Methoxy poly(ethylene glycol) (mPEG) (MW 5000), poly(ethylene glycol) (PEG) (MW 400), dipalmitoylphosphatidylcholine (DPPC), cholesterol, 4,4′-azobis(4-cyanovaleric acid) (ABCPA), l -lysine (Lys), and phosphotungstic acid (PTA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methacrylic acid (MAAc) and ammonium sulfate ((NH4)2SO4) were obtained from Acros Organics (Geel, Belgium). N-hydroxysuccinimide (NHS ester) was purchased from Alfa Aesar (Heysham, Lancashire, UK). Doxorubicin (Dox) was purchased from LC Laboratories (Woburn, MA, USA). Methanol and dichloromethane (DCM) were purchased from ECHO (Miaoli, Taiwan). The fluorescent dye cyanine 5.5-NHS ester (Cy5.5-NHS ester) was obtained from Lumiprobe (Hunt Valley, MD, USA). Fetal bovine serum (FBS) and LysoTracker Red DND-99 fluorescent dye were received from Thermo Fisher Scientific (Waltham, MA, USA). The PD-10 desalting column and Sephadex G 50 column were purchased from GE Healthcare Life Sciences (Little Chalfont, Buckinghamshire, UK).
Dipalmitoylphosphatidylcholine dppc
Manufactured by Merck Group
Sourced in United States
Dipalmitoylphosphatidylcholine (DPPC) is a phospholipid that is a major component of lung surfactant. It is a widely used model system for the study of membrane properties and behavior.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Bronchial epithelial growth medium (begm), by Lonza (2 mentions)
Fetal bovine serum (fbs), by Gemini Bio (2 mentions)
Low endotoxin bovine serum albumin, by Gemini Bio (2 mentions)
Ammonium molybdate, by Merck Group (2 mentions)
Sodium sulfite, by Merck Group (2 mentions)
Sulfuric acid, by Thermo Fisher Scientific (2 mentions)
Sephadex g25 gel, by Merck Group (2 mentions)
Chloroform, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Hydrogen peroxide, by Merck Group (2 mentions)
Triton x 100, by Merck Group (2 mentions)
Trizma base, by Merck Group (2 mentions)
Sodium bisulfite, by Merck Group (2 mentions)
Pd 10 desalting column, by GE Healthcare (1 mentions)
Phosphotungstic acid pta, by Merck Group (1 mentions)
Methacrylic acid maac, by Thermo Fisher Scientific (1 mentions)
Sephadex g 50 column, by GE Healthcare (1 mentions)
Doxorubicin dox, by LC Laboratories (1 mentions)
Distearoylphosphatidylcholine dspc, by Merck Group (1 mentions)
8 protocols using dipalmitoylphosphatidylcholine dppc
1
Multifunctional Nanoparticle Synthesis and Drug Delivery
2
Bronchial Epithelial Cell Culture Protocol
3
Magnetoliposome Preparation Protocol
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Ultrapure water Milli-Q grade (MilliporeSigma, St. Louis, MO, USA) and spectroscopic grade solvents were used in all the synthesis procedures. pH buffer stock solutions in the pH range between 2 and 11 were prepared from a sodium phosphate 0.1 M solution, adjusting the pH by mixing properly a solution containing citric acid (0.05 M) and boric acid (0.2 M) in ultrapure water (all reagents from Sigma-Aldrich, St. Louis, MO, USA), following [30 (link)]. The pH values were measured with a mini-pH meter NiCd-1 (Mettler-Toledo, Greifensee, Switzerland) and the pH of each solution was properly adjusted using a solution of HCl and NaOH (1 M) (all reagents from Sigma-Aldrich, St. Louis, MO, USA). For magnetoliposomes preparation, the lipids dipalmitoylphosphatidylcholine (DPPC) (from Sigma-Aldrich, St. Louis, MO, USA), distearoylphosphatidylcholine (DSPC) (from Sigma-Aldrich, St. Louis, MO, USA), dioleoylphosphatidylethanolamine (DOPE) (from Sigma-Aldrich, St. Louis, MO, USA), cholesteryl hemisuccinate (CHEMS) (from Sigma-Aldrich, St. Louis, MO, USA) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000, from Avanti Polar Lipids, Birmingham, AL, USA) were used.
4
Preparation and Purification of Synthetic Surfactant Peptides
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The sequence of CPVHLKRLLLLLLLLLLLLLLLL was chosen to prepare the SP-C peptide analog.15 (link) The synthesized peptide was obtained from Anygen Co., Ltd. (Jangseong, Korea). As PS is composed mostly of phospholipids, we used dipalmitoyl phosphatidylcholine (DPPC, Sigma-Aldrich Corp., St. Louis, MO, USA), phosphatidylglycerol (PG, Sigma-Aldrich Corp., St. Louis, MO, USA), and palmitic acid (PA, Sigma-Aldrich Corp., St. Louis, MO, USA). We mixed DPPC, PG, PA, and SP-C 75:25:10:3 (w/w) and lyophilized the mixture to obtain sSP-C PS powder.18 (link) To obtain PNBS, intact lungs of healthy cows were extracted. Normal saline (37℃) was instilled via the airway and re-suctioned after several rounds of gentle massage. Twenty liters of normal saline was used for each pair of lungs (approximately 5 kg). The obtained fluid was centrifuged at 500 g for 10 min to remove tissue remnants and blood cells. Then, a 15000 g centrifuge was applied over 60 min at 4℃ to obtain a PS pellet. To purify this crude natural surfactant (CNS), 4-5 mL of CNS pellet was diluted in 0.9% saline to be 30 mL of emulsion, and 48 mL of 26% sodium bromide solution was added. This was centrifuged at 24000 rpm for 120 minutes. In the middle, a white thin layer was obtained and mixed with normal saline. The pellet after 10 minutes of 500 g centrifugation was obtained and used as PNBS.16 19 20 (link)
5
Low-Frequency Focused Ultrasound Instrument
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A DZC-type low-frequency focused ultrasound instrument was developed and provided by the Ultrasound Research Institute of Chongqing Medical University (Chongqing, China, Fig. 2 ). Diphenylphosphoryl azide (DPPA), distearoylphosphatidylethanolamine (DSPE) and dipalmitoylphosphatidylcholine (DPPC) were purchased from Sigma (USA); RPMI-1640 and fetal bovine serum were purchased from HyClone (Beijing, China). Mouse anti-human CD34 monoclonal antibodies were purchased from Zhongshan Biological Technology (Beijing, China), and a TUNEL staining kit was purchased from Roche (Basel, Switzerland).![]()
The DZC low-frequency focused ultrasound instrument
6
Bronchial Epithelial Cell Culture Protocol
7
Cyclodextrin-Mediated Drug Formulation
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α-CD, β-CD, γ-CD, and randomly methylated-β-CD (RAMEB, DS = 12.9), were provided by Wacker Chemie (Germany). Low methylated-β-CD (CRYSMEB, DS = 4.9) and hydroxypropyl-β-CD (HPBCD, DS = 5.6) were provided by Roquette Frères (Lestrem, France). Sulfobutyl ether-β-CD (SBE-β-CD, DS = 6.5) was provided by LIGAND Pharmaceuticals (San Diego, CA, USA). Dipalmitoylphosphatidylcholine (DPPC) and trizma base (buffer reagent) were purchased from Sigma-Aldrich, Switzerland. Triton X-100, sodium chloride (NaCl), and Sephadex G25 gel were purchased from Sigma-Aldrich, Belgium. Ammonium molybdate, hydrogen peroxide, potassium dihydrogen phosphate, sodium sulfite, sodium bisulfite, chloroform, and methanol were purchased from Sigma-Aldrich, Germany. Cholesterol and sulforhodamine B were purchased from Sigma-Aldrich, USA. 4-Amino-3-hydroxy-1-naphthalene sulfonic acid was purchased from Fluka, India. Sulfuric acid was purchased from ACROS Organics, Belgium and diethyl ether was purchased from VWR-Prolabo Chemicals, Belgium.
8
Liposome Preparation and Characterization
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Dipalmitoylphosphatidylcholine (DPPC) (≥ 99%) and trizma base (buffer reagent) were purchased from Sigma-Aldrich, Swiss. Triton X-100, sodium chloride (NaCl) and Sephadex G25 gel were purchased from Sigma-Aldrich, Belgium. Sodium azide, ammonium molybdate, hydrogen peroxide, potassium dihydrogenophosphate, sodium sulfite, sodium bisulfite, menthol, eugenol, chloroform and methanol were purchased from Sigma-Aldrich, Germany. Linalool was purchased from Sigma-Aldrich, Switzerland. Trans-Anethole was purchased from Sigma-Aldrich, Spain. Geraniol and nerolidol were purchased from Sigma-Aldrich, USA. Sulforhodamin B and camphor were purchased from Sigma-Aldrich, China. Borneol, bornyl acetate, p-cymene, ɑterpinene and ɣ-terpinene were purchased from Fluka Chemical, Switzerland. 4-amino-3hydroxyl-1-naphtalene sulfonic acid was purchased from Fluka, India. Anisole was purchased from Fluka, Germany. Guaiacol, thymol, estragole, isoeugenol were purchased from ACROS Organics, New Jersey, USA. The sulfuric acid was purchased from ACROS Organics, Belgium.
The diethylether was purchased from VWR-Prolabo Chemicals, Belgium. β-caryophyllene and eugenyl acetate were purchased from SAFC, USA. Linalyl acetate was purchased from SAFC, Spain. ɑ-Phellandrene was purchased from SAFC, Germany. Camphene was purchased from MERCK, Schurchardt.
The diethylether was purchased from VWR-Prolabo Chemicals, Belgium. β-caryophyllene and eugenyl acetate were purchased from SAFC, USA. Linalyl acetate was purchased from SAFC, Spain. ɑ-Phellandrene was purchased from SAFC, Germany. Camphene was purchased from MERCK, Schurchardt.
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