu Y., Zhang H., Zhang L., Tu S., He Y, & Li Y. (2020). Dual targeting of NUAK1 and ULK1 using the multitargeted inhibitor MRT68921 exerts potent antitumor activities. Cell Death & Disease, 11(8), 712.
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2

Apoptosis and ROS Measurement Assay

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For apoptosis analysis, the indicated cells were treated as described in 6-well plates, and apoptosis of floating and attached cells was detected with an Annexin V-PE/7-AAD Apoptosis Detection Kit (BD, CA, USA). For measurement of total ROS, the collected attached cells were analyzed with a DCFH-DA Reactive Oxygen Species Assay Kit (Meilunbio, Dalian, China) according to the instructions of the manufacturer. These assays were conducted and analyzed with a BD LSRFortessaTM flow cytometer and the included software.
Xu S., Ma Y., Tong Q., Yang J., Liu J., Wang Y., Li G., Zeng J., Fang S., Li F., Xie X, & Zhang J. (2021). Cullin-5 neddylation-mediated NOXA degradation is enhanced by PRDX1 oligomers in colorectal cancer. Cell Death & Disease, 12(3), 265.
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