Human cells from liquid culture or cells from tissues of transplanted NSG mice were resuspended in FACS blocking buffer (D-PBS, 1%BSA, 5%FBS and Fc Block (1:100; BD Pharmingen, San Diego, CA)) at 4 °C for 15 minutes, and then incubated with following antibodies: APC anti-human CD45 (Invitrogen, Carlsbad, CA) or APC-Cy7 anti-human CD45 (BD Pharmingen); RPE anti-human CD34 (Dako, Carpinteria, CA) or Pe-Cy7 anti-human CD34 (BD Biosciences); APC anti-human CD38 (BD Pharmingen), Pacific blue anti-human CD19 (Invitrogen), PE anti-human CD13 (BD Pharmingen), Amcyan anti-human CD3 (BD Biosciences, San Jose, CA), RPE anti-human CD4 (Invitrogen), Pacific blue anti-human CD8 (Invitrogen); FITC anti-human CD15 (BD Biosciences) at 4 °C for 20 minutes. Cells were then washed and resuspended in fixing solution (D-PBS, 1% paraformaldehyde, and 2% FBS) and analyzed by FACS LSRII (BD Biosciences). 5,000–10,000 events were scored. Results were analyzed by FlowJo 8.5.3 software (Tristar, Ashland, OR).
Pe anti human cd13
Manufactured by BD
Sourced in United States
The PE anti-human CD13 is a laboratory reagent used for the identification and enumeration of CD13-positive cells in a sample. CD13, also known as aminopeptidase N, is a surface antigen expressed on various cell types, including myeloid cells, some lymphoid cells, and certain epithelial cells. This reagent can be utilized in flow cytometry applications to detect and quantify CD13-positive cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Apc cy7 anti human cd45, by BD (1 mentions)
Anti human cd38 apc, by BD (1 mentions)
Facs lsr 2, by BD (1 mentions)
Fc block, by BD (1 mentions)
Annexin 5 fitc and pi apoptosis detection kit, by Beyotime (1 mentions)
Hoechst 33342, by Beyotime (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Solarbio (1 mentions)
2 protocols using pe anti human cd13
1
Multiparameter Flow Cytometry Analysis
2
5-FU and Bestatin Combination Therapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
5-FU was purchased from Hisun Pharmaceutical (Zhejiang, China). Bestatin and 5-FU were dissolved in DMSO and diluted with saline solution or cell culture medium before use.
MTT [(3-[4, 5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide)] and L-leucine-p-nitro-anilide were from Solarbio (Beijing, China). PE anti-human CD13 was from BD (New York, the USA). Hoechst 33342 and an Annexin-V/FITC and PI apoptosis detection kit were purchased from Beyotime (Jiangsu, China). Microsomal aminopeptidase from porcine kidney microsomes was from Sigma-Aldrich (Shanghai, China). Male Kunming mice were supplied by the Experimental Animal Center of Shandong University. The ratio of combination of the two drugs means the molar concentration ratio.
MTT [(3-[4, 5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide)] and L-leucine-p-nitro-anilide were from Solarbio (Beijing, China). PE anti-human CD13 was from BD (New York, the USA). Hoechst 33342 and an Annexin-V/FITC and PI apoptosis detection kit were purchased from Beyotime (Jiangsu, China). Microsomal aminopeptidase from porcine kidney microsomes was from Sigma-Aldrich (Shanghai, China). Male Kunming mice were supplied by the Experimental Animal Center of Shandong University. The ratio of combination of the two drugs means the molar concentration ratio.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!